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Home / Equine Research Bank / Vet Res Commun / Modulatory effects of non-steroidal anti-inflammatory drugs ...
Veterinary research communications2011; 36(1); 29-33; doi: 10.1007/s11259-011-9507-5

Modulatory effects of non-steroidal anti-inflammatory drugs on the luminol and lucigenin amplified chemiluminescence of equine neutrophils.

Abstract: The purpose of this study was to explore the potential modulation of equine neutrophil oxidative burst by a series of classical NSAIDs which was subsequently monitored by the luminol or lucigenin-enhanced chemiluminescence (CL) technique. A significant dose-dependent inhibition of the luminol CL was observed with the majority of investigated drugs. This inhibition was very significant for phenylbutazone and Indomethacin; while for aspirin, a higher concentration is required. The action of Ketoprofen was significant during the first 5 min and only when the concentration was above 1 mM. Indomethacin and acetylsalicylic acid result in an inhibition dose-dependent of luminol CL. On the other hand, the phenylbutazone showed an inhibiting effect when used either luminol or lucigenin though luminol is slightly better. When the ketoprofen is considered, an inhibiting effect of luminal CL was observed but less significant than the other NSAIDs investigated. The flunixin meglumine enhances strongly the CL.
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Publication Date: 2011-10-21 PubMed ID: 22012073DOI: 10.1007/s11259-011-9507-5Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the impact of various non-steroidal anti-inflammatory drugs (NSAIDs) on the oxidative burst of horse white blood cells and their subsequent chemiluminescence response. The study found that different NSAIDs exhibit varied modulatory effects on the chemiluminescence of these cells.

Exploring the context

  • The research is centered on equine neutrophils, which are a type of white blood cell in horses.
  • These cells play a crucial role in the body’s immune response and their activity can be measured by the emission of light, known as chemiluminescence.
  • The study looks into how non-steroidal anti-inflammatory drugs (NSAIDs) – a class of drugs commonly used for relieving pain and inflammation – can affect the chemiluminescence of the neutrophils.
  • Specifically, this process is measured using two specific chemical substances: luminol and lucigenin. Both of these substances are capable of producing light when they react with certain chemicals.

Results of the study

  • Results showed that most of the NSAIDs investigated had a dose-dependent inhibitive effect on luminol chemiluminescence. This means that as the dose of the NSAID increased, the chemiluminescence decreased.
  • Two drugs, phenylbutazone and indomethacin, showed this effect to a very significant extent. However, for aspirin (acetylsalicylic acid), a higher dosage was required in order to observe this inhibition.
  • This inhibitive effect was notable with ketoprofen only during the first five minutes and only when the dose exceeded 1 mM.
  • Phenylbutazone showed an inhibiting effect regardless of whether the reaction was measured using luminol or lucigenin, but the effect was slightly stronger with luminol.
  • The drug flunixin meglumine, on the other hand, enhanced chemiluminescence instead of inhibiting it.

Significance of the findings

  • This research provides useful insights into how NSAIDs can modulate the oxidative burst response of equine neutrophils, a crucial aspect of the body’s immune response.
  • The findings may help in developing effective therapeutic approaches for horse health care, especially in managing inflammatory conditions.

Cite This Article

APA
Benbarek H, Ayad A, Deby-Dupont G, Boukraa L, Serteyn D. (2011). Modulatory effects of non-steroidal anti-inflammatory drugs on the luminol and lucigenin amplified chemiluminescence of equine neutrophils. Vet Res Commun, 36(1), 29-33. https://doi.org/10.1007/s11259-011-9507-5

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 36
Issue: 1
Pages: 29-33

Researcher Affiliations

Benbarek, H
  • University of Mascara, 29000, Mascara, Algeria. benbarekh@yahoo.com
Ayad, A
    Deby-Dupont, G
      Boukraa, L
        Serteyn, D

          MeSH Terms

          • Acridines / chemistry
          • Animals
          • Anti-Inflammatory Agents, Non-Steroidal / pharmacology
          • Dose-Response Relationship, Drug
          • Horses / immunology
          • Luminescent Measurements / veterinary
          • Luminol / chemistry
          • Neutrophils / drug effects
          • Neutrophils / metabolism
          • Oxidation-Reduction
          • Respiratory Burst / drug effects

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