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Veterinary research communications2001; 25(7); 591-599; doi: 10.1023/a:1017981301328

Influence of plasma proteins on erythrocyte aggregation in three mammalian species.

Abstract: The aggregation capacity of human erythrocytes lies between that of the non-aggregating bovine erythrocytes and the remarkably aggregating equine ones. As the ability to aggregate is attributed to cell factors and the composition of the plasma proteins, the role that plasma proteins play in the aggregation process in these three species was studied. Washed erythrocytes were suspended in phosphate-buffered saline (PBS; pH 7.4, 300 mOsm/L) plus polyvinylpyrrolidone (PVP) in a suitable concentration to obtain an average intensity of aggregation (control media). The superimposed effect of replacing 80% of the medium by either autologous plasma, serum or albumin solution was studied. The plasma proteins appeared to enhance aggregation by human and equine erythrocytes, but impaired this process in bovine erythrocytes. Some evidence was obtained supporting the existence of serum factors capable of reducing aggregation of erythrocytes in cattle and it was concluded that the non-aggregating behaviour of bovine erythrocytes may be due to the cells interacting particularly with the macromolecules in the serum.
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Publication Date: 2001-10-05 PubMed ID: 11583382DOI: 10.1023/a:1017981301328Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates how plasma proteins affect the aggregation (clumping together) of red blood cells (erythrocytes) in humans, cows, and horses. The researchers found that while these proteins enhanced aggregation in human and horse cells, they disrupted this process in cow cells.

Objective of Research

This research was carried out to better understand the role of plasma proteins in the process of erythrocyte aggregation in three mammalian species: humans, cattle, and horses.

  • The researchers specifically wanted to investigate why human erythrocyte aggregation sits between that of non-aggregating bovine (cow) erythrocytes and highly aggregating equine (horse) erythrocytes.
  • The study aimed to lay bare the influence of plasma proteins on this aggregation process, given that the capacity to aggregate is attributed to cell factors and the composition of these proteins.

Research Method

  • The red blood cells of the three species were washed and suspended in a solution of phosphate-buffered saline (a type of salt solution); pH 7.4, 300 mOsm/L plus polyvinylpyrrolidone (PVP) at a specific concentration to achieve an average aggregation intensity.
  • The researchers then replaced 80% of this solution with either autologous plasma (the animal’s own plasma), serum, or albumin solution to explore the varying effects on erythrocyte aggregation.

Research Findings

  • The study found that plasma proteins actually advance the aggregation process in human and equine erythrocytes but hinder this process in bovine erythrocytes.
  • The researchers also found evidence of serum factors in cattle that are capable of reducing the aggregation of erythrocytes.
  • Therefore, the researchers concluded that the non-aggregating behaviour of bovine erythrocytes may be due to these cells interacting differently with macromolecules found in the serum.

Research Implications

  • This study contributes to the deeper understanding of how red blood cells behave in different species, especially concerning their ability to aggregate.
  • The findings concerning the role of plasma proteins and other serum factors can direct further studies into the biological and physiological differences between species, which may be useful in the field of veterinary or comparative medicine.

Cite This Article

APA
Spengler MI, Rasia M. (2001). Influence of plasma proteins on erythrocyte aggregation in three mammalian species. Vet Res Commun, 25(7), 591-599. https://doi.org/10.1023/a:1017981301328

Publication

Veterinary research communications
ISSN: 0165-7380
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 25
Issue: 7
Pages: 591-599

Researcher Affiliations

Spengler, M I
  • Catedra de Biofisica, Fac de Ciencias Médicas, Universidad Nacional de Rosario, Santa Fe, Rep Argentina.
Rasia, M

    MeSH Terms

    • Animals
    • Blood Proteins / pharmacology
    • Blood Proteins / physiology
    • Blood Viscosity
    • Cattle / blood
    • Culture Media
    • Erythrocyte Aggregation / drug effects
    • Erythrocyte Aggregation / physiology
    • Erythrocytes / drug effects
    • Erythrocytes / physiology
    • Horses / blood
    • Humans
    • Plasma Substitutes / pharmacology
    • Povidone / pharmacology
    • Species Specificity

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