Isolation of equine papillomavirus type 1 from racing horse in Japan.
Abstract: Although many studies have been conducted worldwide for Equus caballus papillomavirus (EcPV), limited information is available on the virus in Japan. We recently collected one classical viral papillomatosis sample (E150904) from a racing horse in Japan. Papillomavirus infection was confirmed by histopathology, immunohistochemistry and PCR assays, and the sample was diagnosed as epithelial papilloma. Full-length genome of the virus was cloned and sequenced. It was 7,613 bp in length and had the same genome organization with reported EcPV-1. Moreover, phylogenetic analysis based on L1 gene revealed that the infection was caused by a variant of EcPV-1. This is the first report of EcPV infection in Japan, and would further contribute to the molecular epidemiological and pathological studies for EcPV.
Publication Date: 2017-10-08 PubMed ID: 28993549PubMed Central: PMC5745171DOI: 10.1292/jvms.17-0322Google Scholar: Lookup
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- Journal Article
Summary
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This research centres on the successful isolation and analysis of a type of papillomavirus in horses (EcPV-1) in Japan. This is the country’s first known occurrence of this virus, unveiling new information that can support future studies on its epidemiology and pathology.
Context and Objective
- This research was motivated by the limited understanding of the Equus caballus papillomavirus (EcPV) in Japan, despite global studies on the virus. This motivated the scientists to study EcPV locally.
- An objective of the research was to isolate and analyze the viral genome, thus deepening the understanding of EcPV in Japan and contributing to molecular epidemiological and pathological studies for the virus.
Methodology
- The authors took a sample (E150904) from a racing horse showing symptoms of classical viral papillomatosis in Japan.
- They conducted a series of tests to confirm the presence of the papillomavirus infection. These tests included histopathology (study of the microscopic structure of tissues), immunohistochemistry (localization of antigens or proteins in tissues), and PCR (Polymerase Chain Reaction) assays, a technique used to amplify specific sections of DNA.
- Upon confirmation of the papillomavirus infection, the sample was diagnosed as epithelial papilloma. This is a benign tumorous growth that originates from the epithelial tissue.
Findings
- The scientists were able to clone and sequence the full-length genome of the virus, an achievement that paves the path for deeper analysis and understanding of the virus.
- The genome was found to be 7,613 base pairs in length, similar to previously reported EcPV-1 viruses. This similarity in length supports the hypothesis that they share the same genome organization.
- A phylogenetic analysis based on the L1 gene (a significant gene in papillomaviruses often used for comparison and classification) revealed that the infection was caused by a variant of EcPV-1.
Impact
- This study presents the first report of EcPV infection in Japan. This brings new light to the local presence and variation of the virus, building a foundation for more culturally, regionally, and genetically specific studies on EcPV in Japan.
- The completed sequencing of the virus genome in this study highlights a critical step towards the development of advanced strategies for the management and treatment of this virus. This breakthrough also opens avenues for broader and deeper research into its molecular epidemiology and pathology.
Cite This Article
APA
Dong J, Zhu W, Yamashita N, Chambers JK, Uchida K, Kuwano A, Haga T.
(2017).
Isolation of equine papillomavirus type 1 from racing horse in Japan.
J Vet Med Sci, 79(12), 1957-1959.
https://doi.org/10.1292/jvms.17-0322 Publication
Researcher Affiliations
- Shandong Vocational Animal Science and Veterinary College, 88, Shenglidong Street, Weifang 261061, China.
- Division of Infection Control and Disease Prevention, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan.
- Qinghai University and Tsinghua University Three Rivers Source Research Institute, Qinghai University, 25, Ningda Road, Xining 810016, China.
- Shandong Vocational Animal Science and Veterinary College, 88, Shenglidong Street, Weifang 261061, China.
- Division of Infection Control and Disease Prevention, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan.
- Qinghai University and Tsinghua University Three Rivers Source Research Institute, Qinghai University, 25, Ningda Road, Xining 810016, China.
- Division of Infection Control and Disease Prevention, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan.
- Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan.
- Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan.
- Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
- Current address: Japan Farriery Association, JRA Shimbashi Bunkan Bldg., 4-5-4 Shimbashi, Minato-ku, Tokyo 105-0004, Japan.
- Division of Infection Control and Disease Prevention, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan.
MeSH Terms
- Animals
- Genome, Viral
- Horse Diseases / virology
- Horses
- Japan / epidemiology
- Male
- Papilloma / veterinary
- Papilloma / virology
- Papillomaviridae / genetics
- Papillomaviridae / isolation & purification
- Papillomavirus Infections / veterinary
- Phylogeny
- Polymerase Chain Reaction / veterinary
- Sequence Analysis, DNA
References
This article includes 9 references
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Citations
This article has been cited 5 times.- Turco S, Gabbianelli F, Mavian CN, Pietrucci D, De Paolis L, Gialletti R, Mechelli L, De Ciucis CG, Cappelli K, Dell'Anno F, Mecocci S, Donato GG, Nervo T, Fruscione F, Crescio MI, Ghelardi A, Chillemi G, Razzuoli E. Genetic Characterization of a Novel Equus caballus Papillomavirus Isolated from a Thoroughbred Mare. Viruses 2023 Feb 28;15(3).
- De Paolis L, De Ciucis CG, Peletto S, Cappelli K, Mecocci S, Nervo T, Guardone L, Crescio MI, Pietrucci D, Fruscione F, Gabbianelli F, Turco S, Varello K, Donato GG, Maurella C, Modesto P, Maniaci MG, Chillemi G, Ghelardi A, Razzuoli E. Equus caballus Papillomavirus Type-9 (EcPV9): First Detection in Asymptomatic Italian Horses. Viruses 2022 Sep 15;14(9).
- Li Y, Huang H, Lan T, Wang W, Zhang J, Zheng M, Cao L, Sun W, Lu H. First detection and complete genome analysis of the Lyon IARC polyomavirus in China from samples of diarrheic cats. Virus Genes 2021 Jun;57(3):284-288.
- Munday JS, Hardcastle MR, Sim M. Detection of a Putative Novel Papillomavirus Type within a Large Exophytic Papilloma on the Fetlock of a Horse. Pathogens 2020 Oct 5;9(10).
- Hibi H, Hatama S, Obata A, Shibahara T, Kadota K. Laryngeal squamous cell carcinoma and papilloma associated with Equus caballus papillomavirus 2 in a horse. J Vet Med Sci 2019 Jul 19;81(7):1029-1033.
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