Microquantitative determination of the distribution patterns of alcohol dehydrogenase activity in the liver of rat, guinea-pig and horse.
Abstract: Microquantitative measurements of ADH-activity were carried out on the livers of male and female rats, guinea-pigs and horses (two geldings and a mare). Lyophilized cryostat sections of liver parenchyma were microdissected the whole way along the sinusoidal length from the terminal afferent vessels to the terminal efferent venule. ADH activity in samples of about 50-150 ng was measured in a microbiochemical assay using the oil-well technique without enzymatic cycling, by direct luminometric determination of NADH. On the basis of the single measurements, mean values of total hepatic ADH activity could be calculated and the specific distribution patterns graphically demonstrated. Total activity of ADH in the liver of the female rat is 1.6 times higher than in the male; the male distribution pattern exhibits a relative maximum in the intermediary zone of the acinus while the activity in the liver of female rats increases towards a perivenous maximum. Mean values for total ADH activity in the livers of male and female guinea-pigs are almost equal and there is, moreover, no clear intra-acinar gradient. Mare and castrated male horses show high hepatic ADH activity which is evenly distributed in the liver acinus.
Publication Date: 1985-01-01 PubMed ID: 2934353DOI: 10.1007/BF00509205Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research is about an in-depth study on the distribution of alcohol dehydrogenase (ADH) enzyme in the livers of male and female rats, guinea-pigs, and horses.
Objective and Approach
- The study aimed to microquantitatively measure alcohol dehydrogenase (ADH) activity in the livers of male and female rats, guinea-pigs, and horses. It aimed to understand how ADH distribution varies between genders and species.
- For this purpose, they used the oil-well technique that did not involve enzymatic cycling, and NADH’s direct luminometric determination was made.
- Lyophilized cryostat sections of the liver were used for the detailed analysis, which were dissected along the sinusoidal length flanked by the terminal afferent vessels and the terminal efferent venule.
- Once the measurements were taken, mean values and specific distribution patterns were calculated and graphically presented.
Findings
- The absolute value of ADH activity in the liver of female rats was found to be 1.6 times higher compared to male rats. The distribution pattern in male rats showed a relative maximum in the intermediary zone of the acinus.
- ADH activity in the liver of female rats was found to increase towards a perivenous maximum.
- When observed in guinea-pigs, the total ADH activity in the livers of both males and females was nearly the same. Additionally, no clear intra-acinar gradient was noted in this case.
- Regardless of the gender, high hepatic ADH activity was seen in horses, evenly distributed within the liver acinus.
Significance
- The result of this research expands our understanding about the distribution of ADH activity in different species. This could be essential information for numerous studies, especially those dealing with liver diseases, metabolism study and the effects of alcohol.
- The differences in the distribution of ADH activity between genders could also be an important consideration in research design and clinical trials. Understanding these variations could guide hypotheses related to the sex differences in response to drugs or alcohol.
Cite This Article
APA
Maly IP, Sasse D.
(1985).
Microquantitative determination of the distribution patterns of alcohol dehydrogenase activity in the liver of rat, guinea-pig and horse.
Histochemistry, 83(5), 431-436.
https://doi.org/10.1007/BF00509205 Publication
Researcher Affiliations
MeSH Terms
- Alcohol Dehydrogenase
- Alcohol Oxidoreductases / analysis
- Animals
- Female
- Guinea Pigs
- Horses
- Liver / enzymology
- Male
- Rats
- Rats, Inbred Strains
- Sex Factors
- Species Specificity
References
This article includes 18 references
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