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Veterinary immunology and immunopathology1993; 38(3-4); 201-215; doi: 10.1016/0165-2427(93)90081-e

Local and systemic antibody production in horses affected with chronic obstructive pulmonary disease.

Abstract: An enzyme-linked immunosorbent assay (ELISA) was used to quantify isotype-specific antibody to Micropolyspora faeni and to Aspergillus fumigatus in the sera and bronchoalveolar lavage fluid (BALF) of normal horses, horses with chronic obstructive pulmonary disease (COPD) and horses with other chronic respiratory diseases. Elevated antibody levels were not detected in the sera of affected horses. However, both IgE and IgA antibody to both allergens was significantly elevated in BALF in COPD affected horses sampled both when symptomatic and asymptomatic. Elevated levels were also found in animals that developed a chronic cough after an acute onset with symptoms compatible with a respiratory virus infection. In one animal a ten fold increase in IgE antibody to the two allergens developed after an interval of 7 weeks. These findings are supportive of a central role of local IgE antibody to mould allergens in the immunopathogenesis of COPD, and also suggest that respiratory viral infection may predispose to the development of COPD in some horses.
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Publication Date: 1993-10-01 PubMed ID: 8291200DOI: 10.1016/0165-2427(93)90081-eGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a study that investigates the role of local antibodies in horses with chronic obstructive pulmonary disease (COPD). It indicates that elevated levels of certain specific antibodies in the bronchoalveolar lavage fluid (BALF), not seen in serum, of these horses potentially implicates these antibodies in the development and course of this disease.

Objective of the Research

  • The primary aim of this research was to understand the involvement of specific types of antibodies to the allergens Micropolyspora faeni and Aspergillus fumigatus in horses suffering from COPD and other respiratory diseases.

Methodology

  • An enzyme-linked immunosorbent assay (ELISA) was employed to measure isotype-specific antibody levels in sera and BALF of horses categorized into: healthy, suffering from COPD, and ones with other chronic respiratory ailments.
  • The variations in antibody levels were then compared across these different groups.

Key Findings

  • Higher levels of IgE and IgA antibodies to the allergens were identified in the BALF from horses affected by COPD, both when conditions were symptomatic and asymptomatic. These antibody levels did not show an increase in serum.
  • The increase in antibodies was also noted in horses that developed chronic cough following symptoms corresponding to a respiratory viral infection.
  • In an observed case, the IgE antibody level to both allergens increased tenfold over a period of seven weeks.

Implications of the Research

  • The results lend support to the hypothesis that local IgE antibodies reacting to mold allergens play a crucial role in the pathogenesis of COPD in horses.
  • The data also indicate that respiratory viral infections could make horses more susceptible to developing COPD.

In conclusion, this research deepens the understanding of COPD progression in horses and implies that controlling local antibodies could potentially be a therapeutic strategy.

Cite This Article

APA
Halliwell RE, McGorum BC, Irving P, Dixon PM. (1993). Local and systemic antibody production in horses affected with chronic obstructive pulmonary disease. Vet Immunol Immunopathol, 38(3-4), 201-215. https://doi.org/10.1016/0165-2427(93)90081-e

Publication

Veterinary immunology and immunopathology
ISSN: 0165-2427
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 38
Issue: 3-4
Pages: 201-215

Researcher Affiliations

Halliwell, R E
  • University of Edinburgh, Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, Roslin, UK.
McGorum, B C
    Irving, P
      Dixon, P M

        MeSH Terms

        • Animals
        • Antibodies, Bacterial / biosynthesis
        • Antibodies, Fungal / biosynthesis
        • Aspergillosis / immunology
        • Aspergillosis / veterinary
        • Aspergillus fumigatus / immunology
        • Bacterial Infections / immunology
        • Bacterial Infections / veterinary
        • Horse Diseases / immunology
        • Horse Diseases / microbiology
        • Horses
        • Lung Diseases, Obstructive / immunology
        • Lung Diseases, Obstructive / microbiology
        • Lung Diseases, Obstructive / veterinary
        • Micromonosporaceae / immunology

