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Home / Equine Research Bank / Infect Immun / Molecular characterization of equine isolates of Streptococc...
Infection and immunity1999; 67(6); 2776-2782; doi: 10.1128/IAI.67.6.2776-2782.1999

Molecular characterization of equine isolates of Streptococcus pneumoniae: natural disruption of genes encoding the virulence factors pneumolysin and autolysin.

Abstract: Although often considered a strict human pathogen, Streptococcus pneumoniae has been reported to infect and cause pneumonia in horses, although the pathology appears restricted compared to that of human infections. Here we report on the molecular characterization of a group of S. pneumoniae isolates obtained from horses in England and Ireland. Despite being obtained from geographically distinct locations, the isolates were found to represent a tight clonal group, virtually identical to each other but genetically distinguishable from more than 120 divergent isolates of human S. pneumoniae. A comprehensive analysis of known pneumococcal virulence determinants was undertaken in an attempt to understand the pathogenicity of equine pneumococci. Surprisingly, equine isolates appear to lack activities associated with both the hemolytic cytotoxin pneumolysin, often considered a major virulence factor of pneumococci, and the major autolysin gene lytA, also considered an important virulence factor. In support of phenotypic data, molecular studies demonstrated a deletion of parts of the coding sequences of both lytA and ply genes in equine pneumococci. The implications of these findings for the evolution and pathogenicity of equine S. pneumoniae are discussed.
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Publication Date: 1999-05-25 PubMed ID: 10338480PubMed Central: PMC96581DOI: 10.1128/IAI.67.6.2776-2782.1999Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study entails the molecular examination of Streptococcus pneumoniae (a bacterial pathogen) isolates in horses from England and Ireland. Despite the different locations, the isolates were genetically similar to each other but varied from those found in human variants of the bacteria. Interestingly, these equine isolates also seemed deficient in pneumolysin and autolysin, activity, two commonly considered virulence factors in Streptococcus pneumoniae.

Findings on Different Strains of S. pneumoniae

  • The paper presents an in-depth analysis of S. pneumoniae strains isolated from horses within England and Ireland. These locations are geographically distinct, but the S. pneumoniae present in these horse populations displayed a tight similarity in a genetic sense.
  • Interestingly, while these horse-based isolates were similar to each other, they were noticeably different from over 120 divergent isolates of S. pneumoniae found in human hosts.

Analysis of Virulence Determinants

  • The researchers undertook a comprehensive exploration of known pneumococcal virulence determinants to help understand the pathogenicity of S. pneumoniae in horses.
  • Surprisingly, the equine isolates seemed to lack activities associated with both pneumolysin and the major autolysin gene lytA.
  • Pneumolysin, a hemolytic cytotoxin, and LytA, a major autolysin gene, are often considered major virulence factors in S. pneumoniae, contributing to its ability to cause disease in its host.

Molecular Studies and Implications

  • The study showed molecular evidence of deleted sections in the coding sequences of both the lytA and ply genes in the equine pneumococci. This supports the phenotypic data observed, where the pathogenic activities related to these genes were absent in the equine isolates.
  • The discoveries made in the study hold significant implications for the understanding of how equine S. pneumoniae evolved and its pathogenicity in horses.
  • The absence of two major virulence factors in the equine isolates of S. pneumoniae changes our understanding of the bacteria’s ability to cause disease, and may have implications for understanding the disease in humans as well.

Cite This Article

APA
Whatmore AM, King SJ, Doherty NC, Sturgeon D, Chanter N, Dowson CG. (1999). Molecular characterization of equine isolates of Streptococcus pneumoniae: natural disruption of genes encoding the virulence factors pneumolysin and autolysin. Infect Immun, 67(6), 2776-2782. https://doi.org/10.1128/IAI.67.6.2776-2782.1999

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 67
Issue: 6
Pages: 2776-2782

Researcher Affiliations

Whatmore, A M
  • Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. a.m.whatwore@warwick.ac.uk
King, S J
    Doherty, N C
      Sturgeon, D
        Chanter, N
          Dowson, C G

            MeSH Terms

            • Animals
            • Artificial Gene Fusion
            • Bacterial Proteins
            • Base Sequence
            • DNA, Bacterial
            • Enzymes / genetics
            • Genes, Bacterial
            • Horse Diseases / microbiology
            • Horses
            • Humans
            • Molecular Sequence Data
            • N-Acetylmuramoyl-L-alanine Amidase / genetics
            • Pneumococcal Infections / microbiology
            • Pneumococcal Infections / veterinary
            • Streptococcus pneumoniae / classification
            • Streptococcus pneumoniae / genetics
            • Streptococcus pneumoniae / isolation & purification
            • Streptococcus pneumoniae / pathogenicity
            • Streptolysins / genetics
            • Virulence

