Lactobacillus hayakitensis, L. equigenerosi and L. equi, predominant lactobacilli in the intestinal flora of healthy thoroughbreds.
Abstract: To detect the predominant lactobacilli in the intestinal flora of healthy thoroughbreds, we isolated lactobacilli from the feces of nine thoroughbreds (five males and four females; 0-15-year-old). The isolated lactobacilli comprise 17 species (37 strains), and they were classified into five groups: Lactobacillus salivarius (6 species), L. reuteri (6 species), Lactobacillus delbrueckii (3 species), L. buchneri (1 species) and L. vitulinus (1 species). On the basis of 16S rRNA gene sequences, we identified 3 other phylogenetic relatives belonging to the genus Lactobacillus. These results suggest that the intestinal flora of thoroughbreds may comprise many species of the genus Lactobacillus. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analyses of the 340-bp fragments of the 16S rRNA genes from the same nine fecal samples showed that L. hayakitensis, L. equigenerosi and L. equi are contained in all the samples, suggesting that these species are predominant lactobacilli in the intestinal flora of thoroughbreds.
Publication Date: 2010-02-19 PubMed ID: 20163646DOI: 10.1111/j.1740-0929.2009.00633.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates the types of natural bacteria, specifically lactobacilli, found in the gut of healthy thoroughbred horses. The study reveals three specific lactobacillus species – L. hayakitensis, L. equigenerosi and L. equi – as the predominant forms of these bacteria.
Methods and Findings
In the study, the researchers:
- Collected fecal samples from nine healthy thoroughbreds – five males and four females aged between zero to fifteen years.
- Isolated lactobacilli from these samples. Lactobacilli belong to a group of bacteria that play a crucial role in the gut health of various organisms.
- Identified seventeen different species of lactobacilli, which were classified into five groups: L. salivarius, L. reuteri, L. delbrueckii, L. buchneri, and L. vitulinus.
- Discovered, based on 16S rRNA gene sequences, three other phylogenetic relatives that fall within the genus Lactobacillus. 16S rRNA gene sequencing is a technique used for bacterial identification and taxonomy.
Significance of the Results
The study’s findings are significant because:
- They demonstrate that the intestinal flora of thoroughbred horses comprises a variety of Lactobacillus species, contributing to the horses’ digestive health.
- Most importantly, the researchers identified three specific lactobacillus species – L. hayakitensis, L. equigenerosi and L. equi – in all the analyzed samples. These species were present in all the fecal samples analyzed using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), a molecular fingerprinting technique for separating and identifying microorganisms.
- This discovery suggests that those three species are the key lactobacilli in the gut of thoroughbred horses, thus providing a better understanding of the microbial ecology within the equine gut. This information could be useful for further research into equine health and disease, as well as the development of probiotics specifically tailored for horses.
Cite This Article
APA
Morita H, Nakano A, Shimazu M, Toh H, Nakajima F, Nagayama M, Hisamatsu S, Kato Y, Takagi M, Takami H, Akita H, Matsumoto M, Masaoka T, Murakami M.
(2010).
Lactobacillus hayakitensis, L. equigenerosi and L. equi, predominant lactobacilli in the intestinal flora of healthy thoroughbreds.
Anim Sci J, 80(3), 339-346.
https://doi.org/10.1111/j.1740-0929.2009.00633.x Publication
Researcher Affiliations
- School of Veterinary Medicine, Azabu University, Sagamihara, Japan. morita@azabu-u.ac.jp
MeSH Terms
- Animals
- Female
- Horses / microbiology
- Intestines / microbiology
- Lactobacillus / isolation & purification
- Male
Citations
This article has been cited 15 times.- Li XB, Huang XX, Li Q, Li XY, Li JH, Li C, He LJ, Jing HX, Yang KL. Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.. BMC Vet Res 2022 Nov 17;18(1):407.
- Gilroy R, Leng J, Ravi A, Adriaenssens EM, Oren A, Baker D, La Ragione RM, Proudman C, Pallen MJ. Metagenomic investigation of the equine faecal microbiome reveals extensive taxonomic diversity.. PeerJ 2022;10:e13084.
- Li Y, Liu C, Liu Q, Liu W. Comparative Genomic Analysis Reveals Intestinal Habitat Adaptation of Ligilactobacillus equi Rich in Prophage and Degrading Cellulase.. Molecules 2022 Mar 14;27(6).
