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Respiratory metabolites in bronchoalveolar lavage fluid (BALF) and exhaled breath condensate (EBC) can differentiate horses affected by severe equine asthma from healthy horses.
Abstract: The use of an untargeted metabolomic approach to investigate biofluids of respiratory origin is of increasing interest in human and veterinary lung research. Considering the high incidence of equine asthma (> 14%) within horse population and the importance of this animal model for human disease, we aimed to investigate the metabolomic profile of bronchoalveolar lavage fluid (BALF) and exhaled breath condensate (EBC) in healthy and asthmatic horses. Results: On the basis of clinical, endoscopic and BALF cytology findings, 6 horses with severe asthma (Group A) and 6 healthy horses (Group C) were included in the study. H-NMR analysis was used to identified metabolites in BALF and EBC samples. Metabolomic analysis allowed to identify and quantify 12 metabolites in BALF and seven metabolites in EBC. Among respiratory metabolites, myo-inositol, formate, glycerol and isopropanol in BALF, and methanol and ethanol in EBC, differed between groups (p < 0.05). Conclusions: The application of metabolomic studies to investigate equine asthma using minimally invasive diagnostic methods, such as EBC metabolomics, provided promising results. According to our research, the study of selective profiles of BALF and EBC metabolites might be useful for identifying molecules like myo-inositol and methanol as possible biomarkers for airways diseases in horses.
Publication Date: 2020-07-08 PubMed ID: 32641035PubMed Central: PMC7346432DOI: 10.1186/s12917-020-02446-9Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Animal Health
- Asthma
- Biomarkers
- Bronchoalveolar Lavage
- Clinical Findings
- Clinical Study
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Disease Management
- Disease Treatment
- Equine Diseases
- Equine Health
- Horses
- Metabolites
- Metabolomics
- Respiratory Disease
- Respiratory Health
- Veterinary Medicine
- Veterinary Research
Summary
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This research investigates the metabolic profile of bronchoalveolar lavage fluid (BALF) and exhaled breath condensate (EBC) in healthy and asthmatic horses, examining whether differences can serve as a diagnostic tool for equine asthma.
Research Purpose
- The study aims to identify differences in the metabolomic profiles of healthy horses versus those with severe asthma. The intent is to discover potential biomarkers that could help diagnose and treat equine asthma, which affects over 14% of the horse population.
- By studying the metabolomic profile of the respiratory biofluids like BALF and EBC, the researchers were seeking potential non-invasive diagnostic methods for respiratory diseases in horses, and possibly extrapolate the insights for human disease research.
Methodology
- The study included 6 healthy horses (Group C) and 6 horses with severe asthma (Group A), classified based on clinical, endoscopic, and BALF cytology findings.
- Both BALF and EBC samples were collected from the horses and analyzed using H-NMR to identify and quantify the metabolic compounds present.
Results
- The metabolomic analysis identified and quantified 12 metabolites in BALF and 7 metabolites in EBC.
- Among the various identified metabolites, myo-inositol, formate, glycerol, and isopropanol in BALF, as well as methanol and ethanol in EBC showed significant differences between the healthy horses and those with severe asthma.
Conclusions
- The research concludes that metabolomic studies of BALF and EBC can provide valuable insights in the diagnosis and management of respiratory diseases like equine asthma.
- Specifically, the study highlights myo-inositol and methanol as possible biomarkers for respiratory diseases in horses, indicating a promising potential for the use of non-invasive diagnostic methods.
Cite This Article
APA
Bazzano M, Laghi L, Zhu C, Magi GE, Tesei B, Laus F.
(2020).
Respiratory metabolites in bronchoalveolar lavage fluid (BALF) and exhaled breath condensate (EBC) can differentiate horses affected by severe equine asthma from healthy horses.
BMC Vet Res, 16(1), 233.
https://doi.org/10.1186/s12917-020-02446-9 Publication
Researcher Affiliations
- School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024, Matelica, MC, Italy. marilena.bazzano@unicam.it.
- Department of Agro-Food Science and Technology, Centre of Foodomics, University of Bologna, Bologna, Italy.
- Department of Agro-Food Science and Technology, Centre of Foodomics, University of Bologna, Bologna, Italy.
- School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024, Matelica, MC, Italy.
- School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024, Matelica, MC, Italy.
- School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024, Matelica, MC, Italy.
MeSH Terms
- Animals
- Asthma / diagnosis
- Asthma / veterinary
- Biomarkers / analysis
- Breath Tests
- Bronchoalveolar Lavage Fluid / chemistry
- Horse Diseases / diagnosis
- Horses
- Lung / chemistry
Conflict of Interest Statement
The authors declare that they have no competing interests.
