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Endoscopic scoring of mucus quantity and quality: observer and horse variance and relationship to inflammation, mucus viscoelasticity and volume.
Abstract: Endoscopic scoring of airway mucus quantity and quality has not been critically assessed. Objective: To evaluate mucus scores for 1) observer- and horse-related variance and 2) association with inflammation, mucus viscoelasticity and measured volume. Methods: Variance of scoring within and between observers and over time within horses were determined for airway mucus accumulation, apparent viscosity, localisation and colour, and correlations of mucus accumulation scores with neutrophil ratios in secretions. The relationship of accumulation score to measured volumes of 'artificial mucus' was investigated. Correlations of mucus accumulation, apparent viscosity and colour scores with measured viscoelasticity were tested. Viscoelasticity was compared between tracheal secretion samples collected ventrally and dorsally. Results: Mucus accumulation scoring showed excellent interobserver agreement and moderate horse-related variance, was related to measured volumes of 'artificial mucus', and correlated well with neutrophilic airway inflammation. Scores of mucus viscosity, colour and localisation showed high observer-related variance. Mucus accumulation, apparent viscosity and colour scores did not correlate with measured tracheal mucus viscoelasticity, but dorsally-localised mucus showed 2-fold higher measured viscoelasticity than ventrally-localised samples. Conclusions: Mucus accumulation scores are a reproducible measure of mucus volumes in the trachea. Conclusions: Endoscopic scoring of mucus accumulation is a reliable clinical and research tool. In contrast, apparent viscosity, localisation and colour scores should be interpreted with caution.
Publication Date: 2004-12-08 PubMed ID: 15581321DOI: 10.2746/0425164044864525Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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This research analyzes the effectiveness and reliability of endoscopic scoring for airway mucus. The study reveals that mucus accumulation scores are consistently reproducible and hence, reliable. However, scores for apparent viscosity, mucus placement, and color display high variance and should be interpreted with caution.
Objective and Methodology
- The primary objective of the research is to evaluate the interobserver and horse-related variance in mucus scores and to assess its relationship with inflammation, mucus viscoelasticity, and measured volume.
- The researchers determined the variance of scoring within and between observers and over time within horses for airway mucus accumulation, apparent viscosity, localization, and color.
- The correlation between mucus accumulation scores and neutrophil ratios in secretions was investigated, along with the relationship of accumulation score to the measured volumes of ‘artificial mucus’.
- The research then tested correlations of mucus accumulation, apparent viscosity and color scores with the measured viscoelasticity.
- Finally, the viscoelasticity was compared between tracheal secretion samples collected ventrally and dorsally.
Results and Conclusion
- The study confirms that mucus accumulation scoring demonstrates superior agreement among different observers and moderate horse-related variance. Furthermore, it has a robust relationship with the measured volumes of ‘artificial mucus’ and aligns well with neutrophilic airway inflammation.
- Contrarily, the scores for mucus viscosity, color, and localization demonstrated high observer-related variance, which questions their reliability.
- Mucus accumulation, apparent viscosity, and color scores could not establish a strong correlation with tracheal mucus viscoelasticity. However, it was found that mucus localized dorsally had 2-fold higher measured viscoelasticity than ventrally-localized samples.
- In conclusion, while mucus accumulation scores offer a reliable measure of mucus volumes in trachea, scarcity of uniformity in scoring for apparent viscosity, localization, and color requires these parameters to be interpreted with caution. The research underscores the value of endoscopic scoring of mucus accumulation as a reliable tool in clinical and research settings.
Cite This Article
APA
Gerber V, Straub R, Marti E, Hauptman J, Herholz C, King M, Imhof A, Tahon L, Robinson NE.
(2004).
Endoscopic scoring of mucus quantity and quality: observer and horse variance and relationship to inflammation, mucus viscoelasticity and volume.
Equine Vet J, 36(7), 576-582.
https://doi.org/10.2746/0425164044864525 Publication
Researcher Affiliations
- Pulmonary Laboratory, Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, E Lansing, USA.
MeSH Terms
- Animals
- Endoscopy / veterinary
- Horse Diseases / diagnosis
- Horse Diseases / pathology
- Horses
- Inflammation / diagnosis
- Inflammation / pathology
- Inflammation / veterinary
- Mucus / metabolism
- Mucus / physiology
- Observer Variation
- Pigmentation
- Respiratory Tract Diseases / diagnosis
- Respiratory Tract Diseases / pathology
- Respiratory Tract Diseases / veterinary
- Viscosity
Citations
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