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Isolation, detection and identification of some alkaloids or alkaloid-like substances in biological specimens from horses with special reference to doping.
Abstract: The isolation, detection and identification of twelve alkaloids or alkaloid-like substances from aqueous solutions are described. The best extraction was obtained with chloroform, and the detection and identification was performed by thin-layer chromatography.
These extraction and chromatography procedures were applied to the isolation and detection of the same alkaloids from biological materials after their injection and passage through the bodies of horses. Samples of saliva, plasma and urine were examined at different times after the injection. Urine seemed to be the most reliable biological fluid for the detection of these substances. The method fully satisfies the requirements for the rapid determination of these substances in doped race horses and even in human athletes.
Publication Date: 1968-06-04 PubMed ID: 5657925DOI: 10.1016/s0021-9673(01)82380-3Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study involves the identification and detection of twelve specific alkaloids in horse biological samples, with implications for anti-doping detection in horse racing.
Research Goal
The research aimed to develop a method of isolating, detecting, and identifying certain alkaloids or alkaloid-like substances from biological samples. This method is important in understanding substance use in horse racing and potentially in athletic competitions.
Methodology
- The researchers began by isolating these substances from aqueous solutions. The most successful method for extraction was found to be with the use of chloroform.
- They then identified and detected the substances using thin-layer chromatography, a technique widely used for separating and identifying compounds.
- The same procedures were then applied to horse biological material like saliva, plasma, and urine. These materials were collected at varied intervals after the substances were injected into the horses’ body.
Findings
- The results showed that urine was the most reliable fluid for detecting these substances. This could be due to how the substances are metabolized and excreted.
- It was concluded that this method meets the required standards for rapid determination of these substances in race horses suspected of doping. The findings could also be relevant for detecting substance abuse in human athletes as well.
Implications
- This research may have significant implications for anti-doping efforts in horse racing. If the method can detect alkaloids quickly and reliably, it can help ensure a level playing field by detecting illicit substance use.
- Although the study focused on horses, there may also be potential applications in anti-doping tests for human athletes, as similar biological processes are involved.
Overall, the study contributes to the field of doping detection, providing a reliable method for identifying specific alkaloids or alkaloid-like substances from biological samples.
Cite This Article
APA
Debackere M, Laruelle L.
(1968).
Isolation, detection and identification of some alkaloids or alkaloid-like substances in biological specimens from horses with special reference to doping.
J Chromatogr, 35(2), 234-247.
https://doi.org/10.1016/s0021-9673(01)82380-3 Publication
Researcher Affiliations
MeSH Terms
- Alkaloids / analysis
- Alkaloids / blood
- Alkaloids / urine
- Animals
- Chromatography, Thin Layer
- Female
- Horses
- Saliva / analysis
- Sports
- Stimulation, Chemical
Citations
This article has been cited 3 times.- Vidic E. [Detection and decomposition time of drugs in blood specimens]. Arch Toxikol 1970;27(1):19-39.
- Conine F, Paul J. A thin-layer chromatographic procedure for the separation of aspirin, cocaine, caffeine, codeine, heroin, 6-monoacetyl morphine, and morphine. Mikrochim Acta 1974;(3):443-8.
- Delbeke FT, Debackere M. Excretion and metabolism of nikethamide in the horse. Br J Sports Med 1976 Oct;10(3):116-23.
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