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Melanocortin receptor variants with phenotypic effects in horse, pig, and chicken.
Abstract: The melanocortin system is of considerable interest in domestic animals because their energy metabolism and pigmentation have been under strong selection. This article reviews our work on MC1R variants in horse, pig, and chicken, as well as a study on MC4R polymorphism in the pig. The chestnut coat color in horses is caused by an MC1R missense mutation (S83F). In the pig, we have described seven MC1R alleles controlling four different coat color phenotypes (wild type, dominant black, black spotting, and recessive red). The most interesting allele is the one causing black spotting because it carries two causative mutations, a frameshift and a missense mutation. The frameshift mutation is somatically unstable, and the black spots reflect somatic reversion events restoring the reading frame. Classic genetics have established eight alleles at the Extended black locus in chicken, which is assumed to correspond to the Extension locus in mammals. We have analyzed the co-segregation of alleles at MC1R and Extended black using a red jungle fowl x White Leghorn intercross and provide compelling evidence that these loci are identical. A previous study indicated that a missense mutation (D298N) in pig MC4R has an effect on fatness, growth, and feed intake. We could not confirm this association using an intercross between the wild boar and Large White domestic pigs, but it is possible that our F(2) generation was too small to detect the rather modest effect reported for this polymorphism.
Publication Date: 2003-07-10 PubMed ID: 12851331DOI: 10.1111/j.1749-6632.2003.tb03195.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Review
Summary
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The research explores the role of melanocortin receptor (MC1R and MC4R) variants in determining energy metabolism and pigmentation, specifically coat color, in horses, pigs, and chickens. The study identified specific mutations responsible for varying pigmentation phenotypes, and also examined the impact of MC4R polymorphism on traits such as fatness and growth in pigs.
Research Methodology and Findings
The research involves studying the melanocortin system in three types of domestic animals:
- In horses, a specific MC1R missense mutation (S83F) was identified as responsible for the chestnut coat color.
- In pigs, the researchers described seven MC1R alleles that control four different coat color phenotypes, including wild type, dominant black, black spotting, and recessive red. Notably, the allele causing black spotting carries two contributing mutations, a frameshift and a missense mutation. This frameshift mutation is notably unstable at the somatic level, which determined the presence of black spots through somatic reversion events.
- In chickens, through the analysis of co-segregation of MC1R alleles and Extended black alleles using a cross between red jungle fowl and White Leghorn, it was found that these loci are identical. It was also deduced from previous genetics that there are eight alleles at the Extended black locus, which is assumed to correspond to the Extension locus in mammals.
MC4R Polymorphism in Pigs
The study also discusses MC4R polymorphism in pigs, as a previous study suggested that a missense mutation (D298N) in pig MC4R affected fatness, growth, and feed intake.
- The research team attempted to verify this association through an intercross study between the wild boar and Large White domestic pigs.
- However, they were unable to confirm this influence, suggesting the possibility that the sample size of their second filial (F2) generation may have been too small to detect the previously stated modest effect of this polymorphism.
In conclusion, the research offers valuable insights into the genetic factors influencing pigmentation and energy metabolism traits in domestic animals, while also noting areas where further research could clarify existing knowledge gaps, particularly concerning the impact of MC4R polymorphism in pigs.
Cite This Article
APA
Andersson L.
(2003).
Melanocortin receptor variants with phenotypic effects in horse, pig, and chicken.
Ann N Y Acad Sci, 994, 313-318.
https://doi.org/10.1111/j.1749-6632.2003.tb03195.x Publication
Researcher Affiliations
- Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden. leif.andersson@imbim.uu.se
MeSH Terms
- Alleles
- Animals
- Body Weight
- Chickens / physiology
- Eating
- Hair Color / genetics
- Horses / physiology
- Mutation
- Polymorphism, Genetic
- Receptors, Corticotropin / genetics
- Receptors, Corticotropin / metabolism
- Receptors, Melanocortin
- Swine / physiology
Citations
This article has been cited 24 times.- Zhou Q, Cao C, Zhang H, Liang Y, Zhang X, Kang Y, Fang W, Lan X, Li R, Pan C. Convergent changes in melanocortin receptor 1 gene are associated with black-headed coat color in sheep. J Anim Sci 2023 Jan 3;101.
- Jiang XX, Adeola AC, Sola-Ojo FE, Abubakar IA, Fatima IH, Olaoluwa OJ, Abodurin AB, Olasunkanmi OA, Abisola OH, Uthman O, Kehinde AE, Hamidat H, Nishola TE, Bello SF, Peng MS, Zhang YP. Association of MC1R variation and plumage color diversity of Nigerian domestic pigeon (Columba livia domestica). J Adv Vet Anim Res 2022 Sep;9(3):369-373.
- Zhong H, Zhang J, Tan C, Shi J, Yang J, Cai G, Wu Z, Yang H. Pig Coat Color Manipulation by MC1R Gene Editing. Int J Mol Sci 2022 Sep 8;23(18).
- Alshanbari F, Castaneda C, Juras R, Hillhouse A, Mendoza MN, Gutiérrez GA, Ponce de León FA, Raudsepp T. Comparative FISH-Mapping of MC1R, ASIP, and TYRP1 in New and Old World Camelids and Association Analysis With Coat Color Phenotypes in the Dromedary (Camelus dromedarius). Front Genet 2019;10:340.
