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Home / Equine Research Bank / J Vet Sci / Integrated analysis of microRNA and mRNA expressions in peri...
Journal of veterinary science2017; 19(1); 99-106; doi: 10.4142/jvs.2018.19.1.99

Integrated analysis of microRNA and mRNA expressions in peripheral blood leukocytes of Warmblood horses before and after exercise.

Abstract: Exercise capacity is a valuable trait in horses, and it has been used as a horse selection criterion. Although exercise affects molecular homeostasis and adaptation in horses, the mechanisms underlying these effects are not fully described. This study was carried out to identify changes in the blood profiles of microRNAs (miRNAs) and mRNAs induced by exercise in horse leukocytes. Total RNAs isolated from the peripheral blood leukocytes of four Warmblood horses before and after exercise were subjected to next-generation sequencing (NGS) and microarray analyses to determine the miRNA and mRNA expression profiles, respectively. The expressions of 6 miRNAs, including 4 known and 2 novel miRNAs, were altered by exercise. The predicted target genes of the differentially expressed miRNAs identified by NGS were matched to the exercise-induced mRNAs determined by microarray analysis. Five genes (, , , , and ) from the microarray analysis were matched to the predicted target genes of the 6 miRNAs. The subset of mRNAs and miRNAs affected by exercise in peripheral blood leukocytes may be useful in elucidating the molecular mechanisms of exercise-associated physiology in horses.
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Publication Date: 2017-09-21 PubMed ID: 28927254PubMed Central: PMC5799405DOI: 10.4142/jvs.2018.19.1.99Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study to identify the changes in blood cells related to microRNA (miRNA) and mRNA induced by exercise in Warmblood horses, which can potentially reveal the molecular mechanisms of exercise-associated physiology in horses.

Objective of the Study

  • The primary aim of the study was to gain a better understanding of how exercise affects molecular homeostasis and adaptation in horses. It sought to identify changes in the blood profiles of miRNAs and mRNAs induced by exercise in horse leukocytes.

Methodology of the Study

  • The researchers conducted their study on Warmblood horses, renowned for their capabilities in equestrian sports. They isolated total RNAs from the peripheral blood leukocytes of four Warmblood horses both before and after exercise.
  • They used next-generation sequencing (NGS) and microarray analyses to discern both the miRNA and mRNA expression profiles, respectively, in these cells.

Findings of the Study

  • The study revealed that exercise altered the expression of 6 miRNAs. Of these, four were known miRNAs, and two were newly discovered in the course of this study.
  • The researchers identified the potential target genes of these differentially expressed miRNAs using NGS. To determine the mRNA patterns induced by exercise, the researchers used microarray analysis.
  • Five genes from this microarray analysis matched the predicted target genes of the six miRNAs identified in the study, thus linking the altered expression of these miRNAs to exercise.
  • The results of this study could help in understanding and possibly modifying the molecular adjustments in a horse’s body due to exercise.

Significance of the Study

  • The findings of this study increase our understanding of the physiological changes induced by exercise at the molecular level in horses. This could prove useful in developing strategies to maximize the performance and improve the health of horses through targeted exercise regimes.

Limitations of the Study

  • With the research conducted on a small sample of only four Warmblood horses, the findings need to be replicated on a larger scale before generalized conclusions can be drawn.

Cite This Article

APA
Kim HA, Kim MC, Kim NY, Ryu DY, Lee HS, Kim Y. (2017). Integrated analysis of microRNA and mRNA expressions in peripheral blood leukocytes of Warmblood horses before and after exercise. J Vet Sci, 19(1), 99-106. https://doi.org/10.4142/jvs.2018.19.1.99

Publication

Journal of veterinary science
ISSN: 1976-555X
NlmUniqueID: 100964185
Country: Korea (South)
Language: English
Volume: 19
Issue: 1
Pages: 99-106

Researcher Affiliations

Kim, Hang-Ah
  • Laboratory of Veterinary Clinical Pathology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
  • BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
Kim, Myung-Chul
  • Laboratory of Veterinary Clinical Pathology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
  • BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
Kim, Na-Yon
  • Laboratory of Veterinary Clinical Pathology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
  • BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
Ryu, Doug-Young
  • Laboratory of Environmental Health and Biomarkers, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
  • Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
Lee, Hong-Seok
  • Laboratory of Veterinary Clinical Pathology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
  • BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
Kim, Yongbaek
  • Laboratory of Veterinary Clinical Pathology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
  • Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.

MeSH Terms

  • Animals
  • Horses / genetics
  • Horses / metabolism
  • Leukocytes / metabolism
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Transcriptome

Conflict of Interest Statement

The authors declare no conflicts of interest.

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Citations

This article has been cited 6 times.
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