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Home / Equine Research Bank / Mol Biol Rep / Lower expression of the equine maternally imprinted gene IGF...
Molecular biology reports2022; 50(1); 185-192; doi: 10.1007/s11033-022-07937-6

Lower expression of the equine maternally imprinted gene IGF2R is related to the slow proliferation of hinny embryonic fibroblast in vitro.

Abstract: Proliferation of embryonic fibroblasts under the same cell culture conditions, hinny embryonic fibroblasts (HiEFs) was slower than horse embryonic fibroblast (HEFs), donkey embryonic fibroblasts (DEFs) and mule embryonic fibroblasts (MuEFs). The imprinted genes IGF2 and IGF2R are important for cell proliferation. Therefore, we investigated whether the slower proliferation of HiEFs is related to an aberrant gene expression of IGF2 or its receptors or genes influencing the expression of the IGF2 system. Results: Real-time polymerase chain reaction, immunofluorescence and cell starving experiment in HEFs, DEFs, MuEFs and HiEFs revealed that the slower proliferation of HiEF in vitro was related to its lower expression of IGF2R (P < 0.001). Moreover, quantification of allele-specific expression and bisulfate assay confirmed that in both MuEFs and HiEFs, IGF2R had normal maternal imprinting, implying that the imprint aberrant was not involved in the lower IGF2R expression in HiEFs. Conclusions: The reduction of IGF2R expression in HiEFs is associated with its slower proliferation in vitro.
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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Publication Date: 2022-11-01 PubMed ID: 36319787DOI: 10.1007/s11033-022-07937-6Google Scholar: Lookup
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Summary

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The research study demonstrates that the slower proliferation of hinny embryonic fibroblasts (cells derived from embryos of female donkeys and male horses, abbreviated as HiEFs) as compared to other equine embryonic fibroblasts, is associated with the lower expression of a specific gene called IGF2R.

Research Context

  • The study started with the observation that under the same cell culture conditions, the proliferation rate of HiEFs was slower than that of horse embryonic fibroblasts (HEFs), donkey embryonic fibroblasts (DEFs), and mule embryonic fibroblasts (MuEFs).
  • The researchers highlighted that two imprinted genes, IGF2 and IGF2R, play significant roles in cell proliferation. This led them to investigate a potential link between the slower proliferation of HiEFs and unusual expression of IGF2, its receptors, and other genes affecting the IGF2 system.

Research Method and Findings

  • The researchers utilized real-time polymerase chain reaction, a tool for amplifying DNA, immunofluorescence to visualize proteins, and cell starving experiments to study the expression of these genes in HEFs, DEFs, MuEFs, and HiEFs.
  • The results confirmed that the slower proliferation of HiEFs in vitro was associated with lower expression of the IGF2R gene.
  • Further analysis showed that both MuEFs and HiEFs had normal maternal imprinting for the IGF2R gene. Maternal imprinting is a biological process ensuring the gene is only expressed from the gene copy inherited from the mother. This result suggested that abnormal imprinting was not the reason for lower IGF2R expression in HiEFs.

Conclusion

  • The research concluded that lower expression of the IGF2R gene is linked to the slower proliferation rate of HiEFs in vitro.

Cite This Article

APA
Wang X, Asgenbaatar N, Shen Y, Yi M, Zhao B, Ren H, Davshilt T, Ulaangerel T, Wang M, Burenbaatar A, Tian S, Li B, Dugarjav M, Bou G. (2022). Lower expression of the equine maternally imprinted gene IGF2R is related to the slow proliferation of hinny embryonic fibroblast in vitro. Mol Biol Rep, 50(1), 185-192. https://doi.org/10.1007/s11033-022-07937-6

Publication

Molecular biology reports
ISSN: 1573-4978
NlmUniqueID: 0403234
Country: Netherlands
Language: English
Volume: 50
Issue: 1
Pages: 185-192

Researcher Affiliations

Wang, Xisheng
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Asgenbaatar, Nairag
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Shen, Yingchao
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Yi, Minna
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Zhao, Bilig
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Ren, Hong
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Davshilt, Toli
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Ulaangerel, Tseweendolmaa
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Wang, Min
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Burenbaatar, Als
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Tian, Shuyue
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Li, Bei
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China.
Dugarjav, Manglai
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China. dmanglai@163.com.
Bou, Gerelchimeg
  • College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, 010018, Hohhot, China. gerelchimeg@imau.edu.cn.

MeSH Terms

  • Animals
  • Horses / genetics
  • Genomic Imprinting
  • Receptor, IGF Type 2 / genetics
  • Receptor, IGF Type 2 / metabolism
  • Alleles
  • Cell Proliferation / genetics
  • Equidae / genetics
  • Equidae / metabolism
  • Fibroblasts / metabolism
  • DNA Methylation

Grant Funding

  • 2019ZD03 / Natural Science Foundation of Inner Mongolia Autonomous Region
  • 2019GG242 / the Research project on Applied Technology in Inner Mongolia Autonomous Region
  • BZCG202112 / Youth Fund Project of College of Animal Science, Inner Mongolia Agricultural University

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