            Grant Funding

            • Wellcome Trust

            References

            This article includes 33 references
            1. Benson CE, Sweeney CR. Isolation of Streptococcus pneumoniae type 3 from equine species.. J Clin Microbiol 1984 Dec;20(6):1028-30.
              pmc: PMC271511pubmed: 6520214doi: 10.1128/jcm.20.6.1028-1030.1984google scholar: lookup
            2. Blunden AS, Mackintosh ME. The microflora of the lower respiratory tract of the horse: an autopsy study.. Br Vet J 1991 May-Jun;147(3):238-50.
              pubmed: 1878768doi: 10.1016/0007-1935(91)90048-rgoogle scholar: lookup
            3. Blunden AS, Hannant D, Livesay G, Mumford JA. Susceptibility of ponies to infection with Streptococcus pneumoniae (capsular type 3).. Equine Vet J 1994 Jan;26(1):22-8.
              pubmed: 8143658doi: 10.1111/j.2042-3306.1994.tb04325.xgoogle scholar: lookup
            4. Burrell MH, Mackintosh ME, Taylor CE. Isolation of Streptococcus pneumoniae from the respiratory tract of horses.. Equine Vet J 1986 May;18(3):183-6.
              pubmed: 3732236doi: 10.1111/j.2042-3306.1986.tb03591.xgoogle scholar: lookup
            5. Chanter N. Streptococcus pneumoniae and equine disease.. Equine Vet J 1994 Jan;26(1):5-6.
              pubmed: 8143665doi: 10.1111/j.2042-3306.1994.tb04319.xgoogle scholar: lookup
            6. Denys GA, Carey RB. Identification of Streptococcus pneumoniae with a DNA probe.. J Clin Microbiol 1992 Oct;30(10):2725-7.
              pmc: PMC270507pubmed: 1400974doi: 10.1128/jcm.30.10.2725-2727.1992google scholar: lookup
            7. Enright MC, Spratt BG. A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease.. Microbiology (Reading) 1998 Nov;144 ( Pt 11):3049-3060.
              pubmed: 9846740doi: 10.1099/00221287-144-11-3049google scholar: lookup
            8. Fenoll A, Martinez-Suarez JV, Muñoz R, Casal J, Garcia JL. Identification of atypical strains of Streptococcus pneumoniae by a specific DNA probe.. Eur J Clin Microbiol Infect Dis 1990 Jun;9(6):396-401.
              pubmed: 2387292doi: 10.1007/bf01979468google scholar: lookup
            9. Flanagan J, Collin N, Timoney J, Mitchell T, Mumford JA, Chanter N. Characterization of the haemolytic activity of Streptococcus equi.. Microb Pathog 1998 Apr;24(4):211-21.
              pubmed: 9533893doi: 10.1006/mpat.1997.0190google scholar: lookup
            10. Hall LM, Whiley RA, Duke B, George RC, Efstratiou A. Genetic relatedness within and between serotypes of Streptococcus pneumoniae from the United Kingdom: analysis of multilocus enzyme electrophoresis, pulsed-field gel electrophoresis, and antimicrobial resistance patterns.. J Clin Microbiol 1996 Apr;34(4):853-9.
              pmc: PMC228905pubmed: 8815096doi: 10.1128/jcm.34.4.853-859.1996google scholar: lookup
            11. Hofer BF, Steck H, Gerber J, Lohrer J, Nicolet J, Paccaud MF. An investigation of the aetiology of viral respiratory disease in a remount depot. Proceedings of the 3rd International Conference on Equine Infectious Diseases 1972; pp. 527–545.
            12. Höltje JV, Tomasz A. Purification of the pneumococcal N-acetylmuramyl-L-alanine amidase to biochemical homogeneity.. J Biol Chem 1976 Jul 25;251(14):4199-207.
              pubmed: 6472
            13. . Sequences. The Institute for Genomic Research 1999.
            14. Kehoe MA. Cell wall associated proteins in Gram-positive bacteria. New Compr Biochem 1994;27:217–261.
            15. Dowson CG, Barcus V, King S, Pickerill P, Whatmore A, Yeo M. Horizontal gene transfer and the evolution of resistance and virulence determinants in Streptococcus.. J Appl Microbiol 1997 Oct;83(S1):42S-51S.
              pubmed: 28621897doi: 10.1046/j.1365-2672.83.s1.5.xgoogle scholar: lookup
            16. Kumar S, Tamura K, Nei M. MEGA: Molecular Evolutionary Genetics Analysis software for microcomputers.. Comput Appl Biosci 1994 Apr;10(2):189-91.
              pubmed: 8019868doi: 10.1093/bioinformatics/10.2.189google scholar: lookup
            17. Mackintosh ME, Grant ST, Burrell MH. Evidence for Streptococcus pneumoniae as a cause of respiratory disease in young thoroughbred horses in training. Equine infectious diseases, no. 5: Proceedings of the Fifth International Conference 1989; pp. 41–44.
