Loading-induced changes in synovial fluid affect cartilage metabolism.
Abstract: The purpose of this study was to determine whether changes in the synovial fluid (SF) induced by in vivo loading can induce an alteration in the metabolic activity of chondrocytes in vitro. Therefore, SF was collected from ponies after a period of box rest and after they had exercise for a week. Normal, unloaded articular cartilage explants were cultured in 20% solutions of these SFs for 4 days and chondrocyte activity was determined by glycosaminoglycan (GAG) turnover. In explants cultured in post-exercise SF, GAG synthesis was enhanced and GAG release was diminished when compared to cultures in pre-exercise SF. SF analysis showed that levels of insulin-like growth factors (IGF-I and IGF-II) tended to be higher in post-exercise SF, while no differences were found in metalloproteinase activity, hyaluronic acid and protein concentrations. This study showed that anabolic effects of joint loading on cartilage are, at least partially, mediated by alterations in the SF.
Publication Date: 1998-07-17 PubMed ID: 9667623DOI: 10.1093/rheumatology/37.6.671Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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This study investigates how changes in the fluid located in the joints (synovial fluid) due to physical exertion can affect the activities of cartilage cells (chondrocytes). The research found that these changes could stimulate synovial fluid’s anabolic effects on cartilage, suggesting a connection between physical exercise, joint health, and cartilage metabolism.
Objective of the Study
- The primary objective of this study was to explore how in vivo loading-induced changes in synovial fluid might impact the metabolic activity of chondrocytes. This was studied using ponies at rest and post-exercise.
Methodology and Experiment Design
- Synovial fluid was harvested from ponies following a resting phase as well as post-exercise.
- The experiment then involved culturing normal, unloaded articular cartilage explants in 20% solutions of these harvested synovial fluids for four days.
- The researchers then evaluated chondrocyte activity through assessing glycosaminoglycan (GAG) turnover.
Findings on Chondrocyte Activity
- In comparison to pre-exercise synovial fluid conditions, it was found that post-exercise synovial fluid conditions promoted an increase in GAG synthesis and reduced GAG release.
Synovial Fluid Analysis
- Synovial fluid analysis post-exercise demonstrated an upward trend in insulin-like growth factor levels (IGF-I and IGF-II).
- No significant differences were identified in terms of metalloproteinase activity, hyaluronic acid, and protein concentrations in post-exercise synovial fluid compared to pre-exercise synovial fluid.
Research Conclusion
- The study concluded that joint loading’s anabolic effects on cartilage could be partially driven by alterations in the synovial fluid due to in vivo loading or physical exercise.
Cite This Article
APA
Van den Hoogen BM, van de Lest CH, van Weeren PR, Lafeber FP, Lopes-Cardozo M, van Golde LM, Barneveld A.
(1998).
Loading-induced changes in synovial fluid affect cartilage metabolism.
Br J Rheumatol, 37(6), 671-676.
https://doi.org/10.1093/rheumatology/37.6.671 Publication
Researcher Affiliations
- Department of General and Large Animal Surgery, Utrecht University, The Netherlands.
MeSH Terms
- Animals
- Carpus, Animal
- Cartilage, Articular / cytology
- Cartilage, Articular / metabolism
- Horses / physiology
- Hyaluronic Acid / metabolism
- Insulin-Like Growth Factor I / metabolism
- Insulin-Like Growth Factor II / metabolism
- Male
- Metalloendopeptidases / metabolism
- Organ Culture Techniques
- Physical Conditioning, Animal
- Proteins / metabolism
- Synovial Fluid / metabolism
- Weight-Bearing / physiology
Citations
This article has been cited 5 times.- Tarantino D, Theysmans T, Mottola R, Verbrugghe J. High-Intensity Training for Knee Osteoarthritis: A Narrative Review.. Sports (Basel) 2023 Apr 20;11(4).
- Messier SP, Mihalko SL, Beavers DP, Nicklas BJ, DeVita P, Carr JJ, Hunter DJ, Lyles M, Guermazi A, Bennell KL, Loeser RF. Effect of High-Intensity Strength Training on Knee Pain and Knee Joint Compressive Forces Among Adults With Knee Osteoarthritis: The START Randomized Clinical Trial.. JAMA 2021 Feb 16;325(7):646-657.
- Carballo CB, Coelho TRP, de Holanda Afonso RC, Faria JCO, Alves T, Monte SM, Ventura Matioszek GM, Moura-Neto V, Brito JM. Osteoarthritic Synovial Fluid and TGF-β1 Induce Interleukin-18 in Articular Chondrocytes.. Cartilage 2020 Jul;11(3):385-394.
- Albro MB, Cigan AD, Nims RJ, Yeroushalmi KJ, Oungoulian SR, Hung CT, Ateshian GA. Shearing of synovial fluid activates latent TGF-β.. Osteoarthritis Cartilage 2012 Nov;20(11):1374-82.
- Brand JA, McAlindon TE, Zeng L. A 3D system for culturing human articular chondrocytes in synovial fluid.. J Vis Exp 2012 Jan 31;(59):e3587.
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