Lipid composition and cholesterol esterification in serum lipoprotein fraction of the horse, Equus caballus.
Abstract: 1. Changes in lipid components of lipoproteins during incubation of horse serum at 37 degrees C were investigated. In non-incubated serum, cholesterol and lecithin existed predominantly in alpha-lipoprotein or in high-density lipoprotein (HDL). Lysolecithin was mainly associated with the fraction with density above 1.21. 2. When serum was separated into alpha- and beta-lipoproteins by the heparin precipitation method after 1 hr incubation, the decrease in alpha-lipoprotein free cholesterol and lecithin was about four times that in beta-lipoprotein counterparts. 3. When serum lipoproteins were separated by ultracentrifugation, the decrease in each lipoprotein free cholesterol was closely paralleled with that in lecithin. 4. HDL appeared to be a preferential substrate for the lecithin: cholesterol acyltransferase reaction. 5. Disc electrophoretic patterns indicated significant differences in the composition of horse serum lipoproteins from those of human and rat.
Publication Date: 1979-01-01 PubMed ID: 318421DOI: 10.1016/0305-0491(79)90275-xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research investigates changes in lipid components of lipoproteins in horse serum, focusing on how these changes occur under different conditions and comparing the composition of horse serum lipoproteins to those of humans and rats.
Methodology and Key Findings
- The research aimed to explore changes in lipid components of lipoproteins during incubation of horse serum at 37 degrees Celsius. The researchers observed that in non-incubated serum, cholesterol and lecithin existed predominantly in alpha-lipoprotein or in high-density lipoprotein (HDL).
- The study further observed that lysolecithin, a fatty acid and a component of lecithin, was primarily associated with the fraction with density above 1.21.
- The researchers also carried out an experiment where the serum was separated into alpha- and beta-lipoproteins by the heparin precipitation method after one hour of incubation. The results showed that the decrease in alpha-lipoprotein free cholesterol and lecithin was about four times that in beta-lipoprotein counterparts.
- In another part of the study, when serum lipoproteins were separated by ultracentrifugation, the decrease in each lipoprotein’s free cholesterol was closely paralleled with that in lecithin. This suggests a close relationship between the presence of these two components in the lipoproteins.
- The study also highlighted that the HDL appeared to be a preferential substrate for the lecithin: cholesterol acyltransferase reaction. This enzyme reaction essentially involves the conversion of free cholesterol into cholesteryl ester. This suggests that the HDL is an optimal environment or substrate for this biochemical process.
Comparative Analysis
- Lastly, the researchers made a comparative analysis of the horse serum lipoproteins with those of humans and rats. The disc electrophoretic patterns, a method to analyze the composition and characteristics of proteins, revealed significant differences in the composition of horse serum lipoproteins from those found in humans and rats.
Cite This Article
APA
Yamamoto M, Tanaka Y, Sugano M.
(1979).
Lipid composition and cholesterol esterification in serum lipoprotein fraction of the horse, Equus caballus.
Comp Biochem Physiol B, 63(3), 441-449.
https://doi.org/10.1016/0305-0491(79)90275-x Publication
Researcher Affiliations
- Department of Chemistry, Kurume University School of Medicine, Japan.
MeSH Terms
- Animals
- Cholesterol Esters / blood
- Female
- Horses / blood
- Lipids / blood
- Lipoproteins / blood
- Male
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