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Theriogenology2020; 152; 1-7; doi: 10.1016/j.theriogenology.2020.04.006

Kisspeptin/GnRH1 system in Leydig cells of horse (Equus caballus): Presence and function.

Abstract: The objectives of this study were to evaluate in horse testes the expression of kisspeptin (KiSS) and GnRH1 neuropeptides and their cognate receptors, KiSS1R and GnRH1R, as well as their action on testosterone, GnRH1, prostaglandin F2α (PGF2α), and PGE2 synthesis and cyclooxygenase 1 (COX1) and COX2 activity by Leydig cells in vitro. Testes were obtained from 9 sexually mature horses by surgical castration. Immunohistochemistry, evidenced the presence of KiSS, KiSS1R, GnRH, and GnRH1R in Leydig cells, whereas germinal and Sertoli cells were positive only for GnRH1. Transcripts for both neuropeptides and their cognate receptors were revealed in isolated Leydig cells by RT-PCR. Isolated and purified Leydig cells were in vitro cultured with agonists and antagonists of KiSS (KiSS-10 and KiSS-234, respectively) and GnRH1 (buserelin and antide, respectively). KiSS-10 and buserelin increased (P < 0.01) COX1 activity and testosterone and PGF2α basal secretion, while decreased (P < 0.01) that of PGE2. KiSS-10 and buserelin did not affect COX2 activity. GnRH1 basal production was increased (P < 0.01) by KiSS-10, but not by buserelin. Antide counteracted the KiSS and GnRH1 effects, whereas KiSS-234 influence only those of KiSS. Summarizing, the KiSS/GnRH1 system is present in horse Leydig cells and modulates their endocrine activity. In particular, the endocrine effects of KiSS are mediated by GnRH1, so suggesting that hypothalamic-like interaction between KiSS and GnRH1 occurs also in Leydig cells.
Copyright © 2020. Published by Elsevier Inc.
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Publication Date: 2020-04-15 PubMed ID: 32339963DOI: 10.1016/j.theriogenology.2020.04.006Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the presence and function of the kisspeptin/GnRH1 system in the Leydig cells of a horse’s testes, and how they influence the synthesis of testosterone, GnRH1, prostaglandin F2α, and PGE2, as well as cyclooxygenase 1 and cyclooxygenase 2 activity.

Study objective and Methodology

  • The study aimed to analyze the expression of kisspeptin (KiSS) and GnRH1 neuropeptides and their receptors (KiSS1R and GnRH1R) in horse testes. Additionally, it sought to understand their role in producing testosterone, GnRH1, prostaglandin F2α (PGF2α), and PGE2, and their influence on cyclooxygenase 1 (COX1) and COX2 activity by Leydig cells in vitro.
  • The researchers used testes obtained from 9 sexually mature horses that had undergone surgical castration.
  • The presence of KiSS, KiSS1R, GnRH, and GnRH1R in Leydig cells was confirmed through immunohistochemistry. The germinal and Sertoli cells only tested positive for GnRH1.
  • The presence of the neuropeptides and their receptors were also confirmed in isolated Leydig cells using RT-PCR.

Experiments and Findings

  • The isolated Leydig cells were cultured in vitro with agonists and antagonists of KiSS (KiSS-10 and KiSS-234, respectively) and GnRH1 (buserelin and antide, respectively).
  • Results showed an increase in COX1 activity and the basal secretion of testosterone and PGF2α when the cells were treated with KiSS-10 and buserelin. Alternatively, these same treatments led to decreased PGE2 secretion.
  • It was noted that KiSS-10 and buserelin did not affect COX2 activity.
  • KiSS-10 led to an increased basal production of GnRH1, unlike buserelin.
  • The effects of KiSS and GnRH1 were counteracted by antide, while only the effects of KiSS were influenced by KiSS-234.

Conclusions

  • The research established the presence and function of the KiSS/GnRH1 system in horse Leydig cells. These systems play a critical role in modulating the endocrine activity of these cells.
  • The study suggests that the endocrine effects of KiSS are mediated by GnRH1, indicating a hypothalamic-like interaction between KiSS and GnRH1 within Leydig cells.

Cite This Article

APA
Petrucci L, Maranesi M, Verini Supplizi A, Dall'Aglio C, Mandara MT, Quassinti L, Bramucci M, Miano A, Gobbetti A, Catone G, Boiti C, Zerani M. (2020). Kisspeptin/GnRH1 system in Leydig cells of horse (Equus caballus): Presence and function. Theriogenology, 152, 1-7. https://doi.org/10.1016/j.theriogenology.2020.04.006

Publication

Theriogenology
ISSN: 1879-3231
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 152
Pages: 1-7
PII: S0093-691X(20)30225-9

