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Home / Equine Research Bank / Vet Immunol Immunopathol / Kinetics of plasma procalcitonin, soluble CD14, CCL2 and IL-...
Veterinary immunology and immunopathology2016; 184; 29-35; doi: 10.1016/j.vetimm.2016.12.010

Kinetics of plasma procalcitonin, soluble CD14, CCL2 and IL-10 after a sublethal infusion of lipopolysaccharide in horses.

Abstract: Endotoxemia represents a significant clinical and economic problem for the equine industry. This study assesses the kinetics of soluble CD14 (sCD14), chemokine (CC motif) ligand 2 (CCL2), interleukin 10 (IL-10) and plasma procalcitonin (PCT) in healthy horses after the intravenous infusion of lipopolysaccharide (LPS). The aim was to contribute to the basic understanding of the equine species-specific kinetics of these molecules in response to LPS exposure, which could support further findings in clinical studies and identify valuable inflammatory biomarkers for equine practice. Eleven healthy horses were involved in this experimental in vivo study. Horses were classified as healthy before the LPS infusion. After the pre-infusion blood collection (T0), all horses received an infusion of E. coli endotoxin (30ng/kg over 30min). Data and samples were collected 1h (T1), 2 (T2), 3 (T3) and 24h (T24) after infusion. Plasma sCD14, CCL2 and IL-10 were evaluated with a fluorescent bead-based assay, while PCT was evaluated with an equine PCT ELISA assay. A one-way ANOVA test was performed between each blood-sampling time for PCT, sCD14 and IL-10, and a Friedman test was performed for CCL2. Plasma PCT, IL-10 and CCL2 concentrations increased statistically significantly at T1, T2 and T3 compared to T0. No statistically significant differences were found between plasma IL-10 and CCL2 concentrations between T0 vs T24, although plasma PCT values remained high 24h after LPS infusion. Plasma sCD14 concentration showed no statistically significant differences for any of sampling times. Our results demonstrate that LPS injection into healthy horses results in PCT, CCL2 and IL-10 increases in plasma without an increase in sCD14. The increases in PCT, CCL2 and IL-10 are related to the inflammatory response induced by circulating lipopolysaccharide.
Copyright © 2016 Elsevier B.V. All rights reserved.
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Publication Date: 2016-12-28 PubMed ID: 28166929DOI: 10.1016/j.vetimm.2016.12.010Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the effects of a sublethal dose of lipopolysaccharide on the levels of certain molecules, soluble CD14, chemokine ligand 2, interleukin 10 and procalcitonin, in the blood of healthy horses. The study reveals the response of these molecules in horses following lipopolysaccharide infusion.

Research Goal and Methodology

  • This study aimed to understand the reaction of certain molecules, sCD14, CCL2, IL-10, and PCT, in horses when exposed to lipopolysaccharide (LPS), a toxin found in the outer membrane of certain bacteria.
  • The study involved 11 healthy horses, verified as healthy prior the experiment. These horses were intravenously infused with the LPS extracted from E.coli.
  • Blood samples were taken for analysis just before the LPS infusion as a baseline (T0), and then at 1h (T1), 2h (T2), 3h (T3), and 24 hours (T24) following the infusion.
  • The LPS-induced responses of sCD14, CCL2, and IL-10 were examined using a fluorescent bead-based assay, and PCT was evaluated with an equine PCT ELISA assay.
  • The changes in concentrations of these molecules at different points of time after the exposure to LPS were checked via statistical tests – a one-way ANOVA for PCT, sCD14, and IL-10, and a Friedman test for CCL2.

Study Findings

  • The concentrations of PCT, IL-10, and CCL2 in blood plasma were observed to rise significantly at times T1, T2, and T3 as compared to T0.
  • There was no statistically significant difference in the levels of IL-10 and CCL2 at T0 compared to T24, indicating that their concentrations decreased back to baseline levels 24 hours after LPS infusion.
  • In contrast, plasma PCT levels remained elevated 24 hours after LPS infusion.
  • The plasma concentration of sCD14 showed no significant changes at any time point after the LPS infusion.
  • The study concluded that LPS infusion in healthy horses increases plasma concentrations of PCT, CCL2, and IL-10 due to the inflammatory response triggered by the presence of LPS, while it does not affect sCD14 levels.

Cite This Article

APA
Bonelli F, Meucci V, Divers TJ, Wagner B, Intorre L, Sgorbini M. (2016). Kinetics of plasma procalcitonin, soluble CD14, CCL2 and IL-10 after a sublethal infusion of lipopolysaccharide in horses. Vet Immunol Immunopathol, 184, 29-35. https://doi.org/10.1016/j.vetimm.2016.12.010

Publication

Veterinary immunology and immunopathology
ISSN: 1873-2534
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 184
Pages: 29-35

Researcher Affiliations

Bonelli, Francesca
  • The Department of Veterinary Sciences, University of Pisa (Bonelli, Intorre, Meucci, Sgorbini), 56122, San Piero a Grado (PI), Italy. Electronic address: fbonelli@vet.unipi.it.
Meucci, Valentina
  • The Department of Veterinary Sciences, University of Pisa (Bonelli, Intorre, Meucci, Sgorbini), 56122, San Piero a Grado (PI), Italy.
Divers, Thomas J
  • College of Veterinary Medicine, Cornell University (Wagner, Divers), Ithaca, NY 14853, United States.
Wagner, Bettina
  • College of Veterinary Medicine, Cornell University (Wagner, Divers), Ithaca, NY 14853, United States.
Intorre, Luigi
  • The Department of Veterinary Sciences, University of Pisa (Bonelli, Intorre, Meucci, Sgorbini), 56122, San Piero a Grado (PI), Italy.
Sgorbini, Micaela
  • The Department of Veterinary Sciences, University of Pisa (Bonelli, Intorre, Meucci, Sgorbini), 56122, San Piero a Grado (PI), Italy.

