English
French
Interleukin-1beta-induced extracellular matrix degradation and glycosaminoglycan release is inhibited by curcumin in an explant model of cartilage inflammation.
Abstract: Osteoarthritis (OA) is a degenerative and inflammatory disease of synovial joints that is characterized by the loss of articular cartilage, for which there is increasing interest in natural remedies. Curcumin (diferuloylmethane) is the main polyphenol in the spice turmeric, derived from rhizomes of the plant Curcuma longa. Curcumin has potent chemopreventive properties and has been shown to inhibit nuclear factor kappaB-mediated inflammatory signaling in many cell types, including chondrocytes. In this study, normal articular cartilage was harvested from metacarpophalangeal and metatarsophalangeal joints of eight horses, euthanized for reasons other than research purposes, to establish an explant model mimicking the inflammatory events that occur in OA. Initially, cartilage explants (N= 8) were stimulated with increasing concentrations of the proinflammatory cytokine IL-1beta to select effective doses for inducing cartilage degeneration in the explant model. Separate cartilage explants were then cotreated with IL-1beta at either 10 ng/mL (n= 3) or 25 ng/mL (n= 3) and curcumin (0.1 micromol/L, 0.5 micromol/L, 1 micromol/L, 10 micromol/L, and 100 micromol/L). After 5 days, the percentage of glycosaminoglycan (GAG) release from the explants was assessed using a dimethylmethylene blue colorimetric assay. Curcumin (100 micromol/L) significantly reduced IL-1beta-stimulated GAG release in the explants by an average of 20% at 10 ng/mL and 27% at 25 ng/mL back to unstimulated control levels (P < 0.001). Our results suggest that this explant model effectively simulates the proinflammatory cytokine-mediated release of articular cartilage components seen in OA. Furthermore, the evidence suggests that the inflammatory cartilage explant model is useful for studying the effects of curcumin on inflammatory pathways and gene expression in IL-1beta-stimulated chondrocytes.
Publication Date: 2009-09-03 PubMed ID: 19723086DOI: 10.1111/j.1749-6632.2009.04687.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article examines the beneficial effect of curcumin (main polyphenol in turmeric) on osteoarthritis (OA) by inhibiting inflammation and degradation in the cartilage. The study uses an explant model to emulate the inflammation occurring in OA.
Experiment Design
- The study employs normal articular cartilage harvested from the joints of eight horses that were euthanized for non-research purposes. The harvested cartilages were used to create an explant model simulating the inflammatory events in OA.
- Initially, the cartilage explants were exposed to escalating concentrations of the proinflammatory cytokine, IL-1beta, to identify effective doses that would induce cartilage degeneration in the model.
- Upon establishing the effective doses, other cartilages were treated with IL-1beta at different concentrations (10 ng/mL or 25 ng/mL) and combined with various curcumin concentrations (0.1 micromol/L to 100 micromol/L).
Procedure and Results
- The cartilages were observed for 5 days, after which the amount of glycosaminoglycan (GAG) release from the explants was estimated using a colorimetric assay, dimethylmethylene blue.
- It was found that curcumin at a concentration of 100 micromol/L notably reduced the release of GAG stimulated by IL-1beta by an average of 20% at 10 ng/mL and 27% at 25 ng/mL, bringing it back to unstimulated control levels.
Conclusion and Implications
- The results highlight that the in-vitro explant model effectively simulates the inflammatory cytokine-mediated processes of cartilage destruction seen in OA.
- Clinically, it showcases the potential of curcumin in mitigating OA inflammation by inhibiting the inflammatory pathways and gene expression in cartilage cells (chondrocytes) stimulated with IL-1beta.
- This study suggests that exploiting natural remedies like curcumin can pave the way for effective countermeasures against OA.
Cite This Article
APA
Clutterbuck AL, Mobasheri A, Shakibaei M, Allaway D, Harris P.
(2009).
Interleukin-1beta-induced extracellular matrix degradation and glycosaminoglycan release is inhibited by curcumin in an explant model of cartilage inflammation.
Ann N Y Acad Sci, 1171, 428-435.
https://doi.org/10.1111/j.1749-6632.2009.04687.x Publication
Researcher Affiliations
- Division of Veterinary Medicine, School of Veterinary Medicine and Science, University of Nottingham, Leicestershire, United Kingdom. Abigail.Clutterbuck@nottingham.ac.uk
MeSH Terms
- Animals
- Anti-Inflammatory Agents, Non-Steroidal / pharmacology
- Cartilage / drug effects
- Cartilage / metabolism
- Cartilage / pathology
- Curcumin / pharmacology
- Dose-Response Relationship, Drug
- Extracellular Matrix / metabolism
- Glycosaminoglycans / metabolism
- Horses
- Insulin-Like Growth Factor I / pharmacology
- Interleukin-1beta / toxicity
- Metacarpophalangeal Joint / drug effects
- Metacarpophalangeal Joint / metabolism
- Metacarpophalangeal Joint / pathology
- Osteoarthritis / chemically induced
- Osteoarthritis / metabolism
- Osteoarthritis / prevention & control
- Tissue Culture Techniques
Grant Funding
- BBSRC/S/M/2006/13141 / Biotechnology and Biological Sciences Research Council
Citations
This article has been cited 32 times.- Patnaik R, Jannati S, Sivani BM, Rizzo M, Naidoo N, Banerjee Y. Efficient Generation of Chondrocytes From Bone Marrow-Derived Mesenchymal Stem Cells in a 3D Culture System: Protocol for a Practical Model for Assessing Anti-Inflammatory Therapies.. JMIR Res Protoc 2023 Jul 28;12:e42964.