        Citations

        This article has been cited 21 times.
        1. Wyler M, Sage SE, Marti E, White S, Gerber V. Protein microarray allergen profiling in bronchoalveolar lavage fluid and serum of horses with asthma. J Vet Intern Med 2023 Jan;37(1):328-337.
          doi: 10.1111/jvim.16600pubmed: 36479920google scholar: lookup
        2. Klier J, Fuchs S, Winter G, Gehlen H. Inhalative Nanoparticulate CpG Immunotherapy in Severe Equine Asthma: An Innovative Therapeutic Concept and Potential Animal Model for Human Asthma Treatment. Animals (Basel) 2022 Aug 16;12(16).
          doi: 10.3390/ani12162087pubmed: 36009677google scholar: lookup
        3. Di Pietro R, Dubuc V, Manguin E, Giroux-Lafond R, Bédard C, Boivin R, Lavoie JP, Vesper SJ, Leclere M. Characterization of fungal exposure and dectin-1 expression in healthy horses and horses with severe asthma. Am J Vet Res 2022 May 8;83(6).
          doi: 10.2460/ajvr.21.09.0143pubmed: 35524958google scholar: lookup
        4. Lo Feudo CM, Stucchi L, Alberti E, Conturba B, Zucca E, Ferrucci F. Intradermal Testing Results in Horses Affected by Mild-Moderate and Severe Equine Asthma. Animals (Basel) 2021 Jul 13;11(7).
          doi: 10.3390/ani11072086pubmed: 34359214google scholar: lookup
        5. White SJ, Moore-Colyer M, Marti E, Hannant D, Gerber V, Coüetil L, Richard EA, Alcocer M. Antigen array for serological diagnosis and novel allergen identification in severe equine asthma. Sci Rep 2019 Oct 23;9(1):15170.
          doi: 10.1038/s41598-019-51820-7pubmed: 31645629google scholar: lookup
        6. White S, Moore-Colyer M, Marti E, Coüetil L, Hannant D, Richard EA, Alcocer M. Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma. J Vet Intern Med 2019 Sep;33(5):2327-2335.
          doi: 10.1111/jvim.15564pubmed: 31429513google scholar: lookup
        7. Klier J, Geis S, Steuer J, Geh K, Reese S, Fuchs S, Mueller RS, Winter G, Gehlen H. A comparison of nanoparticullate CpG immunotherapy with and without allergens in spontaneously equine asthma-affected horses, an animal model. Immun Inflamm Dis 2018 Mar;6(1):81-96.
          doi: 10.1002/iid3.198pubmed: 29094511google scholar: lookup
        8. Mir IA, Kumar B, Taku A, Faridi F, Bhat MA, Baba NA, Maqbool T. Bacteriological and Molecular Detection of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus in Equines of Northern India. J Equine Sci 2013;24(3):53-5.
          doi: 10.1294/jes.24.53pubmed: 24834002google scholar: lookup
        9. Sarmiento J, Perez B, Morales N, Henriquez C, Vidal L, Folch H, Galecio JS, Morán G. Apoptotic effects of tamoxifen on leukocytes from horse peripheral blood and bronchoalveolar lavage fluid. Vet Res Commun 2013 Dec;37(4):333-8.
          doi: 10.1007/s11259-013-9571-0pubmed: 23846832google scholar: lookup
        10. Moran G, Folch H, Henriquez C, Ortloff A, Barria M. Reaginic antibodies from horses with recurrent airway obstruction produce mast cell stimulation. Vet Res Commun 2012 Dec;36(4):251-8.
          doi: 10.1007/s11259-012-9534-xpubmed: 23011757google scholar: lookup
        11. Racine J, Gerber V, Feutz MM, Riley CP, Adamec J, Swinburne JE, Couetil LL. Comparison of genomic and proteomic data in recurrent airway obstruction affected horses using Ingenuity Pathway Analysis®. BMC Vet Res 2011 Aug 15;7:48.