            18. Meyer JC, Koterba A, Lester G, Purich BL. Bacteraemia and pneumonia in a neonatal foal caused by Streptococcus pneumoniae type 3.. Equine Vet J 1992 Sep;24(5):407-10.
              pubmed: 1396519doi: 10.1111/j.2042-3306.1992.tb02866.xgoogle scholar: lookup
            19. Mitchell TJ, Andrew PW. Biological properties of pneumolysin.. Microb Drug Resist 1997 Spring;3(1):19-26.
              pubmed: 9109093doi: 10.1089/mdr.1997.3.19google scholar: lookup
            20. Mitchell TJ, Alexander JE, Morgan PJ, Andrew PW. Molecular analysis of virulence factors of Streptococcus pneumoniae.. J Appl Microbiol 1997 Oct;83(S1):62S-71S.
              pubmed: 28621902doi: 10.1046/j.1365-2672.83.s1.7.xgoogle scholar: lookup
            21. Morrison DA. Streptococcal competence for genetic transformation: regulation by peptide pheromones.. Microb Drug Resist 1997 Spring;3(1):27-37.
              pubmed: 9109094doi: 10.1089/mdr.1997.3.27google scholar: lookup
            22. Müller-Graf, C. D. M., A. M. Whatmore, S. J. King, A. P. Pickerill, N. C. Doherty, and C. G. Dowson. Unpublished data.
            23. Neufeld F, Schnitzler R. Pneumokokken Handbuch der Pathogenen Mikro-organismen. 3rd ed. Berlin, Germany: Gustav Fischer; 1928.
            24. Paton JC. The contribution of pneumolysin to the pathogenicity of Streptococcus pneumoniae.. Trends Microbiol 1996 Mar;4(3):103-6.
              pubmed: 8868088doi: 10.1016/0966-842x(96)81526-5google scholar: lookup
            25. Ronda C, García JL, García E, Sánchez-Puelles JM, López R. Biological role of the pneumococcal amidase. Cloning of the lytA gene in Streptococcus pneumoniae.. Eur J Biochem 1987 May 4;164(3):621-4.
              pubmed: 3569279doi: 10.1111/j.1432-1033.1987.tb11172.xgoogle scholar: lookup
            26. Rudolph KM, Parkinson AJ, Black CM, Mayer LW. Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia.. J Clin Microbiol 1993 Oct;31(10):2661-6.
              pmc: PMC265966pubmed: 8253962doi: 10.1128/jcm.31.10.2661-2666.1993google scholar: lookup
            27. Salo P, Ortqvist A, Leinonen M. Diagnosis of bacteremic pneumococcal pneumonia by amplification of pneumolysin gene fragment in serum.. J Infect Dis 1995 Feb;171(2):479-82.
              pubmed: 7844395doi: 10.1093/infdis/171.2.479google scholar: lookup
            28. Selkov E. Metabolic reconstructions. WIT Program, version 2 .
            29. Virolainen A, Salo P, Jero J, Karma P, Eskola J, Leinonen M. Comparison of PCR assay with bacterial culture for detecting Streptococcus pneumoniae in middle ear fluid of children with acute otitis media.. J Clin Microbiol 1994 Nov;32(11):2667-70.
              pmc: PMC264139pubmed: 7852553doi: 10.1128/jcm.32.11.2667-2670.1994google scholar: lookup
            30. Whatmore AM, Barcus VA, Dowson CG. Genetic diversity of the streptococcal competence (com) gene locus.. J Bacteriol 1999 May;181(10):3144-54.
              pmc: PMC93770pubmed: 10322016doi: 10.1128/jb.181.10.3144-3154.1999google scholar: lookup
            31. Wood JL, Burrell MH, Roberts CA, Chanter N, Shaw Y. Streptococci and Pasteurella spp. associated with disease of the equine lower respiratory tract.. Equine Vet J 1993 Jul;25(4):314-8.
              pubmed: 8354218doi: 10.1111/j.2042-3306.1993.tb02970.xgoogle scholar: lookup
            32. Wood JLN, Newton JR, Chanter N, Mumford JA, Townsend HGG, Lakhani KH, Gower SM, Burrell MH, Pilsworth RC, Shepherd M, Hopes R, Dugdale D, Herinckx BMB, Main JPM, Windsor HM, Windsor GD. Longitudinal epidemiological study of respiratory disease in race horses: disease definitions, prevalence, and incidence. Proceedings of the 8th Conference on Equine Infectious Diseases in press.
            33. Yother J, Briles DE. Structural properties and evolutionary relationships of PspA, a surface protein of Streptococcus pneumoniae, as revealed by sequence analysis.. J Bacteriol 1992 Jan;174(2):601-9.
              pmc: PMC205755pubmed: 1729249doi: 10.1128/jb.174.2.601-609.1992google scholar: lookup

