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Home / Equine Research Bank / J Vet Intern Med / Microarray molecular mapping of horses with severe asthma.
Journal of veterinary internal medicine2023; doi: 10.1111/jvim.16951

Microarray molecular mapping of horses with severe asthma.

Abstract: Severe asthma (SA) in horses, resembling human asthma, is a prevalent, debilitating allergic respiratory condition marked by elevated allergen-specific immunoglobulin E (IgE) against environmental proteins; however, research exploring the exposome's influence on IgE profiles is currently limited but holds paramount significance for diagnostic and therapeutic developments. Methods: Thirty-five sports horses were analyzed, consisting of environmentally matched samples from France (5 SA; 6 control), the United States (6 SA; 6 control), and Canada (6 SEA; 6 control). Methods: This intentional cross-sectional study investigated the sensitization profiles of SA-affected and healthy horses via serological antigen microarray profiling. Partial least square-discriminant analysis (PLS-DA) was used to identify and rank the importance of allergens for class separation (ie, affected/non-affected) as variable influence of projection (VIP), and allergen with commonality internationally established via frequency analysis. Results: PLS-DA models showed high discriminatory power in predicting SA in horses from Canada (area under the curve [AUC] 0.995) and France (AUC 0.867) but poor discriminatory power in horses from the United States (AUC 0.38). Hev b 5.0101, Cyn D, Der p 2, and Rum cr were the only shared allergens across all geographical groups. Conclusions: Microarray profiling can identify specific allergenic components associated with SA in horses, while mathematical modeling of this data can be used for disease classification, highlighting the variability of sensitization profiles between geographical locations and emphasizing the importance of local exposure to the prevalence of different allergens. Frequency scoring analysis can identify important variables that contribute to the classification of SA across different geographical regions.
© 2023 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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Publication Date: 2023-12-10 PubMed ID: 38071496DOI: 10.1111/jvim.16951Google Scholar: Lookup
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  • Journal Article

Summary

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The research article discusses how a technique known as microarray molecular mapping was used to study severe asthma in horses and to identify specific allergenic components associated with the disease, across different geographical regions.

Methodology

  • Thirty-five sports horses from France, the United States, and Canada participated in the study. Each geographical group included both horses with severe asthma (SA) and control horses without the disease. The horses were intentionally chosen to represent a cross-section of the population.
  • The researchers used a technique called serological antigen microarray profiling. This involves exposing a small sample of a patient’s serum to a microarray – a grid of thousands of microscopic spots, each containing a different potential allergen. If the patient has antibodies against a particular allergen, they will bind to it on the microarray, allowing the allergen to be identified.
  • The researchers used a statistical technique known as partial least square-discriminant analysis (PLS-DA) to process the results from the microarray. This technique can identify and rank the allergens that are most important for distinguishing between affected and non-affected individuals, according to their variable influence of projection.

Results

  • The PLS-DA models successfully distinguished between horses with SA and control horses in Canada and France. They were less successful in the United States.
  • The models identified four allergens that were common across all geographical groups: Hev b 5.0101, Cyn D, Der p 2, and Rum cr.
  • However, the sensitivity of horses to different allergens varied between geographical locations, underlining the influence of local exposure on allergic responses.

Conclusions

  • The results show that microarray profiling can identify specific allergens associated with severe asthma in horses.
  • Mathematical modelling of the results can help classify the disease and understand how it varies in different locations.
  • Identifying these allergy-triggering compounds may contribute to improved diagnosis and treatment of severe asthma in horses.

Cite This Article

APA
White SJ, Couetil L, Richard EA, Marti E, Wilson PB. (2023). Microarray molecular mapping of horses with severe asthma. J Vet Intern Med. https://doi.org/10.1111/jvim.16951

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English

Researcher Affiliations

White, Samuel J
  • Nottingham Trent University, Nottinghamshire, UK.
Couetil, Laurent
  • Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA.
Richard, Eric A
  • LABÉO Frank Duncombe, Caen Cedex, France.
  • Normandie Univ, UniCaen, BIOTARGEN, Saint-Contest, France.
Marti, Eliane
  • Department of Clinical Research and Veterinary Public Health, University of Bern, Bern, Switzerland.
Wilson, Philippe B
  • Nottingham Trent University, Nottinghamshire, UK.

Grant Funding

  • Medical Technologies Innovation Facility, Nottingham Trent University

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