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Home / Equine Research Bank / Vet Anim Sci / Interrelationship between reproductive hormones and acute ph...
Veterinary and animal science2021; 14; 100212; doi: 10.1016/j.vas.2021.100212

Interrelationship between reproductive hormones and acute phase proteins during estrous cycle and pregnancy in Spanish purebred broodmares.

Abstract: In some species, female steroid hormones modify the profile of acute phase proteins (APPs) during the estrous cycle and pregnancy, according to the ovulation, embryonic implantation and placental development; however, nowadays there's no experimental evidence for equine species. Objectives of this study were: to compare the serum amyloid A (SAA), haptoglobin (Hp) and C-reactive protein (CRP) concentrations between cyclic and pregnant mares, and to analyze the influence of estradiol-17β (E) during estrous cycle or estrone sulfate (E) during pregnancy, and progesterone (P) on these proteins to assess their potential role to identify the cyclicity or pregnancy in Spanish mares. Blood samples were taken from 20 Purebred Spanish mares on the day of ovulation (day 0), on days +5 and +16 post-ovulation, and then, monthly during the whole pregnancy. SAA, Hp and CRP did not change between day 0, +5 and +16 post-ovulation days. P concentrations were significantly higher on day +16 than on days +5 and 0; and E concentrations were significantly higher on day 0 than day +5. On the other hand, pregnancy was characterized by a progressive increase in the Hp, variable modifications of E and P concentrations, without changes in SAA and CRP. The absence of significant differences in the APPs between days 0, +5 and +16, suggested that these proteins cannot be used as biomarkers of diagnosis of heat or pregnancy in Spanish mares, at least early, since the Hp later increases during the gestation. Nevertheless, it is possible to use them for comparative purposes with other equine breeds, as supervisor instrument of health status in breeding females as diagnostic tools to monitor pregnancy's development and/or subclinical reproductive inflammations, that could lead to the early embryonic death.
© 2021 The Author(s).
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Publication Date: 2021-10-26 PubMed ID: 34765800PubMed Central: PMC8573188DOI: 10.1016/j.vas.2021.100212Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research is focused on exploring the relationship between reproductive hormones and acute phase proteins (APPs) in Spanish purebred mares during their estrous cycle and pregnancy, with an aim to identify potential biomarkers for fertility or pregnancy related conditions.

Objective of the Study

  • The primary goal of this study was to elucidate if APPs, specifically serum amyloid A (SAA), haptoglobin (Hp), and C-reactive protein (CRP) could serve as markers to indicate the reproductive status (cyclicity or pregnancy) of the mares. The researchers also aimed to understand how these proteins are influenced by reproductive hormones like estradiol-17β (E) during the estrous cycle and estrone sulfate (E) during pregnancy, and progesterone (P).

Methodology

  • The researchers drew blood samples from 20 Purebred Spanish mares at different critical points – on the day of ovulation (day 0), on days +5 and +16 post-ovulation, and then, monthly throughout the entire pregnancy.

Findings

  • The concentrations of SAA, Hp, and CRP showed no notable change between day 0, +5 and +16 post-ovulation.
  • Progesterone (P) levels were significantly higher on day +16 than on days +5 and 0, while estradiol-17β (E) levels were significantly higher on day 0 than on day +5.
  • During pregnancy, there was a marked increase in haptoglobin (Hp) levels, and variable modifications in E and P levels, however, no changes were observed in SAA and CRP levels.

Implications

  • Since there were no significant changes in the APPs levels at critical points of the estrous cycle, the study indicates that these proteins cannot be used as biomarkers to detect heat or early stages of pregnancy in Spanish mares.
  • However, the study does not negate the potential use of these proteins as a reference for comparative studies with other equine breeds, as an indicator of health status in breeding females, and as diagnostic tools to monitor pregnancy development and/or subclinical reproductive inflammations, which could potentially lead to early embryonic death.

