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Home / Equine Research Bank / Vet Sci / Molecular Detection of Bovine Papillomavirus DNA in the Plac...
Veterinary sciences2019; 6(1); doi: 10.3390/vetsci6010014

Molecular Detection of Bovine Papillomavirus DNA in the Placenta and Blood of Healthy Mares and Respective Foals.

Abstract: Despite the characteristic species specificity of Papillomaviruses (PVs), the bovine papillomavirus (BPV) types 1, 2, and-more rarely-13, can cross-infect equids, where they are involved in the pathogenesis of sarcoid neoplasms. Sarcoids are locally invasive fibroblastic skin tumors that represent the most common skin neoplasms in horses worldwide. The transmission mechanism of BPV is still controversial in horses. Thus far, direct and indirect routes have been implicated, while vertical transmission has been suggested after the detection of viral DNA in the semen of healthy stallions. Testing of the blood and placenta of non-sarcoid baring mares and their respective foals revealed that the equine placenta can harbor BPV DNA, leading us to speculate a possible prenatal vertical DNA transmission in equids.
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Publication Date: 2019-02-06 PubMed ID: 30736349PubMed Central: PMC6466198DOI: 10.3390/vetsci6010014Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research discusses the detection of Bovine Papillomavirus (BPV) DNA in the placenta and blood of healthy horses and their foals. It suggests that the equine placenta can potentially harbor this virus, leading to speculations about prenatal transmission of this virus in horses.

Understanding Papillomaviruses and Their Involvement in Sarcoid Neoplasms

  • Papillomaviruses (PVs), including the Bovine Papillomavirus (BPV), are known for their species specificity. This means they usually infect a specific species and are generally not cross-transmissible to others.
  • However, the research paper indicates the rare instances where BPV types 1, 2, and occasionally 13, can cross-infect horses or equids. This cross-infection is implicated in causing sarcoid neoplasms in horses.
  • Sarcoids are locally invasive fibroblastic skin tumors, known to be the most common form of skin neoplasms in horses worldwide.

Bovine Papillomavirus Transmission Mechanisms

  • The exact mechanism of how BPV is transmitted in horses is still a topic for debate and investigation. Up until now, both direct and indirect ways have been suggested.
  • This research paper introduces the possibility of vertical transmission. This method suggests that the virus could be transmitted from parent to offspring during the gestation period. Evidence supporting this comes from detecting viral DNA in the semen of healthy male horses.

Presence of BPV DNA in the Placenta and Blood

  • In the research, the blood and placenta of healthy mares (female horses) and their foals that didn’t show signs of sarcoid tumors, were tested.
  • The results indicated the presence of BPV DNA in the equine placenta. This finding has led to an important hypothesis arguing for a possible prenatal vertical DNA transmission in horses, meaning the virus could be passed from mother to foal before birth.
  • If proven true, this could lead to radical changes in how BPV infection in horses is understood, leading to new preventative therapies or treatments.

Cite This Article

APA
Savini F, Gallina L, Mazza F, Mariella J, Castagnetti C, Scagliarini A. (2019). Molecular Detection of Bovine Papillomavirus DNA in the Placenta and Blood of Healthy Mares and Respective Foals. Vet Sci, 6(1). https://doi.org/10.3390/vetsci6010014