References
This article includes 52 references
- Bozzetto S, Pirillo P, Carraro S, Berardi M, Cesca L, Stocchero M, Giordano G, Zanconato S, Baraldi E. Metabolomic profile of children with recurrent respiratory infections.. Pharmacol Res 2017 Jan;115:162-167.
- Nambiar S, Bong How S, Gummer J, Trengove R, Moodley Y. Metabolomics in chronic lung diseases. Respirology. 2019;2019.
- Karampitsakos T, Gourgoulianis KI. Asthma-COPD Overlap Syndrome (ACOS): Single disease entity or not? Could exhaled nitric oxide be a useful biomarker for the differentiation of ACOS, asthma and COPD?. Med Hypotheses 2016 Jun;91:20-23.
- Kao CC, Hsu JW, Bandi V, Hanania NA, Kheradmand F, Jahoor F. Glucose and pyruvate metabolism in severe chronic obstructive pulmonary disease.. J Appl Physiol (1985) 2012 Jan;112(1):42-7.
- Wolak JE, Esther CR Jr, O'Connell TM. Metabolomic analysis of bronchoalveolar lavage fluid from cystic fibrosis patients.. Biomarkers 2009 Feb;14(1):55-60.
- Kang YP, Lee SB, Lee JM, Kim HM, Hong JY, Lee WJ, Choi CW, Shin HK, Kim DJ, Koh ES, Park CS, Kwon SW, Park SW. Metabolic Profiling Regarding Pathogenesis of Idiopathic Pulmonary Fibrosis.. J Proteome Res 2016 May 6;15(5):1717-24.
- Jones OA, Cheung VL. An introduction to metabolomics and its potential application in veterinary science.. Comp Med 2007 Oct;57(5):436-42.
- Zhu C, Faillace V, Laus F, Bazzano M, Laghi L. Characterization of trotter horses urine metabolome by means of proton nuclear magnetic resonance spectroscopy.. Metabolomics 2018 Aug 3;14(8):106.
- Laghi L, Zhu C, Campagna G, Rossi G, Bazzano M, Laus F. Probiotic supplementation in trained trotter horses: effect on blood clinical pathology data and urine metabolomic assessed in field.. J Appl Physiol (1985) 2018 Aug 1;125(2):654-660.
- Maniscalco M, Paris D, Melck DJ, Molino A, Carone M, Ruggeri P, Caramori G, Motta A. Differential diagnosis between newly diagnosed asthma and COPD using exhaled breath condensate metabolomics: a pilot study.. Eur Respir J 2018 Mar;51(3).
- Bazzano M, Laghi L, Zhu C, Magi GE, Serri E, Spaterna A, Tesei B, Laus F. Metabolomics of tracheal wash samples and exhaled breath condensates in healthy horses and horses affected by equine asthma.. J Breath Res 2018 Sep 27;12(4):046015.
- Barton AK, Gehlen H. Pulmonary Remodeling in Equine Asthma: What Do We Know about Mediators of Inflammation in the Horse?. Mediators Inflamm 2016;2016:5693205.
- Bullone M, Lavoie JP. Asthma "of horses and men"--how can equine heaves help us better understand human asthma immunopathology and its functional consequences?. Mol Immunol 2015 Jul;66(1):97-105.
- Bullone M, Lavoie JP. The Contribution of Oxidative Stress and Inflamm-Aging in Human and Equine Asthma.. Int J Mol Sci 2017 Dec 5;18(12).
- Kirschvink N, Reinhold P. Use of alternative animals as asthma models.. Curr Drug Targets 2008 Jun;9(6):470-84.
- Lu00e9guillette R. Recurrent airway obstruction--heaves.. Vet Clin North Am Equine Pract 2003 Apr;19(1):63-86, vi.
- Korn A, Miller D, Dong L, Buckles EL, Wagner B, Ainsworth DM. Differential Gene Expression Profiles and Selected Cytokine Protein Analysis of Mediastinal Lymph Nodes of Horses with Chronic Recurrent Airway Obstruction (RAO) Support an Interleukin-17 Immune Response.. PLoS One 2015;10(11):e0142622.
- Comhair SA, McDunn J, Bennett C, Fettig J, Erzurum SC, Kalhan SC. Metabolomic Endotype of Asthma.. J Immunol 2015 Jul 15;195(2):643-50.
- Gy C, Leclere M, Vargas A, Grimes C, Lavoie JP. Investigation of blood biomarkers for the diagnosis of mild to moderate asthma in horses.. J Vet Intern Med 2019 Jul;33(4):1789-1795.