Cite This Article

APA
Satué K, Calvo A, Muñoz A, Fazio E, Medica P. (2021). Interrelationship between reproductive hormones and acute phase proteins during estrous cycle and pregnancy in Spanish purebred broodmares. Vet Anim Sci, 14, 100212. https://doi.org/10.1016/j.vas.2021.100212

Publication

Veterinary and animal science
ISSN: 2451-943X
NlmUniqueID: 101694897
Country: Netherlands
Language: English
Volume: 14
Pages: 100212
PII: 100212

Researcher Affiliations

Satué, Katiuska
  • Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, CEU-Cardenal Herrera University, Avd. Seminari CEU, s/n, Valencia, 46113 Spain.
Calvo, Antonio
  • Department of Animal Medicine and Surgery, School of Veterinary and Experimental Sciences, Catholic University of Valencia San Vicente Mártir, Valencia, Spain.
Muñoz, Ana
  • Department of Animal Medicine and Surgery, School of Veterinary Medicine, University of Córdoba, Spain.
Fazio, Esterina
  • Department of Veterinary Sciences, Veterinary Physiology Unit, Messina University, Italy.
Medica, Pietro
  • Department of Veterinary Sciences, Veterinary Physiology Unit, Messina University, Italy.

Conflict of Interest Statement

None of the authors of the present article has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper.