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 6
Issue: 1

Researcher Affiliations

Savini, Federica
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50 Ozzano Emilia, Bologna 40064, Italy. federica.savini3@unibo.it.
Gallina, Laura
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50 Ozzano Emilia, Bologna 40064, Italy. laura.gallina@unibo.it.
Mazza, Francesca
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50 Ozzano Emilia, Bologna 40064, Italy. francesca.mazza7@unibo.it.
Mariella, Jole
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50 Ozzano Emilia, Bologna 40064, Italy. jole.mariella2@unibo.it.
Castagnetti, Carolina
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50 Ozzano Emilia, Bologna 40064, Italy. carolina.castagnetti@unibo.it.
Scagliarini, Alessandra
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50 Ozzano Emilia, Bologna 40064, Italy. alessand.scagliarini@unibo.it.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 32 references
  1. Chambers G, Ellsmore V.A, O’Brien P.M, Reid S.W.J, Love S, Campo M.S, Nasir L. Association of bovine papillomavirus with the equine sarcoid.. J. Gen. Virol. 2003;84:1055–1062.
    doi: 10.1099/vir.0.18947-0pubmed: 12692268google scholar: lookup
  2. Lunardi M, de Alcântara B.K, Otonel R.A, Rodrigues W.B, Alfieri A.F, Alfieri A.A. Bovine papillomavirus type 13 DNA in equine sarcoids.. J. Clin. Microbiol. 2013;51:2167–2171.
    doi: 10.1128/JCM.00371-13pmc: PMC3697707pubmed: 23637294google scholar: lookup
  3. Van Dyk E, Oosthuizen M.C, Bosman A.M, Nel P.J, Zimmerman D, Venter E.H. Detection of bovine papillomavirus DNA in sarcoid-affected and healthy free-roaming zebra (Equus zebra) populations in South Africa.. J. Virol. Methods. 2009;158:141–151.
    doi: 10.1016/j.jviromet.2009.02.008pubmed: 19428583google scholar: lookup
  4. Bogaert L, Martens A, De Baere C, Gasthuys F. Detection of bovine papillomavirus DNA on the normal skin and in the habitual surroundings of horses with and without equine sarcoids.. Res. Vet. Sci. 2005;79:253–258.
    doi: 10.1016/j.rvsc.2004.12.003pubmed: 16054896google scholar: lookup
  5. Bogaert L, Martens A, Van Poucke M, Ducatelle R, De Cock H, Dewulf J, De Baere C, Peelman L, Gasthuys F. High prevalence of bovine papillomaviral DNA in the normal skin of equine sarcoid-affected and healthy horses.. Vet. Mic. 2008;129:58–68.
    doi: 10.1016/j.vetmic.2007.11.008pubmed: 18093754google scholar: lookup
  6. Brandt S, Haralambus R, Schoster A, Kirnbauer R, Stanek C. Peripheral blood mononuclear cells represent a reservoir of bovine papillomavirus DNA in sarcoid-affected equines.. J. Gen. Virol. 2008;89:1390–1395.
    doi: 10.1099/vir.0.83568-0pubmed: 18474554google scholar: lookup
  7. Finlay M, Yuan Z, Burden F, Trawford A, Morgan I.M, Campo M.S, Nasir L. The detection of Bovine Papillomavirus type 1 DNA in flies.. Virus Res. 2009;144:315–317.
    doi: 10.1016/j.virusres.2009.04.015pubmed: 19409942google scholar: lookup
  8. Gaynor A.M, Zhu K.W, Dela Cruz F.N., Jr., Affolter V.K, Pesavento P.A. Localization of Bovine Papillomavirus Nucleic Acid in Equine Sarcoids.. Vet. Pathol. 2016;53:567–573.
    doi: 10.1177/0300985815594852pubmed: 26215759google scholar: lookup
  9. Silva M.A, Silva K.M, Jesus A.L, Barros L.O, Corteggio A, Altamura G, Borzacchiello G, Freitas A.C. The presence and gene expression of bovine papillomavirus in the peripheral blood and semen of healthy horses.. Transbound. Emerg. Dis. 2014;61:329–333.
    doi: 10.1111/tbed.12036pubmed: 23210736google scholar: lookup
  10. Savini F, Gallina L, Prosperi A, Battilani M, Bettini G, Scagliarini A. E5 nucleotide polymorphisms suggest quasispecies occurrence in BPV-1 sub-clinically infected horses.. Res. Vet. Sci. 2015;102:80–82.
    pubmed: 26412524
  11. Brandt S, Haralambus R, Shafti-Keramat S, Steinborn R, Stanek C, Kirnbauer R. A Subset of Equine Sarcoids Harbours BPV-1 DNA in a Complex with L1 Major Capsid Protein.. Virology. 2008;375:433–441.
    doi: 10.1016/j.virol.2008.02.014pubmed: 18395238google scholar: lookup
  12. Campo M.S, Jarrett W.F.H, O’Neil B.W, Barron R.J. Latent papillomavirus infection in cattle.. Res. Vet. Sci. 1994;56:151–157.
    doi: 10.1016/0034-5288(94)90097-3pubmed: 8191003google scholar: lookup
  13. Roperto S, Comazzi S, Ciusani E, Paolini F, Borzacchiello G, Esposito I, Roperto F. PBMCs are additional sites of productive infection of bovine papillomavirus type 2.. J. Gen. Vir. 2011;92:1787–1794.
    doi: 10.1099/vir.0.031740-0pubmed: 21525209google scholar: lookup
  14. Freitas A.C, Silva M.A.R, Carvalho C.C.R, Birgel E.H, dos Santos J.F, Beçak W, Stocco dos Santos R.C. Papillomavirus DNA detection in non epithelial tissues: A discussion about bovine papillomavirus.. Communicating Current Research and Educational Topics and Trends in Applied Microbiology 2007;Volume 2:697–703.
  15. Lindsey C.L, Almeida M.E, Vicari C.F, Carvalho C, Yaguiu A, Freitas A.C, Beçak W, Stocco R.C. Bovine papillomavirus DNA in milk, blood, urine, semen, and spermatozoa of bovine papillomavirus-infected animals.. Genet. Mol. Res. 2009;8:310–318.