- Duz M, Whittaker AG, Love S, Parkin TD, Hughes KJ. Exhaled breath condensate hydrogen peroxide and pH for the assessment of lower airway inflammation in the horse.. Res Vet Sci 2009 Oct;87(2):307-12.
- Rahman I. Reproducibility of oxidative stress biomarkers in breath condensate: are they reliable?. Eur Respir J 2004 Feb;23(2):183-4.
- Gerber V, Straub R, Marti E, Hauptman J, Herholz C, King M, Imhof A, Tahon L, Robinson NE. Endoscopic scoring of mucus quantity and quality: observer and horse variance and relationship to inflammation, mucus viscoelasticity and volume.. Equine Vet J 2004 Nov;36(7):576-82.
- Hodgson JL, Hodgson DR. Collection and analysis of respiratory tract samples. In: Equine Respiratory Medicine and Surgery. 2007. p. 119u201350.
- Bizzarri M, Fuso A, Dinicola S, Cucina A, Bevilacqua A. Pharmacodynamics and pharmacokinetics of inositol(s) in health and disease.. Expert Opin Drug Metab Toxicol 2016 Oct;12(10):1181-96.
- Gilmore AP, Burridge K. Regulation of vinculin binding to talin and actin by phosphatidyl-inositol-4-5-bisphosphate.. Nature 1996 Jun 6;381(6582):531-5.
- Hallman M, Spragg R, Harrell JH, Moser KM, Gluck L. Evidence of lung surfactant abnormality in respiratory failure. Study of bronchoalveolar lavage phospholipids, surface activity, phospholipase activity, and plasma myoinositol.. J Clin Invest 1982 Sep;70(3):673-83.
- Howlett A, Ohlsson A, Plakkal N. Inositol in preterm infants at risk for or having respiratory distress syndrome.. Cochrane Database Syst Rev 2019 Jul 8;7(7):CD000366.
- Phelps DL, Ward RM, Williams RL, Nolen TL, Watterberg KL, Oh W, Goedecke M, Ehrenkranz RA, Fennell T, Poindexter BB, Cotten CM, Hallman M, Frantz ID 3rd, Faix RG, Zaterka-Baxter KM, Das A, Ball MB, Lacy CB, Walsh MC, Carlo WA, Su00e1nchez PJ, Bell EF, Shankaran S, Carlton DP, Chess PR, Higgins RD. Safety and pharmacokinetics of multiple dose myo-inositol in preterm infants.. Pediatr Res 2016 Aug;80(2):209-17.
- Benesch R, Benesch RE. The effect of organic phosphates from the human erythrocyte on the allosteric properties of hemoglobin.. Biochem Biophys Res Commun 1967 Jan 23;26(2):162-7.
- Lam G, Zhao S, Sandhu J, Yi R, Loganathan D, Morrissey B. Detection of myo-inositol tris pyrophosphate (ITPP) in equine following an administration of ITPP.. Drug Test Anal 2014 Mar;6(3):268-76.
- Viswan A, Sharma RK, Azim A, Sinha N. NMR-Based Metabolic Snapshot from Minibronchoalveolar Lavage Fluid: An Approach To Unfold Human Respiratory Metabolomics.. J Proteome Res 2016 Jan 4;15(1):302-10.
- Benahmed MA, Elbayed K, Daubeuf F, Santelmo N, Frossard N, Namer IJ. NMR HRMAS spectroscopy of lung biopsy samples: Comparison study between human, pig, rat, and mouse metabolomics.. Magn Reson Med 2014 Jan;71(1):35-43.
- Evans CR, Karnovsky A, Kovach MA, Standiford TJ, Burant CF, Stringer KA. Untargeted LC-MS metabolomics of bronchoalveolar lavage fluid differentiates acute respiratory distress syndrome from health.. J Proteome Res 2014 Feb 7;13(2):640-9.
- Ho WE, Xu YJ, Xu F, Cheng C, Peh HY, Tannenbaum SR, Wong WS, Ong CN. Metabolomics reveals altered metabolic pathways in experimental asthma.. Am J Respir Cell Mol Biol 2013 Feb;48(2):204-11.
- Ho WE, Xu YJ, Cheng C, Peh HY, Tannenbaum SR, Wong WSF, Ong CN. Metabolomics Reveals Inflammatory-Linked Pulmonary Metabolic Alterations in a Murine Model of House Dust Mite-Induced Allergic Asthma.. J Proteome Res 2014 Aug 1;13(8):3771-3782.
- Brosnan ME, Brosnan JT. Formate: The Neglected Member of One-Carbon Metabolism.. Annu Rev Nutr 2016 Jul 17;36:369-88.