References

This article includes 56 references
  1. Albay M.K., Karakurum M.C., Sahinduran S., Sezer K., Yildiz R., Buyukoglu T.. Selected serum biochemical parameters and acute phase protein levels in a herd of Saanen goats showing signs of pregnancy toxaemia. Veterinary Medical 2014;59:2336–2342.
  2. Belo L, Santos-Silva A, Rocha S, Caslake M, Cooney J, Pereira-Leite L, Quintanilha A, Rebelo I. Fluctuations in C-reactive protein concentration and neutrophil activation during normal human pregnancy.. Eur J Obstet Gynecol Reprod Biol 2005 Nov 1;123(1):46-51.
    pubmed: 16260340doi: 10.1016/j.ejogrb.2005.02.022google scholar: lookup
  3. Berkova N, Lemay A, Dresser DW, Fontaine JY, Kerizit J, Goupil S. Haptoglobin is present in human endometrium and shows elevated levels in the decidua during pregnancy.. Mol Hum Reprod 2001 Aug;7(8):747-54.
    pubmed: 11470862doi: 10.1093/molehr/7.8.747google scholar: lookup
  4. Bicho MC, Pereira da Silva A, Matos A, Silva RM, Bicho MD. Sex steroid hormones influence the risk for cervical cancer: modulation by haptoglobin genetic polymorphism.. Cancer Genet Cytogenet 2009 Jun;191(2):85-9.
    pubmed: 19446743doi: 10.1016/j.cancergencyto.2009.02.005google scholar: lookup
  5. Bowen JM, Chamley L, Mitchell MD, Keelan JA. Cytokines of the placenta and extra-placental membranes: biosynthesis, secretion and roles in establishment of pregnancy in women.. Placenta 2002 Apr;23(4):239-56.
    pubmed: 11969335doi: 10.1053/plac.2001.0781google scholar: lookup
  6. Canisso IF, Ball BA, Cray C, Williams NM, Scoggin KE, Davolli GM, Squires EL, Troedsson MH. Serum amyloid A and haptoglobin concentrations are increased in plasma of mares with ascending placentitis in the absence of changes in peripheral leukocyte counts or fibrinogen concentration.. Am J Reprod Immunol 2014 Oct;72(4):376-85.
    pubmed: 24916762doi: 10.1111/aji.12278google scholar: lookup
  7. Capobianco G, de Muro P, Cherchi GM, Formato M, Lepedda AJ, Cigliano A, Zinellu E, Dessole F, Gordini L, Dessole S. Plasma levels of C-reactive protein, leptin and glycosaminoglycans during spontaneous menstrual cycle: differences between ovulatory and anovulatory cycles.. Arch Gynecol Obstet 2010 Aug;282(2):207-13.
    pubmed: 20306065doi: 10.1007/s00404-010-1432-2google scholar: lookup
  8. Chakrabarti S, Lekontseva O, Davidge ST. Estrogen is a modulator of vascular inflammation.. IUBMB Life 2008 Jun;60(6):376-82.
    pubmed: 18409173doi: 10.1002/iub.48google scholar: lookup
  9. Christoffersen M, Woodward E, Bojesen AM, Jacobsen S, Petersen MR, Troedsson MH, Lehn-Jensen H. Inflammatory responses to induced infectious endometritis in mares resistant or susceptible to persistent endometritis.. BMC Vet Res 2012 Mar 29;8:41.
    pmc: PMC3368729pubmed: 22458733doi: 10.1186/1746-6148-8-41google scholar: lookup
  10. Clancy KB, Baerwald AR, Pierson RA. Systemic inflammation is associated with ovarian follicular dynamics during the human menstrual cycle.. PLoS One 2013;8(5):e64807.
    pmc: PMC3661529pubmed: 23717660doi: 10.1371/journal.pone.0064807google scholar: lookup
  11. Coutinho da Silva MA, Canisso IF, MacPherson ML, Johnson AE, Divers TJ. Serum amyloid A concentration in healthy periparturient mares and mares with ascending placentitis.. Equine Vet J 2013 Sep;45(5):619-24.
    pubmed: 23432049doi: 10.1111/evj.12034google scholar: lookup
  12. Cray C., Zaias J., Altman N.H.. Acute phase response in animals: A review. Comparative Medicine 2009;59:10.
    pubmed: 0
  13. Czopowicz M, Szaluś-Jordanow O, Mickiewicz M, Witkowski L, Markowska-Daniel I, Stefaniak T, Reczyńska D, Bagnicka E, Kaba J. Acute-phase proteins in pregnant goats: a longitudinal study.. J Vet Diagn Invest 2017 Nov;29(6):814-819.
    pubmed: 28643602doi: 10.1177/1040638717714295google scholar: lookup
  14. Eckersall PD, Harvey MJ, Ferguson JM, Renton JP, Nickson DA, Boyd JS. Acute phase proteins in canine pregnancy (Canis familiaris).. J Reprod Fertil Suppl 1993;47:159-64.
