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The Journal of nutrition1992; 122(8); 1706-1716; doi: 10.1093/jn/122.8.1706

Increases in serum sphingosine and sphinganine and decreases in complex sphingolipids in ponies given feed containing fumonisins, mycotoxins produced by Fusarium moniliforme.

Abstract: Consumption of food contaminated with Fusarium moniliforme causes leucoencephalomalacia and hepatotoxicity in horses, pulmonary edema in pigs and liver cancer in rats, and has been correlated with esophageal cancer in humans. The causative agents are thought to be a family of compounds called fumonisins, which have recently been shown to be potent inhibitors of sphingosine (sphinganine) N-acyltransferase. Because inhibition at this step blocks the formation of complex sphingolipids while leading to accumulation of sphinganine, we hypothesized that exposure of animals to fumonisin-contaminated feed might be detected by analyses of serum sphingolipids. Within days of giving ponies feed contaminated with 15 to 44 micrograms/g fumonisin B1, there was an increase in the amount of free sphinganine (and sometimes sphingosine) and a reduction in complex sphingolipids. Free sphinganine and sphingosine decreased when ponies consumed less of the contaminated feed, and increased again when they consumed more fumonisin. When toxicosis was evident as indicated by other serum markers, complex sphingolipids as well as free sphingosine and sphinganine were elevated, probably due to loss of sphingolipids from dying cells. These findings establish that consumption of fumonisin-contaminated feed disrupts sphingolipid metabolism. Because the changes in sphinganine and sphingosine were seen before liver enzymes were noticeably elevated, they may be an early marker of exposure to fumonisins.
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Publication Date: 1992-08-11 PubMed ID: 1640265DOI: 10.1093/jn/122.8.1706Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates how consuming food contaminated with fumonisins, which are toxins produced by Fusarium moniliforme, alters the sphingolipid metabolism in ponies. The study found that exposure to fumonisins leads to an increase in free sphinganine and sphingosine and a decrease in complex sphingolipids within a few days, which could serve as an early indicator of fumonisin exposure.

Objective of the Research

  • The aim of the research was to determine if exposure to fumonisins, a type of mycotoxin produced by Fusarium moniliforme, could be identified through changes in serum sphingolipids in ponies.

Background Information

  • Fusarium moniliforme is a fungus that produces a family of toxins named fumonisins that cause leucoencephalomalacia and hepatotoxicity in horses, liver cancer in rats, and have been correlated with esophageal cancer in humans.
  • Fumonisins are robust inhibitors of sphingosine (sphinganine) N-acyltransferase. The interference of these inhibitors with this metabolic process hinders the production of complex sphingolipids and leads to the accumulation of sphinganine.

Research Method

  • The researchers administered feed, contaminated with varying concentrations of fumonisin B1, to ponies and monitored changes in their serum sphingolipids.

Results

  • Within a few days of consuming the contaminated feed, there was an increase in the amount of free sphinganine and sometimes sphingosine, and a decrease in complex sphingolipids.
  • When the consumption of contaminated feed was reduced, the levels of free sphinganine and sphingosine decreased, but increased again when more fumonisin was consumed.
  • When toxicosis became evident in ponies through other serum markers, there were elevations in complex sphingolipids and free sphingosine and sphinganine, probably as a result of dying cells releasing sphingolipids.

Conclusion

  • The findings indicate that the intake of fumonisin-contaminated feed disrupts sphingolipid metabolism in ponies.
  • Changes in sphinganine and sphingosine were observed before any significant increase in liver enzymes, suggesting that they could serve as early markers of exposure to fumonisins.

Cite This Article

APA
Wang E, Ross PF, Wilson TM, Riley RT, Merrill AH. (1992). Increases in serum sphingosine and sphinganine and decreases in complex sphingolipids in ponies given feed containing fumonisins, mycotoxins produced by Fusarium moniliforme. J Nutr, 122(8), 1706-1716. https://doi.org/10.1093/jn/122.8.1706

Publication

The Journal of nutrition
ISSN: 0022-3166
NlmUniqueID: 0404243
Country: United States
Language: English
Volume: 122
Issue: 8
Pages: 1706-1716

Researcher Affiliations

Wang, E
  • Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322.
Ross, P F
    Wilson, T M
      Riley, R T
        Merrill, A H

          MeSH Terms

          • Animal Feed
          • Animals
          • Chromatography, High Pressure Liquid
          • Food Contamination
          • Fumonisins
          • Fusarium / metabolism
          • Horses / blood
          • Kinetics
          • Liver / enzymology
          • Mycotoxins / pharmacology
          • Sphingolipids / blood
          • Sphingosine / analogs & derivatives
          • Sphingosine / blood

          Grant Funding

          • GM33369 / NIGMS NIH HHS

          Citations

          This article has been cited 22 times.
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