            Citations

            This article has been cited 19 times.
            1. Wong ΗE, Tourlomousis P, Paterson GK, Webster S, Bryant CE. Naturally-occurring serotype 3 Streptococcus pneumoniae strains that lack functional pneumolysin and autolysin have attenuated virulence but induce localized protective immune responses.. PLoS One 2023;18(3):e0282843.
              doi: 10.1371/journal.pone.0282843pubmed: 36897919google scholar: lookup
            2. Ginders M, Leschnik M, Künzel F, Kampner D, Mikula C, Steindl G, Eichhorn I, Feßler AT, Schwarz S, Spergser J, Loncaric I. Characterization of Streptococcus pneumoniae isolates from Austrian companion animals and horses.. Acta Vet Scand 2017 Nov 14;59(1):79.
              doi: 10.1186/s13028-017-0348-2pubmed: 29137652google scholar: lookup
            3. Wyllie AL, Pannekoek Y, Bovenkerk S, van Engelsdorp Gastelaars J, Ferwerda B, van de Beek D, Sanders EAM, Trzciński K, van der Ende A. Sequencing of the variable region of rpsB to discriminate between Streptococcus pneumoniae and other streptococcal species.. Open Biol 2017 Sep;7(9).
              doi: 10.1098/rsob.170074pubmed: 28931649google scholar: lookup
            4. Morales M, Martín-Galiano AJ, Domenech M, García E. Insights into the Evolutionary Relationships of LytA Autolysin and Ply Pneumolysin-Like Genes in Streptococcus pneumoniae and Related Streptococci.. Genome Biol Evol 2015 Sep 8;7(9):2747-61.
              doi: 10.1093/gbe/evv178pubmed: 26349755google scholar: lookup
            5. Kilian M, Riley DR, Jensen A, Brüggemann H, Tettelin H. Parallel evolution of Streptococcus pneumoniae and Streptococcus mitis to pathogenic and mutualistic lifestyles.. mBio 2014 Jul 22;5(4):e01490-14.
              doi: 10.1128/mBio.01490-14pubmed: 25053789google scholar: lookup
            6. Park IH, Kim KH, Andrade AL, Briles DE, McDaniel LS, Nahm MH. Nontypeable pneumococci can be divided into multiple cps types, including one type expressing the novel gene pspK.. mBio 2012;3(3).
              doi: 10.1128/mBio.00035-12pubmed: 22532557google scholar: lookup
            7. Denapaite D, Hakenbeck R. A new variant of the capsule 3 cluster occurs in Streptococcus pneumoniae from deceased wild chimpanzees.. PLoS One 2011;6(9):e25119.
              doi: 10.1371/journal.pone.0025119pubmed: 21969869google scholar: lookup
            8. Regev-Yochay G, Hanage WP, Trzcinski K, Rifas-Shiman SL, Lee G, Bessolo A, Huang SS, Pelton SI, McAdam AJ, Finkelstein JA, Lipsitch M, Malley R. Re-emergence of the type 1 pilus among Streptococcus pneumoniae isolates in Massachusetts, USA.. Vaccine 2010 Jul 5;28(30):4842-6.
              doi: 10.1016/j.vaccine.2010.04.042pubmed: 20434550google scholar: lookup
            9. van der Linden M, Al-Lahham A, Nicklas W, Reinert RR. Molecular characterization of pneumococcal isolates from pets and laboratory animals.. PLoS One 2009 Dec 14;4(12):e8286.
              doi: 10.1371/journal.pone.0008286pubmed: 20011527google scholar: lookup
            10. Nahm MH, Lin J, Finkelstein JA, Pelton SI. Increase in the prevalence of the newly discovered pneumococcal serotype 6C in the nasopharynx after introduction of pneumococcal conjugate vaccine.. J Infect Dis 2009 Feb 1;199(3):320-5.