    pubmed: 8229922
  15. Flaminio MJ, Antczak DF. Inhibition of lymphocyte proliferation and activation: a mechanism used by equine invasive trophoblast to escape the maternal immune response.. Placenta 2005 Feb-Mar;26(2-3):148-59.
    pubmed: 15708116doi: 10.1016/j.placenta.2004.05.008google scholar: lookup
  16. Gaskins AJ, Wilchesky M, Mumford SL, Whitcomb BW, Browne RW, Wactawski-Wende J, Perkins NJ, Schisterman EF. Endogenous reproductive hormones and C-reactive protein across the menstrual cycle: the BioCycle Study.. Am J Epidemiol 2012 Mar 1;175(5):423-31.
    pmc: PMC3282877pubmed: 22306563doi: 10.1093/aje/kwr343google scholar: lookup
  17. Gatzka C, Bremerich D, Kaufmann M, Ahr A. [Isolated decrease of haptoglobin during pregnancy: diagnosis by chance or pathological? ].. Zentralbl Gynakol 2002 Feb;124(2):120-2.
    pubmed: 11935498doi: 10.1055/s-2002-24234google scholar: lookup
  18. Gruys E, Toussaint MJ, Niewold TA, Koopmans SJ. Acute phase reaction and acute phase proteins.. J Zhejiang Univ Sci B 2005 Nov;6(11):1045-56.
    pmc: PMC1390650pubmed: 16252337doi: 10.1631/jzus.2005.b1045google scholar: lookup
  19. Haram K, Augensen K, Elsayed S. Serum protein pattern in normal pregnancy with special reference to acute-phase reactants.. Br J Obstet Gynaecol 1983 Feb;90(2):139-45.
    pubmed: 6600623doi: 10.1111/j.1471-0528.1983.tb08898.xgoogle scholar: lookup
  20. Heikkinen J, Möttönen M, Komi J, Alanen A, Lassila O. Phenotypic characterization of human decidual macrophages.. Clin Exp Immunol 2003 Mar;131(3):498-505.
    pmc: PMC1808648pubmed: 12605704doi: 10.1046/j.1365-2249.2003.02092.xgoogle scholar: lookup
  21. Herrler A, Krusche CA, Müller-Schöttle F, Beier HM. Haptoglobin expression and release by rabbit oviduct and endometrium, its localization in blastocyst extra-embryonic matrix and fluid during preimplantation time.. Hum Reprod 2004 Dec;19(12):2730-7.
    pubmed: 15388678doi: 10.1093/humrep/deh517google scholar: lookup
  22. Jacobsen S., Andersen P.H.. The acute phase protein serum amyloid A (SAA) as a marker of inflammation in horses. Scandinavian Journal of Immunology 2007;19:38–46.
  23. Jacobsen S, Vinther AM, Kjelgaard-Hansen M, Nielsen LN. Validation of an equine serum amyloid A assay with an unusually broad working range.. BMC Vet Res 2019 Dec 19;15(1):462.
    pmc: PMC6923866pubmed: 31856804doi: 10.1186/s12917-019-2211-3google scholar: lookup
  24. Kosaka K, Fujiwara H, Tatsumi K, Yoshioka S, Sato Y, Egawa H, Higuchi T, Nakayama T, Ueda M, Maeda M, Fujii S. Human chorionic gonadotropin (HCG) activates monocytes to produce interleukin-8 via a different pathway from luteinizing hormone/HCG receptor system.. J Clin Endocrinol Metab 2002 Nov;87(11):5199-208.
    pubmed: 12414893doi: 10.1210/jc.2002-020341google scholar: lookup
  25. Kovacevic A, Hammer A, Sundl M, Pfister B, Hrzenjak A, Ray A, Ray BK, Sattler W, Malle E. Expression of serum amyloid A transcripts in human trophoblast and fetal-derived trophoblast-like choriocarcinoma cells.. FEBS Lett 2006 Jan 9;580(1):161-7.
    pubmed: 16343490doi: 10.1016/j.febslet.2005.11.067google scholar: lookup
  26. Krakowski L, Bartoszek P, Krakowska I, Olcha P, Piech T, Stachurska A, Brodzki P. Serum amyloid A protein (SAA), haptoglobin (Hp) and selected hematological and biochemical parameters in wild mares before and after parturition.. Pol J Vet Sci 2017 Mar 1;20(2):299-305.
    pubmed: 28865214doi: 10.1515/pjvs-2017-0036google scholar: lookup
  27. Krakowski L, Brodzki P, Krakowska I, Opielak G, Marczuk J, Piech T. The Level of Prolactin, Serum Amyloid A, and Selected Biochemical Markers in Mares Before and After Parturition and Foal Heat.. J Equine Vet Sci 2020 Jan;84:102854.
    pubmed: 31864459doi: 10.1016/j.jevs.2019.102854google scholar: lookup
  28. Krakowski L, Krawczyk CH, Kostro K, Stefaniak T, Novotny F, Obara J. Serum levels of acute phase proteins: SAA, Hp and progesterone (P4) in mares with early embryonic death.. Reprod Domest Anim 2011 Aug;46(4):624-9.
