Limited similarity between plasmids encoding CTX-M-1 β-lactamase in Escherichia coli from humans, pigs, cattle, organic poultry layers and horses in Denmark.
Abstract: CTX-M-1 is a common extended-spectrum β-lactamase (ESBL) in Escherichia coli from animals and is often detected among human clinical isolates. The objective of this study was to investigate the epidemiological relationship between CTX-M-1-producing E. coli isolated from patients and animals in Denmark between 2006 and 2010. In total, 65 CTX-M-1-producing isolates from patients (n=22), pigs (n=21), cattle (n=4), organic poultry layers (n=3) and horses (n=15) were typed by pulsed-field gel electrophoresis (PFGE). Plasmids harbouring bla were characterised by S1 PFGE, PCR-based replicon typing, plasmid multilocus sequence typing, restriction fragment length polymorphism, and sequencing. Human and animal strains were unrelated based on PFGE. IncI1 was more common in human isolates (13/22) than in animal isolates (7/43), whereas the opposite trend was observed for IncN (5/22 human isolates and 24/43 animal isolates). Full characterisation of the plasmids harbouring bla revealed host-specific patterns in the distribution of plasmid types, with specific IncI1, IncN and IncH1 plasmid subtypes being predominant in humans, livestock and horses, respectively. Three indistinguishable human, bovine and porcine IncI1/ST49 plasmids had high nucleotide sequence homology and differed by the presence of IS66 elements in the bovine plasmid and the absence of one gene within the microcin-encoding operon in the human plasmid. In conclusion, this work suggests a minor contribution by animals to the occurrence of CTX-M-1 in human E. coli infections in Denmark during the study period.
Copyright © 2015 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.
Publication Date: 2015-05-07 PubMed ID: 27873662DOI: 10.1016/j.jgar.2015.03.009Google Scholar: Lookup
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Summary
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The research investigates the epidemiological relationship between CTX-M-1-producing E. coli found in humans and animals in Denmark from 2006 to 2010. The researchers concluded that CTX-M-1 in human E. coli infections in Denmark during the study period might be minimally influenced by animals.
Research Methodology
- The study analysed 65 CTX-M-1-producing isolates derived from various sources – patients (22), pigs (21), cattle (4), organic poultry layers (3) and horses (15).
- The researchers employed pulsed-field gel electrophoresis (PFGE) to type these isolates.
- Further characterisation of the plasmids harbouring the bla gene (a gene implicated in antibiotic resistance) was accomplished through various methods such as S1 PFGE, PCR-based replicon typing, plasmid multilocus sequence typing, restriction fragment length polymorphism, and sequencing.
Findings
- The researchers found that the human and animal strains of E. Coli were unrelated based on PFGE.
- They observed a higher frequency of IncI1 in human isolates (13 out of 22) than in animal isolates (7 out of 43), whereas IncN was found to be more common among animal isolates (24 out of 43 versus 5 out of 22 in humans).
- The full characterisation of the plasmids containing the bla gene revealed host-specific patterns in their distribution. For instance, specific IncI1, IncN and IncH1 plasmid subtypes were predominantly found in humans, livestock and horses, respectively.
- One notable discovery was three identical human, bovine and porcine IncI1/ST49 plasmids. They all had high nucleotide sequence homology but differed by the presence of IS66 elements in the bovine plasmid and the absence of one gene within the microcin-encoding operon in the human plasmid.
Conclusions
- The research infers a minor contribution by animals to the occurrence of CTX-M-1 in human E. coli infections in Denmark during the study period. This suggests that the E. coli strains in humans aren’t largely derived from animal sources but could be resulting from other causes that need further investigation.
Cite This Article
APA
Jakobsen L, Bortolaia V, Bielak E, Moodley A, Olsen SS, Hansen DS, Frimodt-Møller N, Guardabassi L, Hasman H.
(2015).
Limited similarity between plasmids encoding CTX-M-1 β-lactamase in Escherichia coli from humans, pigs, cattle, organic poultry layers and horses in Denmark.
J Glob Antimicrob Resist, 3(2), 132-136.
https://doi.org/10.1016/j.jgar.2015.03.009 Publication
Researcher Affiliations
- Microbiology and Infection Control, Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S, Denmark. Electronic address: lottejakobsen@gmail.com.
- Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark.
- National Food Institute, Technical University of Denmark, 204 Kemitorvet, DK-2800 Kgs., Lyngby, Denmark.
- Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark.
- Microbiology and Infection Control, Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S, Denmark.
- Department of Clinical Microbiology, Copenhagen University Hospital Herlev, 75 Herlev Ringvej, DK-2730 Herlev, Denmark.
- Department of Clinical Microbiology, Copenhagen University Hospital Rigshospitalet, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark.
- Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 4, DK-1870 Frederiksberg C, Denmark.
- National Food Institute, Technical University of Denmark, 204 Kemitorvet, DK-2800 Kgs., Lyngby, Denmark.
Citations
This article has been cited 14 times.- Anderson REV, Chalmers G, Murray R, Mataseje L, Pearl DL, Mulvey M, Topp E, Boerlin P. Characterization of Escherichia coli and Other Enterobacterales Resistant to Extended-Spectrum Cephalosporins Isolated from Dairy Manure in Ontario, Canada.. Appl Environ Microbiol 2023 Feb 28;89(2):e0186922.
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