Local and systemic antibody production in horses affected with chronic obstructive pulmonary disease.
Abstract: An enzyme-linked immunosorbent assay (ELISA) was used to quantify isotype-specific antibody to Micropolyspora faeni and to Aspergillus fumigatus in the sera and bronchoalveolar lavage fluid (BALF) of normal horses, horses with chronic obstructive pulmonary disease (COPD) and horses with other chronic respiratory diseases. Elevated antibody levels were not detected in the sera of affected horses. However, both IgE and IgA antibody to both allergens was significantly elevated in BALF in COPD affected horses sampled both when symptomatic and asymptomatic. Elevated levels were also found in animals that developed a chronic cough after an acute onset with symptoms compatible with a respiratory virus infection. In one animal a ten fold increase in IgE antibody to the two allergens developed after an interval of 7 weeks. These findings are supportive of a central role of local IgE antibody to mould allergens in the immunopathogenesis of COPD, and also suggest that respiratory viral infection may predispose to the development of COPD in some horses.
Publication Date: 1993-10-01 PubMed ID: 8291200DOI: 10.1016/0165-2427(93)90081-eGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article is about a study that investigates the role of local antibodies in horses with chronic obstructive pulmonary disease (COPD). It indicates that elevated levels of certain specific antibodies in the bronchoalveolar lavage fluid (BALF), not seen in serum, of these horses potentially implicates these antibodies in the development and course of this disease.
Objective of the Research
- The primary aim of this research was to understand the involvement of specific types of antibodies to the allergens Micropolyspora faeni and Aspergillus fumigatus in horses suffering from COPD and other respiratory diseases.
Methodology
- An enzyme-linked immunosorbent assay (ELISA) was employed to measure isotype-specific antibody levels in sera and BALF of horses categorized into: healthy, suffering from COPD, and ones with other chronic respiratory ailments.
- The variations in antibody levels were then compared across these different groups.
Key Findings
- Higher levels of IgE and IgA antibodies to the allergens were identified in the BALF from horses affected by COPD, both when conditions were symptomatic and asymptomatic. These antibody levels did not show an increase in serum.
- The increase in antibodies was also noted in horses that developed chronic cough following symptoms corresponding to a respiratory viral infection.
- In an observed case, the IgE antibody level to both allergens increased tenfold over a period of seven weeks.
Implications of the Research
- The results lend support to the hypothesis that local IgE antibodies reacting to mold allergens play a crucial role in the pathogenesis of COPD in horses.
- The data also indicate that respiratory viral infections could make horses more susceptible to developing COPD.
In conclusion, this research deepens the understanding of COPD progression in horses and implies that controlling local antibodies could potentially be a therapeutic strategy.
Cite This Article
APA
Halliwell RE, McGorum BC, Irving P, Dixon PM.
(1993).
Local and systemic antibody production in horses affected with chronic obstructive pulmonary disease.
Vet Immunol Immunopathol, 38(3-4), 201-215.
https://doi.org/10.1016/0165-2427(93)90081-e Publication
Researcher Affiliations
- University of Edinburgh, Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, Roslin, UK.
MeSH Terms
- Animals
- Antibodies, Bacterial / biosynthesis
- Antibodies, Fungal / biosynthesis
- Aspergillosis / immunology
- Aspergillosis / veterinary
- Aspergillus fumigatus / immunology
- Bacterial Infections / immunology
- Bacterial Infections / veterinary
- Horse Diseases / immunology
- Horse Diseases / microbiology
- Horses
- Lung Diseases, Obstructive / immunology
- Lung Diseases, Obstructive / microbiology
- Lung Diseases, Obstructive / veterinary
- Micromonosporaceae / immunology
Citations
This article has been cited 21 times.- Wyler M, Sage SE, Marti E, White S, Gerber V. Protein microarray allergen profiling in bronchoalveolar lavage fluid and serum of horses with asthma. J Vet Intern Med 2023 Jan;37(1):328-337.
- Klier J, Fuchs S, Winter G, Gehlen H. Inhalative Nanoparticulate CpG Immunotherapy in Severe Equine Asthma: An Innovative Therapeutic Concept and Potential Animal Model for Human Asthma Treatment. Animals (Basel) 2022 Aug 16;12(16).
- Di Pietro R, Dubuc V, Manguin E, Giroux-Lafond R, Bédard C, Boivin R, Lavoie JP, Vesper SJ, Leclere M. Characterization of fungal exposure and dectin-1 expression in healthy horses and horses with severe asthma. Am J Vet Res 2022 May 8;83(6).
- Lo Feudo CM, Stucchi L, Alberti E, Conturba B, Zucca E, Ferrucci F. Intradermal Testing Results in Horses Affected by Mild-Moderate and Severe Equine Asthma. Animals (Basel) 2021 Jul 13;11(7).