    pubmed: 21241377doi: 10.1111/j.1439-0531.2010.01715.xgoogle scholar: lookup
  29. Krakowski L., Zdzisinska B.. Selected cytokines and acute phase proteins in heifers duringthe ovarian cycle course and in different pregnancy periods. Bulletin of the Veterinary Institute in Pulawy 2007;51:31–36.
  30. Kristensen K, Wide-Swensson D, Lindstrom V, Schmidt C, Grubb A, Strevens H. Serum amyloid a protein and C-reactive protein in normal pregnancy and preeclampsia.. Gynecol Obstet Invest 2009;67(4):275-80.
    pubmed: 19390201doi: 10.1159/000214081google scholar: lookup
  31. Kuribayashi T, Shimada T, Matsumoto M, Kawato K, Honjyo T, Fukuyama M, Yamamoto Y, Yamamoto S. Determination of serum C-reactive protein (CRP) in healthy beagle dogs of various ages and pregnant beagle dogs.. Exp Anim 2003 Oct;52(5):387-90.
    pubmed: 14625403doi: 10.1538/expanim.52.387google scholar: lookup
  32. Kuru M., Merhan O., Kaya S., Oral H., Kukurt A.. The effect of short term progesterone-releasing intravaginal device treatment on acute inflammation markers for Holstein heifers. Rev Med Ve 2015;166:336–340.
  33. Larsson A, Palm M, Hansson LO, Basu S, Axelsson O. Reference values for alpha1-acid glycoprotein, alpha1-antitrypsin, albumin, haptoglobin, C-reactive protein, IgA, IgG and IgM during pregnancy.. Acta Obstet Gynecol Scand 2008;87(10):1084-8.
    pubmed: 18792844doi: 10.1080/00016340802428146google scholar: lookup
  34. Nunokawa Y, Fujinaga T, Taira T, Okumura M, Yamashita K, Tsunoda N, Hagio M. Evaluation of serum amyloid A protein as an acute-phase reactive protein in horses.. J Vet Med Sci 1993 Dec;55(6):1011-6.
    pubmed: 7509640doi: 10.1292/jvms.55.1011google scholar: lookup
  35. Orvieto R, Chen R, Ashkenazi J, Ben-Haroush A, Bar J, Fisch B. C-reactive protein levels in patients undergoing controlled ovarian hyperstimulation for IVF cycle.. Hum Reprod 2004 Feb;19(2):357-9.
    pubmed: 14747180doi: 10.1093/humrep/deh089google scholar: lookup
  36. Osman I, Young A, Ledingham MA, Thomson AJ, Jordan F, Greer IA, Norman JE. Leukocyte density and pro-inflammatory cytokine expression in human fetal membranes, decidua, cervix and myometrium before and during labour at term.. Mol Hum Reprod 2003 Jan;9(1):41-5.
    pubmed: 12529419doi: 10.1093/molehr/gag001google scholar: lookup
  37. Sacks GP, Seyani L, Lavery S, Trew G. Maternal C-reactive protein levels are raised at 4 weeks gestation.. Hum Reprod 2004 Apr;19(4):1025-30.
    pubmed: 14990546doi: 10.1093/humrep/deh179google scholar: lookup
  38. Sacks GP, Studena K, Sargent K, Redman CW. Normal pregnancy and preeclampsia both produce inflammatory changes in peripheral blood leukocytes akin to those of sepsis.. Am J Obstet Gynecol 1998 Jul;179(1):80-6.
    pubmed: 9704769doi: 10.1016/s0002-9378(98)70254-6google scholar: lookup
  39. Samimi AS, Aghamiri SM, Babaei H, Heidarabadypor R. Changes of acute-phase proteins during different phases of the estrous cycle in Ovsynch-synchronized Holstein cows.. Iran J Vet Res 2020 Summer;21(3):226-229.
    pmc: PMC7608038pubmed: 33178302
  40. Sandri S, Urban Borbely A, Fernandes I, de Oliveira EM, Knebel FH, Ruano R, Zugaib M, Filippin-Monteiro F, Bevilacqua E, Campa A. Serum amyloid A in the placenta and its role in trophoblast invasion.. PLoS One 2014;9(3):e90881.
    pmc: PMC3948705pubmed: 24614130doi: 10.1371/journal.pone.0090881google scholar: lookup
  41. Satoh M, Fujinaga T, Okumura M, Hagio M. Sandwich enzyme-linked immunosorbent assay for quantitative measurement of serum amyloid A protein in horses.. Am J Vet Res 1995 Oct;56(10):1286-91.
    pubmed: 8928944
  42. Satué K., Calvo A., Gardón J.C.. Factors influencing serum amyloid type A (SAA)concentrations in horses. Open Journal of Veterinary Medicine 2013;3:58–66.
