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Home / Equine Research Bank / Biomolecules / Investigating the Suitability of Mare’s Milk-Derived E...
Biomolecules2024; 14(10); 1247; doi: 10.3390/biom14101247

Investigating the Suitability of Mare’s Milk-Derived Exosomes as Potential Drug Carriers.

Abstract: Exosomes are cell-derived, membrane-surrounded particles that deliver bioactive molecules to various cells. Due to their small size, low immunogenicity, extended blood circulation, and involvement in cellular communication, they hold potential as effective drug carriers. Exosomes are present in various biological fluids, including mare's milk, a traditional drink in Central Asia. This study aims to compare exosome isolation methodologies and determine the stability of mare's milk-derived exosomes as potential therapeutic carriers. Three extraction methods-immunoprecipitation, size exclusion chromatography, and total exosome isolation-were compared in terms of exosome characteristics, purity, and content. The isolated exosomes were then loaded with quercetin, and their ability to increase its bioavailability was tested in vitro and in vivo. Total exosome isolation was identified as the most efficient method for producing high-quality exosomes. These exosomes were loaded with quercetin and compared to free quercetin and exosomes alone. Exosomes loaded with 80 µM quercetin significantly restored β-galactosidase activity and cellular viability in doxorubicin-treated cells, exhibiting similar potency to 160 µM free quercetin. In aged model animals, treatment with quercetin-loaded exosomes resulted in significantly less acute and subacute damage to the myocardium, kidneys, and liver compared to untreated control animals. This study provides a proof-of-concept that mare's milk-derived exosomes can be effectively absorbed by cells and animal tissues, supporting their potential use as drug carriers.
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Publication Date: 2024-10-01 PubMed ID: 39456180PubMed Central: PMC11506534DOI: 10.3390/biom14101247Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper investigates the effectiveness of mare’s milk-derived exosomes as potential drug carriers. It establishes that exosomes, when loaded with the flavonoid quercetin, can efficiently restore cell vitality, enhance medication bioavailability, and lessen tissue damage in an aged model animal.

Research Objective and Methods

  • This study aims to assess the efficacy of mare’s milk-derived exosomes as potential therapeutic carriers.
  • Three isolation methods, namely, immunoprecipitation, size exclusion chromatography, and total exosome isolation were utilized and compared for their efficiency in producing high-quality exosomes.
  • The isolated exosomes were loaded with quercetin, a plant pigment with powerful antioxidant and anti-inflammatory effects, to test their medicinal bioavailability.
  • The process was tested both in vitro (in a controlled environment outside a living organism) and in vivo (within a living organism).

Key Findings

  • Total exosome isolation emerged as the most efficient method for producing high-quality exosomes from mare’s milk.
  • Exosomes loaded with 80 µM quercetin significantly enhanced β-galactosidase activity and cellular vitality in doxorubicin-treated cells, with an efficacy similar to that of 160 µM free quercetin.
  • In an aged model animal, treatment with quercetin-loaded exosomes resulted in notably less acute and subacute damage to the myocardium, kidneys, and liver compared to untreated control animals.

Implications

  • The study provides experimental proof that mare’s milk-derived exosomes hold promise as drug carriers due to their effective absorption by cells and animal tissues.
  • This discovery could potentially revolutionize drug delivery as it enhances the bioavailability of drugs while reducing systemic damage to the organs.
  • However, further research is needed to validate these findings in humans and explore other potential therapeutic applications of exosome-derived drug delivery.

Cite This Article

APA
Sergazy S, Zhetkenev S, Shulgau Z, Chulenbayeva L, Kamyshanskiy Y, Nurgaziyev M, Nurgozhina A, Mukhanbetzhanova Z, Berikkhanova K, Gulyayev A, Aljofan M. (2024). Investigating the Suitability of Mare’s Milk-Derived Exosomes as Potential Drug Carriers. Biomolecules, 14(10), 1247. https://doi.org/10.3390/biom14101247

Publication

Biomolecules
ISSN: 2218-273X
NlmUniqueID: 101596414
Country: Switzerland
Language: English
Volume: 14
Issue: 10
PII: 1247

Researcher Affiliations

Sergazy, Shynggys
  • National Laboratory Astana, Private Institution, Nazarbayev University, Astana 010000, Kazakhstan.
Zhetkenev, Sanzhar
  • National Laboratory Astana, Private Institution, Nazarbayev University, Astana 010000, Kazakhstan.
Shulgau, Zarina
  • National Laboratory Astana, Private Institution, Nazarbayev University, Astana 010000, Kazakhstan.
Chulenbayeva, Laura
  • National Laboratory Astana, Private Institution, Nazarbayev University, Astana 010000, Kazakhstan.
Kamyshanskiy, Yevgeniy
  • Laboratory and Pathological Diagnostic Unit, Karaganda State Medical University, Karaganda 100000, Kazakhstan.
Nurgaziyev, Madiyar
  • National Laboratory Astana, Private Institution, Nazarbayev University, Astana 010000, Kazakhstan.
Nurgozhina, Ayaulym
  • National Laboratory Astana, Private Institution, Nazarbayev University, Astana 010000, Kazakhstan.
Mukhanbetzhanova, Zhanel
  • National Laboratory Astana, Private Institution, Nazarbayev University, Astana 010000, Kazakhstan.
Berikkhanova, Kulzhan
  • National Laboratory Astana, Private Institution, Nazarbayev University, Astana 010000, Kazakhstan.
Gulyayev, Alexander
  • National Laboratory Astana, Private Institution, Nazarbayev University, Astana 010000, Kazakhstan.
Aljofan, Mohamad
  • National Laboratory Astana, Private Institution, Nazarbayev University, Astana 010000, Kazakhstan.
  • Department of Biomedical Science, Nazarbayev University School of Medicine, Astana 020000, Kazakhstan.

MeSH Terms

  • Animals
  • Exosomes / metabolism
  • Milk / chemistry
  • Milk / metabolism
  • Quercetin / pharmacology
  • Horses
  • Drug Carriers / chemistry
  • Doxorubicin / pharmacology
  • Humans
  • Female
  • Cell Survival / drug effects
  • Mice

Grant Funding

  • AP13067844 / Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan
  • OPCRP2021006 / Nazarbayev University Collaborative Research Program 2021-2023

Conflict of Interest Statement

The authors declare no conflicts of interest.

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