          doi: 10.1186/1746-6148-7-48pubmed: 21843342google scholar: lookup
        12. Moran G, Buechner-Maxwell VA, Folch H, Henriquez C, Galecio JS, Perez B, Carrasco C, Barria M. Increased apoptosis of CD4 and CD8 T lymphocytes in the airways of horses with recurrent airway obstruction. Vet Res Commun 2011 Oct;35(7):447-56.
          doi: 10.1007/s11259-011-9482-xpubmed: 21594641google scholar: lookup
        13. Morán G, Folch H, Araya O, Burgos R, Barria M. Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay. Vet Res Commun 2010 Dec;34(8):719-26.
          doi: 10.1007/s11259-010-9440-zpubmed: 20814741google scholar: lookup
        14. Morán G, Burgos R, Araya O, Folch H. In vitro bioassay to detect reaginic antibodies from the serum of horses affected with recurrent airway obstruction. Vet Res Commun 2010 Jan;34(1):91-9.
          doi: 10.1007/s11259-009-9334-0pubmed: 20012188google scholar: lookup
        15. McAleese SM, Miller HR. Cloning and sequencing of the equine and ovine high-affinity IgE receptor beta-and gamma-chain cDNA. Immunogenetics 2003 May;55(2):122-5.
          doi: 10.1007/s00251-003-0564-ypubmed: 12709836google scholar: lookup
        16. Venugopalan CS, Beadle RE, Seahorn TL, Holmes EP. Responses of guinea-pig lung parenchymal strips to tracheobronchial lavage fluid from horses affected with summer pasture-associated obstructive pulmonary disease. Vet Res Commun 1998 Nov;22(7):493-503.
          doi: 10.1023/a:1006131217140pubmed: 9868763google scholar: lookup
        17. Jentsch MC, Keilhaue A, Wagner B, Rhyner C, Lübke S, Karagulyan M, Arnold C, Lohmann KL, Schnabel CL. Aspergillus fumigatus binding IgA and IgG1 are increased in bronchoalveolar lavage fluid of horses with neutrophilic asthma. Front Immunol 2024;15:1406794.
          doi: 10.3389/fimmu.2024.1406794pubmed: 38953030google scholar: lookup
        18. Jentsch MC, Lübke S, Schrödl W, Volke D, Krizsan A, Hoffmann R, Kaiser-Thom S, Gerber V, Marti E, Wagner B, Schnabel CL. Immunoproteomics enable broad identification of new Aspergillus fumigatus antigens in severe equine asthma. Front Immunol 2024;15:1347164.
          doi: 10.3389/fimmu.2024.1347164pubmed: 38487534google scholar: lookup
        19. Dai F, Dalla Costa E, Minero M, Briant C. Does housing system affect horse welfare? The AWIN welfare assessment protocol applied to horses kept in an outdoor group-housing system: The 'parcours'. Anim Welf 2023;32:e22.
          doi: 10.1017/awf.2023.9pubmed: 38487418google scholar: lookup
        20. Schnabel CL, Jentsch MC, Lübke S, Kaiser-Thom S, Gerber V, Vrtala S, Huang HJ, Rhyner C, Wagner B, Hoffmann R, Volke D. Immunoproteomics reveal increased serum IgG3/5 binding to Dermatophagoides and yeast protein antigens in severe equine asthma in a preliminary study. Front Immunol 2023;14:1293684.
          doi: 10.3389/fimmu.2023.1293684pubmed: 38162673google scholar: lookup
        21. Rivolta AA, Bujold AR, Wilmarth PA, Phinney BS, Navelski JP, Horohov DW, Sanz MG. Comparison of the broncoalveolar lavage fluid proteomics between foals and adult horses. PLoS One 2023;18(9):e0290778.
          doi: 10.1371/journal.pone.0290778pubmed: 37669266google scholar: lookup
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