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Veterinary research communications2010; 34(8); 677-689; doi: 10.1007/s11259-010-9439-5

Investigation of selected biochemical indicators of Equine Rhabdomyolysis in Arabian horses: pro-inflammatory cytokines and oxidative stress markers.

Abstract: A total of 30 horses were divided into two groups, one served as a control whereas other was rhabdomyolysis diseased horses. After blood collection, the resulted sera were used for estimation of the activities of creatin kinase (CK), aspartate transaminase (AST), lactate dehydrogenase (LDH), lactic acid, triacylglycerol (TAG), glucose, total protein, albumin, globulin, urea, creatinine, Triiodothyronine (T(3)), calcium, sodium, potassium, phosphorus, chloride, vitamin E, interleukin-6 (IL-6) and tumor necrosis-α (TNF-α). In addition, whole blood was used for determination of selenium, reduced glutathione (G-SH) and prostaglandin F2-α (PGF2α). The erythrocyte hemolysates were used for the determination of the activities of super oxide dismutase (SOD), catalase (CAT), total antioxidant capacity (TAC), nitric oxide (NO) and malondialdehyde (MDA). The present findings revealed a significant (p ≤ 0.05) increase in the values of CK, AST, LDH, glucose, lactate, TAG, urea, creatinine, phosphorus, MDA, TNF- α, IL6 and PGF2- α in diseased horses when compared with the control. Furthermore, the values of calcium, SOD, CAT, TAC, NO and GSH in diseased horses were significantly (p ≤ 0.05) lower than the control. The other examined parameters were not statistically significant. In conclusion, the examined pro-inflammatory cytokines were useful biomarkers for the diagnosis of Equine rhabdomyolysis (ER) in Arabian horses beside the old examined biomarkers. In the future, efforts should be made to confirm this in other breed. If this could be achieved, it would open up new perspectives in research fields dealing with ER.
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Publication Date: 2010-09-10 PubMed ID: 20830520DOI: 10.1007/s11259-010-9439-5Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the biochemical indicators, particularly pro-inflammatory cytokines and oxidative stress markers, associated with Equine Rhabdomyolysis in Arabian horses.

Research Method

  • In this study, 30 horses were organised into two groups. One functioned as a control group, and the other consisted of horses afflicted with rhabdomyolysis.
  • The researchers collected blood from these horses and used the serum for the examination of various markers.
  • These markers included creatin kinase (CK), aspartate transaminase (AST), lactate dehydrogenase (LDH), lactic acid, triacylglycerol (TAG), glucose, total protein, albumin, globulin, urea, creatinine, Triiodothyronine (T(3)), calcium, sodium, potassium, phosphorus, chloride, vitamin E, interleukin-6 (IL-6) and tumor necrosis-α (TNF-α).
  • The whole blood was used for the determination of selenium, reduced glutathione (G-SH), and prostaglandin F2-α (PGF2α).
  • Lastly, erythrocyte hemolysates (red blood cell breakdown products) were studied for the activities of super oxide dismutase (SOD), catalase (CAT), total antioxidant capacity (TAC), nitric oxide (NO), and malondialdehyde (MDA).

Research Findings

  • Rhabdomyolysis diseased horses showed a significant increase in certain markers, including CK, AST, LDH, glucose, lactate, TAG, urea, creatinine, phosphorus, MDA, TNF- α, IL6 and PGF2- α.
  • The values of calcium, SOD, CAT, TAC, NO and GSH were significantly lower in diseased horses than the control.
  • Many other examined parameters did not show statistical significance.

Conclusion and Implications

  • The research concluded that the investigated pro-inflammatory cytokines could serve as useful biomarkers for the diagnosis of Equine Rhabdomyolysis in Arabian horses, along with long-established biomarkers.
  • These results advocate for more studies to be carried out on different breed types to ascertain if these biomarkers hold true.
  • If these biomarkers are found to be consistent across various breeds, they could be beneficially utilized in research fields dealing with Equine Rhabdomyolysis, providing new insights and possibly paving the way for fresh therapeutic interventions.

Cite This Article

APA
El-Deeb WM, El-Bahr SM. (2010). Investigation of selected biochemical indicators of Equine Rhabdomyolysis in Arabian horses: pro-inflammatory cytokines and oxidative stress markers. Vet Res Commun, 34(8), 677-689. https://doi.org/10.1007/s11259-010-9439-5

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 34
Issue: 8
Pages: 677-689

Researcher Affiliations

El-Deeb, Wael Mohamed
  • Department of clinical studies, College of Veterinary Medicine and animal Resources, King Faisal University, P.O. Box: 1757, Al-Ahsa, 31982, Saudi Arabia. drwaeleldeeb@yahoo.com
El-Bahr, Sabry M

    MeSH Terms

    • Animals
    • Biomarkers / blood
    • Blood Chemical Analysis / veterinary
    • Cytokines / blood
    • Cytokines / immunology
    • Horse Diseases / blood
    • Horse Diseases / immunology
    • Horses
    • Male
    • Rhabdomyolysis / blood
    • Rhabdomyolysis / immunology
    • Rhabdomyolysis / veterinary

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