    doi: 10.4238/vol8-1gmr573pubmed: 19291880google scholar: lookup
  16. Yaguiu A, De Carvalho C, de Freitas A.C, Luiz G. Papillomatosis in cattle: In situ detection of bovine papillomavirus DNA sequences in reproductive tissues.. Braz. J. Morphol. Sci. 2006;23:525–529.
  17. De Carvalho C, de Freitas A.C, Brunner O, Bentim Góes L.G, Yaguiu Cavalcante A, Beçak W, Stocco dos Santos R.C. Bovine papillomavirus type 2 in reproductive tract and gamets of slaughtered bovine females.. Braz. J. Microbiol. 2003;34(Suppl. 1):82–84.
    doi: 10.1590/S1517-83822003000500028google scholar: lookup
  18. Silva M.A, Pontes N.E, Da Silva K.M, Guerra M.M, Freitas A.C. Detection of bovine papillomavirus type 2 DNA in commercial frozen semen of bulls (Bos taurus). Anim. Reprod. Sci. 2011;129:146–151.
    doi: 10.1016/j.anireprosci.2011.11.005pubmed: 22172321google scholar: lookup
  19. Carr E.A, Theon A.P, Madewell B.R, Griffey S.M, Hitchcock M.E. Bovine papillomavirus DNA in neoplastic and nonneoplastic tissues obtained from horses with and without sarcoids in the western United States.. Am. J. Vet. Res. 2001;62:741–744.
    doi: 10.2460/ajvr.2001.62.741pubmed: 11341396google scholar: lookup
  20. Martens A, de Moor A, Demeulemeester J, Peelman L. Polymerase chain reaction analysis of the surgical margins of equine sarcoids for bovine papilloma virus DNA.. J. Gen. Virol. 2003;84:1055–1062.
    doi: 10.1053/jvet.2001.25874pubmed: 11555822google scholar: lookup
  21. Armbruster-Moraes E, Ioshimoto L.M, Leão E, Zugaib M. Presence of Human Papillomavirus DNA in Amniotic Fluids of Pregnant Women with Cervical Lesions.. Gynecol. Oncol. 1994;54:152–158.
    doi: 10.1006/gyno.1994.1185pubmed: 8063239google scholar: lookup
  22. Rombaldi R.L, Serafini E.P, Mandelli J, Zimmermann E, Losquiavo K.P. Perinatal transmission of human papilomavirus DNA.. Virol. J. 2009;6:83.
    doi: 10.1186/1743-422X-6-83pmc: PMC2717078pubmed: 19545396google scholar: lookup
  23. Stocco dos Santos R.C, Lindsey C.J, Ferraz O.P, Pinto J.R, Mirandola R.S, Benesi F.J, Birgel E.H, Pereira C.A, Beça W. Bovine papillomavirus transmission and chromosomal aberrations: An experimental model.. J. Gen. Virol. 1998;79:2127–2135.
    doi: 10.1099/0022-1317-79-9-2127pubmed: 9747721google scholar: lookup
  24. Russo V, Paciello O, Zicarelli G, Urraro C, Ceccarelli D.M, De Falco F, Roperto S. Placental papillomatosis in water buffalo associated with bovine deltapapillomavirus.. Proc. LXXI Soc. Vet. Sci. 2017;71:155.
  25. Roperto S, Russo V, Corrado F, Munday J.S, De Falco F, Roperto F. Detection of bovine Deltapapillomavirus DNA in peripheral blood of healthy sheep (Ovis aries). Transbound. Emerg. Dis. 2018;65:758–764.
    doi: 10.1111/tbed.12800pubmed: 29330926google scholar: lookup
  26. Sarkola M.E, Grénman S.E, Rintala M.A, Syrjänen K.J, Syrjänen S.M. Human Papillomavirus in the Placenta and Umbilical Cord Blood.. Acta Obstet. Gynecol. Scand. 2008;87:1181–1188.
    doi: 10.1080/00016340802468308pubmed: 18972230google scholar: lookup
  27. Smith E.M, Parker M.A, Rubenstein L.M, Haugen T.H, Hamsikova E, Turek L.P. Evidence for Vertical Transmission of HPV from Mothers to Infants.. Infect. Dis. Obstet. Gynecol. 2010;7.
    doi: 10.1155/2010/326369pmc: PMC2838362pubmed: 20300545google scholar: lookup
  28. Lee S.M, Park J.S, Norwitz E.R, Koo J.N, Oh I.H, Park J.W, Kim S.M, Kim Y.H, Park C.W, Song Y.S. Risk of Vertical Transmission of Human Papillomavirus throughout Pregnancy: A Prospective Study.. PLoS ONE. 2013;8:e66368.
    doi: 10.1371/journal.pone.0066368pmc: PMC3681772pubmed: 23785495google scholar: lookup
  29. Shen-Gunther J, Wang Y, Lai Z, Poage G.M, Perez L, Huang T.H. Deep sequencing of HPV E6/E7 genes reveals loss of genotypic diversity and gain of clonal dominance in high-grade intraepithelial lesions of the cervix.. BMC Genom. 2017;18:231.
    doi: 10.1186/s12864-017-3612-ypmc: PMC5348809pubmed: 28288568google scholar: lookup
  30. Foresta C, Patassini C, Bertoldo A, Menegazzo M, Francavilla F, Barzon L, Ferlin A. Mechanism of human papillomavirus binding to human spermatozoa and fertilizing ability of infected spermatozoa.. PLoS ONE. 2011;6:e15036.
    doi: 10.1371/journal.pone.0015036pmc: PMC3051064pubmed: 21408100google scholar: lookup
  31. Hermonat P.L, Kechelava S, Lowery C.L, Korourian S. Trophoblasts are the preferential target for human papilloma virus infection in spontaneously aborted products of conception.. Hum. Pathol. 1998;29:170–174.
    doi: 10.1016/S0046-8177(98)90228-3pubmed: 9490277google scholar: lookup
  32. Roperto S, Borzacchiello G, Esposito I, Riccardi M, Urraro C, Lucà R, Corteggio A, Tatè R, Cermola M, Paciello O. Productive infection of bovine papillomavirus type 2 in the placenta of pregnant cows affected with urinary bladder tumors.. PLoS ONE. 2012;7:e33569.
    doi: 10.1371/journal.pone.0033569pmc: PMC3313941pubmed: 22479413google scholar: lookup