- White SJ, Moore-Colyer M, Marti E, Hannant D, Gerber V, Coüetil L, Richard EA, Alcocer M. Antigen array for serological diagnosis and novel allergen identification in severe equine asthma. Sci Rep 2019 Oct 23;9(1):15170.
- White S, Moore-Colyer M, Marti E, Coüetil L, Hannant D, Richard EA, Alcocer M. Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma. J Vet Intern Med 2019 Sep;33(5):2327-2335.
- Klier J, Geis S, Steuer J, Geh K, Reese S, Fuchs S, Mueller RS, Winter G, Gehlen H. A comparison of nanoparticullate CpG immunotherapy with and without allergens in spontaneously equine asthma-affected horses, an animal model. Immun Inflamm Dis 2018 Mar;6(1):81-96.
- Mir IA, Kumar B, Taku A, Faridi F, Bhat MA, Baba NA, Maqbool T. Bacteriological and Molecular Detection of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus in Equines of Northern India. J Equine Sci 2013;24(3):53-5.
- Sarmiento J, Perez B, Morales N, Henriquez C, Vidal L, Folch H, Galecio JS, Morán G. Apoptotic effects of tamoxifen on leukocytes from horse peripheral blood and bronchoalveolar lavage fluid. Vet Res Commun 2013 Dec;37(4):333-8.
- Moran G, Folch H, Henriquez C, Ortloff A, Barria M. Reaginic antibodies from horses with recurrent airway obstruction produce mast cell stimulation. Vet Res Commun 2012 Dec;36(4):251-8.
- Racine J, Gerber V, Feutz MM, Riley CP, Adamec J, Swinburne JE, Couetil LL. Comparison of genomic and proteomic data in recurrent airway obstruction affected horses using Ingenuity Pathway Analysis®. BMC Vet Res 2011 Aug 15;7:48.
- Moran G, Buechner-Maxwell VA, Folch H, Henriquez C, Galecio JS, Perez B, Carrasco C, Barria M. Increased apoptosis of CD4 and CD8 T lymphocytes in the airways of horses with recurrent airway obstruction. Vet Res Commun 2011 Oct;35(7):447-56.
- Morán G, Folch H, Araya O, Burgos R, Barria M. Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay. Vet Res Commun 2010 Dec;34(8):719-26.
- Morán G, Burgos R, Araya O, Folch H. In vitro bioassay to detect reaginic antibodies from the serum of horses affected with recurrent airway obstruction. Vet Res Commun 2010 Jan;34(1):91-9.
- McAleese SM, Miller HR. Cloning and sequencing of the equine and ovine high-affinity IgE receptor beta-and gamma-chain cDNA. Immunogenetics 2003 May;55(2):122-5.
- Venugopalan CS, Beadle RE, Seahorn TL, Holmes EP. Responses of guinea-pig lung parenchymal strips to tracheobronchial lavage fluid from horses affected with summer pasture-associated obstructive pulmonary disease. Vet Res Commun 1998 Nov;22(7):493-503.
- Jentsch MC, Keilhaue A, Wagner B, Rhyner C, Lübke S, Karagulyan M, Arnold C, Lohmann KL, Schnabel CL. Aspergillus fumigatus binding IgA and IgG1 are increased in bronchoalveolar lavage fluid of horses with neutrophilic asthma. Front Immunol 2024;15:1406794.
- Jentsch MC, Lübke S, Schrödl W, Volke D, Krizsan A, Hoffmann R, Kaiser-Thom S, Gerber V, Marti E, Wagner B, Schnabel CL. Immunoproteomics enable broad identification of new Aspergillus fumigatus antigens in severe equine asthma. Front Immunol 2024;15:1347164.
- Dai F, Dalla Costa E, Minero M, Briant C. Does housing system affect horse welfare? The AWIN welfare assessment protocol applied to horses kept in an outdoor group-housing system: The 'parcours'. Anim Welf 2023;32:e22.
- Schnabel CL, Jentsch MC, Lübke S, Kaiser-Thom S, Gerber V, Vrtala S, Huang HJ, Rhyner C, Wagner B, Hoffmann R, Volke D. Immunoproteomics reveal increased serum IgG3/5 binding to Dermatophagoides and yeast protein antigens in severe equine asthma in a preliminary study. Front Immunol 2023;14:1293684.
- Rivolta AA, Bujold AR, Wilmarth PA, Phinney BS, Navelski JP, Horohov DW, Sanz MG. Comparison of the broncoalveolar lavage fluid proteomics between foals and adult horses. PLoS One 2023;18(9):e0290778.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