- Meinders RI, Mendoza M, Dickey AN, Scholl EH, Hassan HM. Complete Genome Sequences of Six Lactobacilli Isolated from American Quarter Horses.. Microbiol Resour Announc 2020 Nov 19;9(47).
- Takahara M, Takaki A, Hiraoka S, Adachi T, Shimomura Y, Matsushita H, Nguyen TTT, Koike K, Ikeda A, Takashima S, Yamasaki Y, Inokuchi T, Kinugasa H, Sugihara Y, Harada K, Eikawa S, Morita H, Udono H, Okada H. Berberine improved experimental chronic colitis by regulating interferon-γ- and IL-17A-producing lamina propria CD4(+) T cells through AMPK activation.. Sci Rep 2019 Aug 15;9(1):11934.
- Mythen SM, Devendran S, Méndez-García C, Cann I, Ridlon JM. Targeted Synthesis and Characterization of a Gene Cluster Encoding NAD(P)H-Dependent 3α-, 3β-, and 12α-Hydroxysteroid Dehydrogenases from Eggerthella CAG:298, a Gut Metagenomic Sequence.. Appl Environ Microbiol 2018 Apr 1;84(7).
- Toh H, Nakano A, Nguyen CT, Mimura I, Arakawa K, Tashiro K, Kikusui T, Morita H. Draft Genome Sequence of Coccoid Lactobacillus equigenerosi NRIC 0697T Isolated from the Gastrointestinal Tracts of Healthy Thoroughbreds.. Genome Announc 2016 Feb 4;4(1).
- Takagi M, Nakano A, Toh H, Oshima K, Arakawa K, Nakajima F, Tashiro K, Kikusui T, Yanagida F, Morita H. Draft Genome Sequence of Streptococcus orisasini SH06, Isolated from a Healthy Thoroughbred Gastrointestinal Tract.. Genome Announc 2016 Jan 14;4(1).
- Tanabe S, Suzuki T, Wasano Y, Nakajima F, Kawasaki H, Tsuda T, Nagamine N, Tsurumachi T, Sugaya K, Akita H, Takagi M, Takagi K, Inoue Y, Asai Y, Morita H. Anti-inflammatory and Intestinal Barrier-protective Activities of Commensal Lactobacilli and Bifidobacteria in Thoroughbreds: Role of Probiotics in Diarrhea Prevention in Neonatal Thoroughbreds.. J Equine Sci 2014;25(2):37-43.
- O'Donnell MM, Harris HM, O'Toole PW, Ross RP. The Genome of the Predominant Equine Lactobacillus Species, Lactobacillus equi, Is Reflective of Its Lifestyle Adaptations to an Herbivorous Host.. Genome Announc 2014 Jan 16;2(1).
- Steelman SM, Chowdhary BP, Dowd S, Suchodolski J, Janečka JE. Pyrosequencing of 16S rRNA genes in fecal samples reveals high diversity of hindgut microflora in horses and potential links to chronic laminitis.. BMC Vet Res 2012 Nov 27;8:231.
- St-Pierre B, de la Fuente G, O'Neill S, Wright AD, Al Jassim R. Analysis of stomach bacterial communities in Australian feral horses.. Mol Biol Rep 2013 Jan;40(1):369-76.
- Botha M, Botes M, Loos B, Smith C, Dicks LM. Lactobacillus equigenerosi strain Le1 invades equine epithelial cells.. Appl Environ Microbiol 2012 Jun;78(12):4248-55.
- Perkins GA, den Bakker HC, Burton AJ, Erb HN, McDonough SP, McDonough PL, Parker J, Rosenthal RL, Wiedmann M, Dowd SE, Simpson KW. Equine stomachs harbor an abundant and diverse mucosal microbiota.. Appl Environ Microbiol 2012 Apr;78(8):2522-32.
- O'Donnell MM, Forde BM, Neville B, Ross PR, O'Toole PW. Carbohydrate catabolic flexibility in the mammalian intestinal commensal Lactobacillus ruminis revealed by fermentation studies aligned to genome annotations.. Microb Cell Fact 2011 Aug 30;10 Suppl 1(Suppl 1):S12.
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