- Lamarre SG, Molloy AM, Reinke SN, Sykes BD, Brosnan ME, Brosnan JT. Formate can differentiate between hyperhomocysteinemia due to impaired remethylation and impaired transsulfuration.. Am J Physiol Endocrinol Metab 2012 Jan 1;302(1):E61-7.
- Katre P, Joshi S, Bhat DS, Deshmukh M, Gurav N, Pandit S, Lubree H, Marczewski S, Bennett C, Gruca L, Kalyanaraman K, Naik SS, Yajnik CS, Kalhan SC. Effect of multi-nutrient insufficiency on markers of one carbon metabolism in young women: response to a methionine load.. Eur J Clin Nutr 2016 Jun;70(6):687-93.
- Hong JH, Lee WC, Hsu YM, Liang HJ, Wan CH, Chien CL, Lin CY. Characterization of the biochemical effects of naphthalene on the mouse respiratory system using NMR-based metabolomics.. J Appl Toxicol 2014 Dec;34(12):1379-88.
- Maniscalco M, Paris D, Melck DJ, Molino A, Carone M, Ruggeri P, et al. Early View Differential diagnosis between newly diagnosed asthma and chronic obstructive pulmonary disease using exhaled breath condensate metabolomics: a pilot study. Eur Respir J. 2018:1825u20132017.
- Montuschi P, Paris D, Melck D, Lucidi V, Ciabattoni G, Raia V, Calabrese C, Bush A, Barnes PJ, Motta A. NMR spectroscopy metabolomic profiling of exhaled breath condensate in patients with stable and unstable cystic fibrosis.. Thorax 2012 Mar;67(3):222-8.
- Carraro S, Rezzi S, Reniero F, Hu00e9berger K, Giordano G, Zanconato S, Guillou C, Baraldi E. Metabolomics applied to exhaled breath condensate in childhood asthma.. Am J Respir Crit Care Med 2007 May 15;175(10):986-90.
- Carden KA, Boiselle PM, Waltz DA, Ernst A. Tracheomalacia and tracheobronchomalacia in children and adults: an in-depth review.. Chest 2005 Mar;127(3):984-1005.
- Laus F, Attili AR, Cerquetella M, Spaterna A, Tesei B, Cuteri V. Endoscopic findings, microbiological and cytological evaluation of tracheal aspirates in a population of Standardbred horses with poor performances. Vet Med (Praha) 2009;54:444u2013450.
- Couu00ebtil LL, Cardwell JM, Gerber V, Lavoie JP, Lu00e9guillette R, Richard EA. Inflammatory Airway Disease of Horses--Revised Consensus Statement.. J Vet Intern Med 2016 Mar-Apr;30(2):503-15.
- Pirie RS, Couu00ebtil LL, Robinson NE, Lavoie JP. Equine asthma: An appropriate, translational and comprehendible terminology?. Equine Vet J 2016 Jul;48(4):403-5.
- Hermange T, Le Corre S, Bizon C, Richard EA, Couroucu00e9 A. Bronchoalveolar lavage fluid from both lungs in horses: Diagnostic reliability of cytology from pooled samples.. Vet J 2019 Feb;244:28-33.
- Foschi C, Laghi L, D'Antuono A, Gaspari V, Zhu C, Dellarosa N, Salvo M, Marangoni A. Urine metabolome in women with Chlamydia trachomatis infection.. PLoS One 2018;13(3):e0194827.
- Dieterle F, Ross A, Schlotterbeck G, Senn H. Probabilistic quotient normalization as robust method to account for dilution of complex biological mixtures. Application in 1H NMR metabonomics.. Anal Chem 2006 Jul 1;78(13):4281-90.
- Box GEP, Cox DR. An analysis of transformations. J R Stat Soc Ser B. 1964;26:211u2013243.
- Harris JE, Boushey C, Bruemmer B, Archer SL. Publishing nutrition research: a review of nonparametric methods, part 3.. J Am Diet Assoc 2008 Sep;108(9):1488-96.
- Hubert M, Rousseeuw PJ, Vanden BK. ROBPCA: a new approach to robust principal component analysis. Technometrics. 2005;47:64u201379.
Citations
This article has been cited 3 times.- Simu00f5es J, Batista M, Tilley P. The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing.. Animals (Basel) 2022 Mar 16;12(6).
- Slowikowska M, Bajzert J, Miller J, Stefaniak T, Niedzwiedz A. The Dynamics of Circulating Immune Complexes in Horses with Severe Equine Asthma.. Animals (Basel) 2021 Apr 2;11(4).
- Bazzano M, Laghi L, Zhu C, Lotito E, Sgariglia S, Tesei B, Laus F. Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by (1)H-NMR.. Metabolites 2020 Jul 21;10(7).
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