- Thomas AC, Heux P, Santos C, Arulvasan W, Solanky N, Carey ME, Gerrelli D, Kinsler VA, Etchevers HC. Widespread dynamic and pleiotropic expression of the melanocortin-1-receptor (MC1R) system is conserved across chick, mouse and human embryonic development. Birth Defects Res 2018 Mar 15;110(5):443-455.
- Mahmoud AH, Mashaly AM, Rady AM, Al-Anazi KM, Saleh AA. Allelic variation of melanocortin-1 receptor locus in Saudi indigenous sheep exhibiting different color coats. Asian-Australas J Anim Sci 2017 Feb;30(2):154-159.
- Jin S, Lee JH, Seo DW, Cahyadi M, Choi NR, Heo KN, Jo C, Park HB. A Major Locus for Quantitatively Measured Shank Skin Color Traits in Korean Native Chicken. Asian-Australas J Anim Sci 2016 Nov;29(11):1555-1561.
- Lü MD, Han XM, Ma YF, Irwin DM, Gao Y, Deng JK, Adeola AC, Xie HB, Zhang YP. Genetic variations associated with six-white-point coat pigmentation in Diannan small-ear pigs. Sci Rep 2016 Jun 8;6:27534.
- Chandramohan B, Renieri C, La Manna V, La Terza A. The alpaca melanocortin 1 receptor: gene mutations, transcripts, and relative levels of expression in ventral skin biopsies. ScientificWorldJournal 2015;2015:265751.
- Ramos-Onsins SE, Burgos-Paz W, Manunza A, Amills M. Mining the pig genome to investigate the domestication process. Heredity (Edinb) 2014 Dec;113(6):471-84.
- Yang GL, Fu DL, Lang X, Wang YT, Cheng SR, Fang SL, Luo YZ. Mutations in MC1R gene determine black coat color phenotype in Chinese sheep. ScientificWorldJournal 2013;2013:675382.
- Wang GD, Cheng LG, Fan RX, Irwin DM, Tang SS, Peng JG, Zhang YP. Signature of balancing selection at the MC1R gene in Kunming dog populations. PLoS One 2013;8(2):e55469.
- Bar I, Kaddar E, Velan A, David L. Melanocortin receptor 1 and black pigmentation in the Japanese ornamental carp (Cyprinus carpio var. Koi). Front Genet 2013;4:6.
- Hong LZ, Li J, Schmidt-Küntzel A, Warren WC, Barsh GS. Digital gene expression for non-model organisms. Genome Res 2011 Nov;21(11):1905-15.
- Pryce JE, Hayes BJ, Bolormaa S, Goddard ME. Polymorphic regions affecting human height also control stature in cattle. Genetics 2011 Mar;187(3):981-4.
- Ren J, Mao H, Zhang Z, Xiao S, Ding N, Huang L. A 6-bp deletion in the TYRP1 gene causes the brown colouration phenotype in Chinese indigenous pigs. Heredity (Edinb) 2011 May;106(5):862-8.
- Tao YX. The melanocortin-4 receptor: physiology, pharmacology, and pathophysiology. Endocr Rev 2010 Aug;31(4):506-43.
- Valenzano DR, Kirschner J, Kamber RA, Zhang E, Weber D, Cellerino A, Englert C, Platzer M, Reichwald K, Brunet A. Mapping loci associated with tail color and sex determination in the short-lived fish Nothobranchius furzeri. Genetics 2009 Dec;183(4):1385-95.
- Anderson TM, vonHoldt BM, Candille SI, Musiani M, Greco C, Stahler DR, Smith DW, Padhukasahasram B, Randi E, Leonard JA, Bustamante CD, Ostrander EA, Tang H, Wayne RK, Barsh GS. Molecular and evolutionary history of melanism in North American gray wolves. Science 2009 Mar 6;323(5919):1339-43.
- Alizadeh A, Hong LZ, Kaelin CB, Raudsepp T, Manuel H, Barsh GS. Genetics of Sex-linked yellow in the Syrian hamster. Genetics 2009 Apr;181(4):1427-36.
- Kerns JA, Cargill EJ, Clark LA, Candille SI, Berryere TG, Olivier M, Lust G, Todhunter RJ, Schmutz SM, Murphy KE, Barsh GS. Linkage and segregation analysis of black and brindle coat color in domestic dogs. Genetics 2007 Jul;176(3):1679-89.
- Cheviron ZA, Hackett SJ, Brumfield RT. Sequence variation in the coding region of the melanocortin-1 receptor gene (MC1R) is not associated with plumage variation in the blue-crowned manakin (Lepidothrix coronata). Proc Biol Sci 2006 Jul 7;273(1594):1613-8.
- Baldan S, Sölkner J, Gebre KT, Mészáros G, Crooijmans R, Periasamy K, Pichler R, Manaljav B, Baatar N, Purevdorj M. Genetic characterization of cashmere goat (Capra hircus) populations in Mongolia. Front Genet 2024;15:1421529.
- Bacon EK, Donnelly CG, Bellone RR, Haase B, Finno CJ, Velie BD. Preliminary investigation of potential links between pigmentation variants and opioid analgesic effectiveness in horses during cerebrospinal fluid centesis. BMC Vet Res 2024 Jul 12;20(1):311.
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