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Home / Equine Research Bank / J Bioenerg Biomembr / Interaction of horse heart cytochrome c with lipid bilayer m...
Journal of bioenergetics and biomembranes1997; 29(3); 211-221; doi: 10.1023/a:1022401825287

Interaction of horse heart cytochrome c with lipid bilayer membranes: effects on redox potentials.

Abstract: Cyclic voltammetry has been used to study the effects of interactions between horse cytochrome c and solid-supported planar lipid membranes, comprised of either egg phosphatidylcholine (PC) or PC plus 20 mol.% cardiolipin (CL), on the redox potential and the electrochemical electron transfer rate between the protein and a semiconductor electrode. Experiments were performed over a wide range of cytochrome c concentrations (0-440 microM) at low (20 mM) and medium (160 mM) ionic strengths. Three types of electrochemical behavior were observed, which varied as a function of the experimental conditions. At very low cytochrome c concentration (approximately 0.1 microM), and under conditions where electrostatic forces dominated the protein-lipid membrane interaction (i.e., low ionic strength with membranes containing CL), a redox potential (approximately 265 mV) and an electrochemical electron transfer rate constant (0.09 s[-1])were obtained which compare well with those measured in other laboratories using a variety of different chemical modifications of the working electrode. Two other electrochemical signals (not reported with chemically modified electrodes) were also observed to occur at higher cytochrome c concentrations with this membrane system, as well as with two other systems (membranes containing CL under medium ionic strength conditions, and PC only at low ionic strength). These involved positive shifts of the cytochrome c redox potential (by 40 and 60 mV) and large decreases in the electron transfer rate (to 0.03 and 0.003 s[-1]). The observations can be rationalized in terms of a structural model of the cytochrome c-membrane interaction, in which association involves both electrostatic and hydrophobic forces and results in varying degrees of insertion of the protein into the hydrophobic interior of the membrane.
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Publication Date: 1997-06-01 PubMed ID: 9298706DOI: 10.1023/a:1022401825287Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates how the interaction of horse heart cytochrome c with lipid membranes affects its redox potential using a method called cyclic voltammetry, demonstrating that variables like membrane composition, ionic strength, and protein concentration can influence the redox potential and electron transfer rate.

Research Methodology and Observations

  • The researchers employed cyclic voltammetry, a type of electrochemical measurement, to examine the impacts of interactions between horse cytochrome c and specific lipid membranes on the redox potential and electrochemical electron transfer rate.
  • The membranes were made of either egg phosphatidylcholine (PC) or a mixture of PC and cardiolipin (CL). The experiments were conducted at low to medium ionic strengths and varying levels of cytochrome c concentrations.
  • Three distinctive kinds of electrochemical behavior were observed, their occurrence was influenced by the specific experimental conditions.

Key Findings

  • In scenarios where electrostatic forces primarily dictated the interaction between the protein and lipid membrane (low ionic strength with membranes that contain cardiolipin), certain redox potential and electron transfer rate constants were noted. These values align with results obtained from other laboratories using different chemical modifications of the working electrode.
  • Two additional electrochemical signals were recorded at higher cytochrome c concentrations with specific membrane systems. These included positive shifts in cytochrome c redox potential and significant decreases in the electron transfer rate.

Interpretation and Model Proposal

  • The researchers offered an interpretation of their observations, suggesting that a combination of electrostatic and hydrophobic forces govern the association between the cytochrome c and the membrane. The degree of the protein’s insertion into the hydrophobic membrane interior varies, hence influencing the electrochemical behavior.
  • Consequently, the study proposed a structural model for the interaction between cytochrome c and the membrane, which can explain the observed changes in redox potential and electron transfer rate constants under different conditions.

Cite This Article

APA
Salamon Z, Tollin G. (1997). Interaction of horse heart cytochrome c with lipid bilayer membranes: effects on redox potentials. J Bioenerg Biomembr, 29(3), 211-221. https://doi.org/10.1023/a:1022401825287

Publication

Journal of bioenergetics and biomembranes
ISSN: 0145-479X
NlmUniqueID: 7701859
Country: United States
Language: English
Volume: 29
Issue: 3
Pages: 211-221

Researcher Affiliations

Salamon, Z
  • Department of Biochemistry, University of Arizona, Tucson 85721, USA.
Tollin, G

    MeSH Terms

    • Animals
    • Cytochrome c Group / metabolism
    • Electrochemistry
    • Electron Transport
    • Horses
    • Lipid Bilayers / metabolism
    • Models, Molecular
    • Myocardium / enzymology
    • Oxidation-Reduction
    • Stereoisomerism

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