  43. Satué K, Marcilla M, Medica P, Ferlazzo A, Fazio E. Sequential concentrations of placental growth factor and haptoglobin, and their relation to oestrone sulphate and progesterone in pregnant Spanish Purebred mare.. Theriogenology 2018 Jul 15;115:77-83.
    pubmed: 29730538doi: 10.1016/j.theriogenology.2018.04.033google scholar: lookup
  44. Sciorsci RL, Galgano M, Mutinati M, Rizzo A. Oxidative state in the estrus cycle of the buffaloes: a preliminary study.. Trop Anim Health Prod 2020 May;52(3):1331-1334.
    pubmed: 31768890doi: 10.1007/s11250-019-02133-4google scholar: lookup
  45. Sikora M, Król J, Nowak M, Stefaniak T, Aubertsson G, Kozdrowski R. The usefulness of uterine lavage and acute phase protein levels as a diagnostic tool for subclinical endometritis in Icelandic mares.. Acta Vet Scand 2016 Sep 7;58(1):50.
    pmc: PMC5015198pubmed: 27604098doi: 10.1186/s13028-016-0233-4google scholar: lookup
  46. Sorrells AD, Eicher SD, Harris MJ, Pajor EA, Richert BT. Periparturient cortisol, acute phase cytokine, and acute phase protein profiles of gilts housed in groups or stalls during gestation.. J Anim Sci 2007 Jul;85(7):1750-7.
    pubmed: 17468426doi: 10.2527/jas.2007-0025google scholar: lookup
  47. Straub RH. The complex role of estrogens in inflammation.. Endocr Rev 2007 Aug;28(5):521-74.
    pubmed: 17640948doi: 10.1210/er.2007-0001google scholar: lookup
  48. Taira T, Fujinaga T, Okumura M, Yamashita K, Tsunoda N, Mizuno S. Equine haptoglobin: isolation, characterization, and the effects of ageing, delivery and inflammation on its serum concentration.. J Vet Med Sci 1992 Jun;54(3):435-42.
    pubmed: 1643167doi: 10.1292/jvms.54.435google scholar: lookup
  49. Tuppits U, Orro T, Einarsson S, Kask K, Kavak A. Influence of the uterine inflammatory response after insemination with frozen-thawed semen on serum concentrations of acute phase proteins in mares.. Anim Reprod Sci 2014 May;146(3-4):182-6.
    pubmed: 24636940doi: 10.1016/j.anireprosci.2014.02.007google scholar: lookup
  50. Ulutas PA, Musal B, Kiral F, Bildik A. Acute phase protein levels in pregnancy and oestrus cycle in bitches.. Res Vet Sci 2009 Jun;86(3):373-6.
    pubmed: 18995874doi: 10.1016/j.rvsc.2008.09.001google scholar: lookup
  51. Valtonen P, Punnonen K, Saarelainen H, Heiskanen N, Raitakari OT, Viikari JS, Lyyra-Laitinen T, Laitinen T, Heinonen S. Maternal serum ADMA is not associated with proinflammatory cytokines or C-reactive protein during normal pregnancy.. Cytokine 2009 May;46(2):216-21.
    pubmed: 19268605doi: 10.1016/j.cyto.2009.01.011google scholar: lookup
  52. Vannucchi CI, Mirandola RM, Oliveira CM. Acute-phase protein profile during gestation and diestrous: proposal for an early pregnancy test in bitches.. Anim Reprod Sci 2002 Nov 15;74(1-2):87-99.
    pubmed: 12379378doi: 10.1016/s0378-4320(02)00160-4google scholar: lookup
  53. Wander K, Brindle E, O'Connor KA. C-reactive protein across the menstrual cycle.. Am J Phys Anthropol 2008 Jun;136(2):138-46.
    pubmed: 18257023doi: 10.1002/ajpa.20785google scholar: lookup
  54. Wolf CA, Maslchitzky E, Gregory RM, Jobim MI, Mattos RC. Effect of corticotherapy on proteomics of endometrial fluid from mares susceptible to persistent postbreeding endometritis.. Theriogenology 2012 Apr 15;77(7):1351-9.
    pubmed: 22225686doi: 10.1016/j.theriogenology.2011.10.042google scholar: lookup
  55. Xu P, Alfaidy N, Challis JR. Expression of matrix metalloproteinase (MMP)-2 and MMP-9 in human placenta and fetal membranes in relation to preterm and term labor.. J Clin Endocrinol Metab 2002 Mar;87(3):1353-61.
    pubmed: 11889208doi: 10.1210/jcem.87.3.8320google scholar: lookup
  56. Yamashita K, Fujinaga T, Okumura M, Takiguchi M, Tsunoda N, Mizuno S. Serum C-reactive protein (CRP) in horses: the effect of aging, sex, delivery and inflammations on its concentration.. J Vet Med Sci 1991 Dec;53(6):1019-24.
    pubmed: 1790210doi: 10.1292/jvms.53.1019google scholar: lookup

Citations

This article has been cited 3 times.
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