- Ashruf OS, Ansari MY. Natural Compounds: Potential Therapeutics for the Inhibition of Cartilage Matrix Degradation in Osteoarthritis.. Life (Basel) 2022 Dec 30;13(1).
- Keller LE, Tait Wojno ED, Begum L, Fortier LA. Regulatory T cells provide chondroprotection through increased TIMP1, IL-10 and IL-4, but cannot mitigate the catabolic effects of IL-1u03b2 and IL-6 in a tri-culture model of osteoarthritis.. Osteoarthr Cartil Open 2021 Sep;3(3):100193.
- Han G, Zhang Y, Li H. The Combination Treatment of Curcumin and Probucol Protects Chondrocytes from TNF-u03b1 Induced Inflammation by Enhancing Autophagy and Reducing Apoptosis via the PI3K-Akt-mTOR Pathway.. Oxid Med Cell Longev 2021;2021:5558066.
- Spinnen J, Shopperly LK, Rendenbach C, Ku00fchl AA, Sentu00fcrk U, Kendoff D, Hemmati-Sadeghi S, Sittinger M, Dehne T. A Novel Method Facilitating the Simple and Low-Cost Preparation of Human Osteochondral Slice Explants for Large-Scale Native Tissue Analysis.. Int J Mol Sci 2021 Jun 15;22(12).
- Sukhikh S, Noskova S, Ivanova S, Ulrikh E, Izgaryshev A, Babich O. Chondroprotection and Molecular Mechanism of Action of Phytonutraceuticals on Osteoarthritis.. Molecules 2021 Apr 20;26(8).
- Yabas M, Orhan C, Er B, Tuzcu M, Durmus AS, Ozercan IH, Sahin N, Bhanuse P, Morde AA, Padigaru M, Sahin K. A Next Generation Formulation of Curcumin Ameliorates Experimentally Induced Osteoarthritis in Rats via Regulation of Inflammatory Mediators.. Front Immunol 2021;12:609629.
- Houtman E, van Hoolwerff M, Lakenberg N, Suchiman EHD, van der Linden-van der Zwaag E, Nelissen RGHH, Ramos YFM, Meulenbelt I. Human Osteochondral Explants: Reliable Biomimetic Models to Investigate Disease Mechanisms and Develop Personalized Treatments for Osteoarthritis.. Rheumatol Ther 2021 Mar;8(1):499-515.
- Mehta S, Young CC, Warren MR, Akhtar S, Shefelbine SJ, Crane JD, Bajpayee AG. Resveratrol and Curcumin Attenuate Ex Vivo Sugar-Induced Cartilage Glycation, Stiffening, Senescence, and Degeneration.. Cartilage 2021 Dec;13(2_suppl):1214S-1228S.
- Buhrmann C, Honarvar A, Setayeshmehr M, Karbasi S, Shakibaei M, Valiani A. Herbal Remedies as Potential in Cartilage Tissue Engineering: An Overview of New Therapeutic Approaches and Strategies.. Molecules 2020 Jul 6;25(13).
- Ma W, Wang S, Xu H, Xie W, Bi R. The effect of curcuminoids for treating knee osteoarthritis: A protocol for systematic review and meta analysis.. Medicine (Baltimore) 2020 Jun 19;99(25):e20556.
- Jiang C, Luo P, Li X, Liu P, Li Y, Xu J. Nrf2/ARE is a key pathway for curcumin-mediated protection of TMJ chondrocytes from oxidative stress and inflammation.. Cell Stress Chaperones 2020 May;25(3):395-406.
- Guo Y, Tian L, Du X, Deng Z. MiR-203 regulates estrogen receptor u03b1 and cartilage degradation in IL-1u03b2-stimulated chondrocytes.. J Bone Miner Metab 2020 May;38(3):346-356.
- Pearson W, Kott LS. A biological extract of turmeric (Curcuma longa) modulates response of cartilage explants to lipopolysaccharide.. BMC Complement Altern Med 2019 Sep 11;19(1):252.
- Kim L, Kim JY. Chondroprotective effect of curcumin and lecithin complex in human chondrocytes stimulated by IL-1u03b2 via an anti-inflammatory mechanism.. Food Sci Biotechnol 2019 Apr;28(2):547-553.
