Localization of Bovine papillomavirus in equine sarcoids and inflammatory skin conditions of horses using laser microdissection and two forms of DNA amplification.
Abstract: Equine sarcoids are the most common tumor of horses. Bovine papillomavirus (BPV) has been suggested as the cause of sarcoids. Studies have shown that BPV is present in swabs or biopsies from nonsarcoid-bearing equine skin. Skin biopsies from a variety of different conditions and normal skin from horses with no reported history of sarcoids were examined by polymerase chain reaction (PCR) for the presence of BPV, which was found in all different types of skin conditions as well as normal skin. Forty-one out of 86 skin biopsies from horses without sarcoids were found to contain BPV DNA. Laser microdissection, followed by DNA amplification through both PCR and isothermal loop-mediated amplification, was performed on these 41 biopsies and on 70 additional BPV-positive sarcoid biopsies to localize the virus. Location of BPV DNA was different between sarcoid and nonsarcoid groups. Nonsarcoid skin biopsies were more likely to have BPV within intact or inflamed epidermis than sarcoids (P = 0.016 and P = 0.007, respectively). Areas of inflammation within the dermis and epidermis were more likely to contain BPV than in noninflamed areas (P = 0.008 and P = 0.009, respectively). Bovine papillomavirus was also found in the epidermis of all types of sarcoids examined, more frequently in occult sarcoids than in fibroblastic and nodular types (P = 0.03 and P = 0.01, respectively). Results suggest that BPV is commonly found in normal and inflamed equine skin, and it is likely an important predisposing factor in the development of sarcoids.
Publication Date: 2011-12-06 PubMed ID: 22362933DOI: 10.1177/1040638711425952Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates the presence and location of Bovine papillomavirus (BPV) in horse skin tumor called equine sarcoids and other skin conditions. Using laser microdissection and DNA amplification techniques, the researchers identified BPV in various types of skin conditions, suggesting that the virus might be a significant factor in the development of horse tumors.
Research Objectives and Methods
- The primary aim of this research was to study the presence and location of Bovine papillomavirus (BPV) in equine sarcoids, a common skin tumor in horses, and other skin conditions using polymerase chain reaction (PCR), laser microdissection, and isothermal loop-mediated DNA amplification methods.
- The researchers took skin biopsies from a variety of different conditions and normal skin from horses with no recorded history of sarcoids. They examined these biopsies using PCR to find the presence of BPV.
- Laser microdissection, followed by DNA amplification via PCR and isothermal loop-mediated amplification, was executed on 41 biopsies out of the 86 from horses free of sarcoids and on an additional 70 sarcoid biopsies that were confirmed BPV-positive. This was performed to localize the virus.
Research Findings
- The study found BPV in all different types of skin conditions and even normal skin.
- The researchers identified BPV DNA in 41 out of 86 skin biopsies from horses without sarcoids.
- The location of BPV DNA was varied between sarcoid and non-sarcoid groups. Non-sarcoid skin biopsies were more likely to contain BPV within intact or inflamed epidermis than those from sarcoids.
- Areas of inflammation within the dermis and epidermis were more likely to contain BPV than non-inflamed areas.
- BPV was also discovered in the epidermis of all kinds of sarcoids explored, more frequently in occult sarcoids than in fibroblastic and nodular types.
Conclusion
- The findings suggest that BPV is commonly present in both normal and inflamed equine skin.
- The study proposes that presence of BPV is likely an essential predisposing factor in the development of sarcoids in horses.
Cite This Article
APA
Wobeser BK, Hill JE, Jackson ML, Kidney BA, Mayer MN, Townsend HG, Allen AL.
(2011).
Localization of Bovine papillomavirus in equine sarcoids and inflammatory skin conditions of horses using laser microdissection and two forms of DNA amplification.
J Vet Diagn Invest, 24(1), 32-41.
https://doi.org/10.1177/1040638711425952 Publication
Researcher Affiliations
- Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. bruce.wobeser@usask.ca
MeSH Terms
- Animals
- Bovine papillomavirus 1 / genetics
- DNA, Viral / genetics
- Dermatitis / veterinary
- Dermatitis / virology
- Dermatologic Surgical Procedures
- Horse Diseases / virology
- Horses / virology
- Keratinocytes / virology
- Microdissection / methods
- Microdissection / veterinary
- Microscopy, Confocal / methods
- Microscopy, Confocal / veterinary
- Nucleic Acid Amplification Techniques / methods
- Nucleic Acid Amplification Techniques / veterinary
- Papillomavirus Infections / veterinary
- Papillomavirus Infections / virology
- Polymerase Chain Reaction / veterinary
- Sarcoidosis / veterinary
- Sarcoidosis / virology
- Skin / virology
- Skin Diseases, Viral / veterinary
- Skin Diseases, Viral / virology
Citations
This article has been cited 6 times.- Ricard RM, Burton J, Chow-Lockerbie B, Wobeser B. Detection of Chlamydia abortus in aborted chorioallantoises of horses from Western Canada.. J Vet Diagn Invest 2023 Jul;35(4):359-365.
- Munday JS, Orbell G, Fairley RA, Hardcastle M, Vaatstra B. Evidence from a Series of 104 Equine Sarcoids Suggests That Most Sarcoids in New Zealand Are Caused by Bovine Papillomavirus Type 2, although Both BPV1 and BPV2 DNA Are Detectable in around 10% of Sarcoids.. Animals (Basel) 2021 Oct 29;11(11).
- Koziy RV, Yoshimura S, Dickinson R, Rybicka JM, Moshynskyy I, Ngeleka M, Bracamonte JL, Simko E. Use of standard diagnostic techniques to determine eradication of infection in experimental equine septic arthritis.. Can J Vet Res 2019 Jan;83(1):24-33.
- Wilson AD, Hicks C. Both tumour cells and infiltrating T-cells in equine sarcoids express FOXP3 associated with an immune-supressed cytokine microenvironment.. Vet Res 2016 May 9;47(1):55.
- Martano M, Corteggio A, Restucci B, De Biase ME, Borzacchiello G, Maiolino P. Extracellular matrix remodeling in equine sarcoid: an immunohistochemical and molecular study.. BMC Vet Res 2016 Feb 2;12:24.
- Araldi RP, Melo TC, Diniz N, Mazzuchelli-de-Souza J, Carvalho RF, Beçak W, Stocco RC. Bovine papillomavirus clastogenic effect analyzed in comet assay.. Biomed Res Int 2013;2013:630683.
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