MeSH Terms

  • Animals
  • Calcitonin / blood
  • Calcitonin / physiology
  • Chemokine CCL2 / blood
  • Chemokine CCL2 / physiology
  • Female
  • Horse Diseases / blood
  • Horse Diseases / immunology
  • Horse Diseases / physiopathology
  • Horses / blood
  • Horses / immunology
  • Inflammation / blood
  • Inflammation / immunology
  • Inflammation / physiopathology
  • Inflammation / veterinary
  • Interleukin-10 / blood
  • Interleukin-10 / physiology
  • Lipopolysaccharide Receptors / blood
  • Lipopolysaccharide Receptors / physiology
  • Lipopolysaccharides / pharmacology

Citations

This article has been cited 12 times.
  1. Mukhopadhyay A, Cook SR, SanMiguel P, Ekenstedt KJ, Taylor SD. TLR4 and MD2 variation among horses with differential TNFα baseline concentrations and response to intravenous lipopolysaccharide infusion. Sci Rep 2023 Jan 27;13(1):1486.
    doi: 10.1038/s41598-023-27956-ypubmed: 36707633google scholar: lookup
  2. Taylor SD, Serpa PBS, Santos AP, Hart KA, Vaughn SA, Moore GE, Mukhopadhyay A, Page AE. Effects of intravenous administration of peripheral blood-derived mesenchymal stromal cells after infusion of lipopolysaccharide in horses. J Vet Intern Med 2022 Jul;36(4):1491-1501.
    doi: 10.1111/jvim.16447pubmed: 35698909google scholar: lookup
  3. López-Martínez MJ, Escribano D, Martínez-Miró S, Ramis G, Manzanilla EG, Tecles F, Martínez-Subiela S, Cerón JJ. Measurement of procalcitonin in saliva of pigs: a pilot study. BMC Vet Res 2022 Apr 15;18(1):139.
    doi: 10.1186/s12917-022-03240-5pubmed: 35428339google scholar: lookup
  4. Meucci V, Orsetti C, Sgorbini M, Battaglia F, Cresci M, Bonelli F. Can Procalcitonin Be Dosed in Bovine Milk Using a Commercial ELISA Kit?. Animals (Basel) 2022 Jan 25;12(3).
    doi: 10.3390/ani12030289pubmed: 35158613google scholar: lookup
  5. Nocera I, Bonelli F, Vitale V, Meucci V, Conte G, Jose-Cunilleras E, Gracia-Calvo LA, Sgorbini M. Evaluation of Plasmatic Procalcitonin in Healthy, and in Systemic Inflammatory Response Syndrome (SIRS) Negative or Positive Colic Horses. Animals (Basel) 2021 Jul 6;11(7).
    doi: 10.3390/ani11072015pubmed: 34359143google scholar: lookup
  6. Hagen A, Lehmann H, Aurich S, Bauer N, Melzer M, Moellerberndt J, Patané V, Schnabel CL, Burk J. Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture. Front Bioeng Biotechnol 2020;8:613621.
    doi: 10.3389/fbioe.2020.613621pubmed: 33553119google scholar: lookup
  7. Perez-Ecija A, Buzon-Cuevas A, Aguilera-Aguilera R, Gonzalez-De Cara C, Mendoza Garcia FJ. Reference intervals of acute phase proteins in healthy Andalusian donkeys and response to experimentally induced endotoxemia. J Vet Intern Med 2021 Jan;35(1):580-589.
    doi: 10.1111/jvim.16015pubmed: 33336874google scholar: lookup
  8. Scavone D, Sgorbini M, Borges AS, Oliveira-Filho JP, Vitale V, Paltrinieri S. Serial measurements of Paraoxonase-1 (PON-1) activity in horses with experimentally induced endotoxemia. BMC Vet Res 2020 Nov 4;16(1):422.
    doi: 10.1186/s12917-020-02629-4pubmed: 33148245google scholar: lookup
  9. Anderson MJ, Ibrahim AS, Cooper BR, Woolcock AD, Moore GE, Taylor SD. Effects of administration of ascorbic acid and low-dose hydrocortisone after infusion of sublethal doses of lipopolysaccharide to horses. J Vet Intern Med 2020 Nov;34(6):2710-2718.
    doi: 10.1111/jvim.15896pubmed: 33026127google scholar: lookup
  10. Battaglia F, Meucci V, Tognetti R, Bonelli F, Sgorbini M, Lubas G, Pretti C, Intorre L. Procalcitonin Detection in Veterinary Species: Investigation of Commercial ELISA Kits. Animals (Basel) 2020 Aug 26;10(9).
    doi: 10.3390/ani10091511pubmed: 32859059google scholar: lookup
  11. Sala G, Orsetti C, Meucci V, De Marchi L, Sgorbini M, Bonelli F. Case-Control Study: Endogenous Procalcitonin and Protein Carbonylated Content as a Potential Biomarker of Subclinical Mastitis in Dairy Cows. Vet Sci 2023 Nov 24;10(12).
    doi: 10.3390/vetsci10120670pubmed: 38133221google scholar: lookup
  12. Nocera I, Sgorbini M, Meucci V, Gracia-Calvo LA, Tapio H, Camisi M, Sala G, Citi S. Procalcitonin and carbonylated protein concentrations in equine synovial fluid. Vet Res Commun 2024 Apr;48(2):1263-1269.
    doi: 10.1007/s11259-023-10280-1pubmed: 38127161google scholar: lookup
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