Researcher Affiliations

Petrucci, Linda
  • Dipartimento di Medicina Veterinaria, Università di Perugia, 06126, Perugia, PG, Italy; Scuola di Bioscienze e Medicina Veterinaria, Università di Camerino, 62032, Camerino, MC, Italy.
Maranesi, Margherita
  • Dipartimento di Medicina Veterinaria, Università di Perugia, 06126, Perugia, PG, Italy. Electronic address: massimo.zerani@unipg.it.
Verini Supplizi, Andrea
  • Dipartimento di Medicina Veterinaria, Università di Perugia, 06126, Perugia, PG, Italy.
Dall'Aglio, Cecilia
  • Dipartimento di Medicina Veterinaria, Università di Perugia, 06126, Perugia, PG, Italy.
Mandara, Maria Teresa
  • Dipartimento di Medicina Veterinaria, Università di Perugia, 06126, Perugia, PG, Italy.
Quassinti, Luana
  • Scuola di Scienze del Farmaco e dei Prodotti della Salute, Università di Camerino, 62032, Camerino, MC, Italy.
Bramucci, Massimo
  • Scuola di Scienze del Farmaco e dei Prodotti della Salute, Università di Camerino, 62032, Camerino, MC, Italy.
Miano, Antonino
  • Scuola di Bioscienze e Medicina Veterinaria, Università di Camerino, 62032, Camerino, MC, Italy.
Gobbetti, Anna
  • Scuola di Bioscienze e Medicina Veterinaria, Università di Camerino, 62032, Camerino, MC, Italy.
Catone, Giuseppe
  • Scuola di Bioscienze e Medicina Veterinaria, Università di Camerino, 62032, Camerino, MC, Italy; Dipartimento di Scienze Veterinarie, Università di Messina, 98122, Messina, ME, Italy.
Boiti, Cristiano
  • Dipartimento di Medicina Veterinaria, Università di Perugia, 06126, Perugia, PG, Italy.
Zerani, Massimo
  • Dipartimento di Medicina Veterinaria, Università di Perugia, 06126, Perugia, PG, Italy; Scuola di Bioscienze e Medicina Veterinaria, Università di Camerino, 62032, Camerino, MC, Italy.

MeSH Terms

  • Animals
  • Cyclooxygenase 1 / genetics
  • Cyclooxygenase 1 / metabolism
  • Cyclooxygenase 2 / genetics
  • Cyclooxygenase 2 / metabolism
  • Gene Expression Regulation
  • Gonadotropin-Releasing Hormone / genetics
  • Gonadotropin-Releasing Hormone / metabolism
  • Horses
  • Kisspeptins / genetics
  • Kisspeptins / metabolism
  • Leydig Cells / metabolism
  • Male
  • Receptors, Kisspeptin-1 / genetics
  • Receptors, Kisspeptin-1 / metabolism
  • Receptors, LHRH / genetics
  • Receptors, LHRH / metabolism

Citations

This article has been cited 7 times.
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    doi: 10.3390/genes13020295pubmed: 35205340google scholar: lookup
  3. Guo HX, Yuan B, Su MT, Zheng Y, Zhang JY, Han DX, Wang HQ, Huang YJ, Jiang H, Zhang JB. Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH. Animals (Basel) 2021 Aug 31;11(9).
    doi: 10.3390/ani11092557pubmed: 34573523google scholar: lookup
  4. Wang H, Liu X, Dong S, Zhou Y, Yu J, Zou M, Ding M, Kang A, Ji N, Zeng X, Zhang X. lncRNA 1700009J07Rik Impaired Male Fertility by Interfering with Sexual Behaviors in Mice. Int J Mol Sci 2025 Jun 17;26(12).
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  5. Marino M, D'Auria R, Mele E, Pastorino GMG, Di Pietro P, D'Angelo S, Della Rocca N, Operto FF, Vecchione C, Fasano S, Pierantoni R, Viggiano A, Meccariello R, Santoro A. The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo. Front Endocrinol (Lausanne) 2023;14:1269334.
    doi: 10.3389/fendo.2023.1269334pubmed: 37900144google scholar: lookup
  6. Santos LC, Dos Anjos Cordeiro JM, da Silva Santana L, Barbosa EM, Santos BR, Mendonça LD, Cunha MCDSG, Machado WM, Santana LR, Kersul MG, Henriques PC, Lopes RA, Snoeck PPDN, Szawka RE, Silva JF. Kisspeptin treatment reverses high prolactin levels and improves gonadal function in hypothyroid male rats. Sci Rep 2023 Oct 5;13(1):16819.
    doi: 10.1038/s41598-023-44056-zpubmed: 37798396google scholar: lookup
  7. Chu X, Javed A, Ashraf MF, Gao X, Jiang S. Primary culture and endocrine functional analysis of Leydig cells in ducks (Anas platyrhynchos). Front Endocrinol (Lausanne) 2023;14:1195618.
    doi: 10.3389/fendo.2023.1195618pubmed: 37347106google scholar: lookup
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