              doi: 10.1086/596064pubmed: 19099489google scholar: lookup
            11. Chi F, Leider M, Leendertz F, Bergmann C, Boesch C, Schenk S, Pauli G, Ellerbrok H, Hakenbeck R. New Streptococcus pneumoniae clones in deceased wild chimpanzees.. J Bacteriol 2007 Aug;189(16):6085-8.
              doi: 10.1128/JB.00468-07pubmed: 17586649google scholar: lookup
            12. Basset A, Trzcinski K, Hermos C, O'Brien KL, Reid R, Santosham M, McAdam AJ, Lipsitch M, Malley R. Association of the pneumococcal pilus with certain capsular serotypes but not with increased virulence.. J Clin Microbiol 2007 Jun;45(6):1684-9.
              doi: 10.1128/JCM.00265-07pubmed: 17392439google scholar: lookup
            13. Llull D, López R, García E. Characteristic signatures of the lytA gene provide a basis for rapid and reliable diagnosis of Streptococcus pneumoniae infections.. J Clin Microbiol 2006 Apr;44(4):1250-6.
              doi: 10.1128/JCM.44.4.1250-1256.2006pubmed: 16597847google scholar: lookup
            14. Wood JL, Newton JR, Chanter N, Mumford JA. Association between respiratory disease and bacterial and viral infections in British racehorses.. J Clin Microbiol 2005 Jan;43(1):120-6.
              doi: 10.1128/JCM.43.1.120-126.2005pubmed: 15634959google scholar: lookup
            15. Arbique JC, Poyart C, Trieu-Cuot P, Quesne G, Carvalho Mda G, Steigerwalt AG, Morey RE, Jackson D, Davidson RJ, Facklam RR. Accuracy of phenotypic and genotypic testing for identification of Streptococcus pneumoniae and description of Streptococcus pseudopneumoniae sp. nov.. J Clin Microbiol 2004 Oct;42(10):4686-96.
              doi: 10.1128/JCM.42.10.4686-4696.2004pubmed: 15472328google scholar: lookup
            16. McAvin JC, Reilly PA, Roudabush RM, Barnes WJ, Salmen A, Jackson GW, Beninga KK, Astorga A, McCleskey FK, Huff WB, Niemeyer D, Lohman KL. Sensitive and specific method for rapid identification of Streptococcus pneumoniae using real-time fluorescence PCR.. J Clin Microbiol 2001 Oct;39(10):3446-51.
              doi: 10.1128/JCM.39.10.3446-3451.2001pubmed: 11574554google scholar: lookup
            17. Whatmore AM, Efstratiou A, Pickerill AP, Broughton K, Woodard G, Sturgeon D, George R, Dowson CG. Genetic relationships between clinical isolates of Streptococcus pneumoniae, Streptococcus oralis, and Streptococcus mitis: characterization of "Atypical" pneumococci and organisms allied to S. mitis harboring S. pneumoniae virulence factor-encoding genes.. Infect Immun 2000 Mar;68(3):1374-82.
              doi: 10.1128/IAI.68.3.1374-1382.2000pubmed: 10678950google scholar: lookup
            18. Radulovic S, Troyer JM, Beier MS, Lau AO, Azad AF. Identification and molecular analysis of the gene encoding Rickettsia typhi hemolysin.. Infect Immun 1999 Nov;67(11):6104-8.
              doi: 10.1128/IAI.67.11.6104-6108.1999pubmed: 10531273google scholar: lookup
            19. Whatmore AM, Dowson CG. The autolysin-encoding gene (lytA) of Streptococcus pneumoniae displays restricted allelic variation despite localized recombination events with genes of pneumococcal bacteriophage encoding cell wall lytic enzymes.. Infect Immun 1999 Sep;67(9):4551-6.
              doi: 10.1128/IAI.67.9.4551-4556.1999pubmed: 10456899google scholar: lookup
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