- Mata M, Milian L, Oliver M, Zurriaga J, Sancho-Tello M, de Llano JJM, Carda C. In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study.. Stem Cells Int 2017;2017:8309256.
- Niazvand F, Khorsandi L, Abbaspour M, Orazizadeh M, Varaa N, Maghzi M, Ahmadi K. Curcumin-loaded poly lactic-co-glycolic acid nanoparticles effects on mono-iodoacetate -induced osteoarthritis in rats.. Vet Res Forum 2017 Spring;8(2):155-161.
- Wang J, Ma J, Gu JH, Wang FY, Shang XS, Tao HR, Wang X. Regulation of type II collagen, matrix metalloproteinase-13 and cell proliferation by interleukin-1u03b2 is mediated by curcumin via inhibition of NF-u03baB signaling in rat chondrocytes.. Mol Med Rep 2017 Aug;16(2):1837-1845.
- Zhang Z, Leong DJ, Xu L, He Z, Wang A, Navati M, Kim SJ, Hirsh DM, Hardin JA, Cobelli NJ, Friedman JM, Sun HB. Curcumin slows osteoarthritis progression and relieves osteoarthritis-associated pain symptoms in a post-traumatic osteoarthritis mouse model.. Arthritis Res Ther 2016 Jun 3;18(1):128.
- Veronesi F, Fini M, Giavaresi G, Ongaro A, De Mattei M, Pellati A, Setti S, Tschon M. Experimentally induced cartilage degeneration treated by pulsed electromagnetic field stimulation; an in vitro study on bovine cartilage.. BMC Musculoskelet Disord 2015 Oct 20;16:308.
- Natarajan V, Madhan B, Tiku ML. Intra-Articular Injections of Polyphenols Protect Articular Cartilage from Inflammation-Induced Degradation: Suggesting a Potential Role in Cartilage Therapeutics.. PLoS One 2015;10(6):e0127165.
- Hozhabri NS, Benson MD, Vu MD, Patel RH, Martinez RM, Nakhaie FN, Kim HK, Varanasi VG. Decreasing NF-u03baB expression enhances odontoblastic differentiation and collagen expression in dental pulp stem cells exposed to inflammatory cytokines.. PLoS One 2015;10(1):e0113334.
- Zhao R, Peng X, Li Q, Song W. Effects of phosphorylatable short peptide-conjugated chitosan-mediated IL-1Ra and igf-1 gene transfer on articular cartilage defects in rabbits.. PLoS One 2014;9(11):e112284.
- Appelboom T, Maes N, Albert A. A new curcuma extract (flexofytolu00ae) in osteoarthritis: results from a belgian real-life experience.. Open Rheumatol J 2014;8:77-81.
- Leong DJ, Choudhury M, Hirsh DM, Hardin JA, Cobelli NJ, Sun HB. Nutraceuticals: potential for chondroprotection and molecular targeting of osteoarthritis.. Int J Mol Sci 2013 Nov 21;14(11):23063-85.
- Henrotin Y, Priem F, Mobasheri A. Curcumin: a new paradigm and therapeutic opportunity for the treatment of osteoarthritis: curcumin for osteoarthritis management.. Springerplus 2013 Dec;2(1):56.
- Jabaut J, Ather JL, Taracanova A, Poynter ME, Ckless K. Mitochondria-targeted drugs enhance Nlrp3 inflammasome-dependent IL-1u03b2 secretion in association with alterations in cellular redox and energy status.. Free Radic Biol Med 2013 Jul;60:233-45.
- Mobasheri A. Intersection of inflammation and herbal medicine in the treatment of osteoarthritis.. Curr Rheumatol Rep 2012 Dec;14(6):604-16.
- Ather JL, Ckless K, Martin R, Foley KL, Suratt BT, Boyson JE, Fitzgerald KA, Flavell RA, Eisenbarth SC, Poynter ME. Serum amyloid A activates the NLRP3 inflammasome and promotes Th17 allergic asthma in mice.. J Immunol 2011 Jul 1;187(1):64-73.
- Shakibaei M, Mobasheri A, Buhrmann C. Curcumin synergizes with resveratrol to stimulate the MAPK signaling pathway in human articular chondrocytes in vitro.. Genes Nutr 2011 May;6(2):171-9.
- Clutterbuck AL, Smith JR, Allaway D, Harris P, Liddell S, Mobasheri A. High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation.. J Proteomics 2011 May 1;74(5):704-15.
- Wann AK, Mistry J, Blain EJ, Michael-Titus AT, Knight MM. Eicosapentaenoic acid and docosahexaenoic acid reduce interleukin-1u03b2-mediated cartilage degradation.. Arthritis Res Ther 2010;12(6):R207.
Secure Payments
Satisfaction Guarantee
We offer a 60-day guarantee to ensure you are completely satisfied.
Fast & Free Shipping
We provide fast and free shipping on all orders over $40.