Citations

This article has been cited 7 times.
  1. De Falco F, Cutarelli A, Leonardi L, Marcus I, Roperto S. Vertical Intrauterine Bovine and Ovine Papillomavirus Coinfection in Pregnant Cows. Pathogens 2024 May 26;13(6).
    doi: 10.3390/pathogens13060453pubmed: 38921751google scholar: lookup
  2. Pontes NE, Carrazzoni PG, Pessoa-Junior ME, Tibúrcio Júnior E, Freitas AC, Silva MARD. Bovine papillomavirus vertical transmission: BPV diversity and expression in maternal and fetal tissues. Braz J Microbiol 2024 Sep;55(3):2879-2884.
    doi: 10.1007/s42770-024-01394-ypubmed: 38801639google scholar: lookup
  3. Maggi R, De Paolis L, De Santis D, Vellone VG, De Ciucis CG, Fruscione F, Mazzocco K, Ghelardi A, Marruchella G, Razzuoli E. Bovine Papillomavirus Type 1 Infection in an Equine Congenital Papilloma. Pathogens 2023 Aug 18;12(8).
    doi: 10.3390/pathogens12081059pubmed: 37624019google scholar: lookup
  4. Gharban HAJ, Al-Shaeli SJJ, Hussen TJ. Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis. Open Vet J 2023 Jan;13(1):26-41.
    doi: 10.5455/OVJ.2023.v13.i1.4pubmed: 36777440google scholar: lookup
  5. Hassanien RT, Hamdy ME, Elnomrosy SM, Hussein HA, Afify AF, Darwish FM, Shehab G, Emran R, Abd-El-Moniem MII, Habashi AR, Fahmy HA, Ibraheem EM, Shahein MA, Attya M, Abdelhakim AMM, Hagag NM. Molecular characterization and pathological identification of a novel strain of delta papillomavirus-4 (bovine papillomavirus-2) in Egypt. Vet World 2021 Sep;14(9):2296-2305.
    doi: 10.14202/vetworld.2021.2296-2305pubmed: 34840446google scholar: lookup
  6. Savini F, Gallina L, Prosperi A, Puleio R, Lavazza A, Di Marco P, Tumino S, Moreno A, Lelli D, Guercio A, Scagliarini A. Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily. Pathogens 2020 May 30;9(6).
    doi: 10.3390/pathogens9060429pubmed: 32486181google scholar: lookup
  7. Russo V, Roperto F, De Biase D, Cerino P, Urraro C, Munday JS, Roperto S. Bovine Papillomavirus Type 2 Infection Associated with Papillomatosis of the Amniotic Membrane in Water Buffaloes (Bubalus bubalis). Pathogens 2020 Apr 4;9(4).
    doi: 10.3390/pathogens9040262pubmed: 32260380google scholar: lookup
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