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Home / Equine Research Bank / Vet Res Commun / Molecular genotyping of Theileria spp. detected in horses fr...
Veterinary research communications2024; 49(1); 54; doi: 10.1007/s11259-024-10618-3

Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina.

Abstract: After the previous description and characterization of Theileria equi sensu stricto (Genotype A) in Argentina, the aim of the present study was to investigate the possible heterogeneity of 18 S rDNA genotypes circulating in this country. Therefore, blood samples from 18 horses from two different neighborhoods of Corrientes City, Corrientes Province, were analyzed for infection with Theileria species. Theileria DNA was detected in five samples (27.8%). Four nearly complete 18 S rDNA gene sequences were obtained and phylogenetic analyses were carried out. The maximum likelihood tree constructed in this study revealed that two different 18 S rDNA genotypes - namely Genotype A (T. equi sensu stricto) and Genotype C (T. equi sensu lato/Theileria haneyi) - are circulating in the same horse population. These results represent to first evidence of T. equi genotype heterogeneity in Argentina and also the first detection of a member of the T. haneyi/Genotype C group. Together with reports from Brazil, the results of this study reinforce the assumption that Genotypes A and C are the predominant genotypes in South America.
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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Publication Date: 2024-12-24 PubMed ID: 39718660PubMed Central: 7236219DOI: 10.1007/s11259-024-10618-3Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers analyzed the blood samples of horses from Corrientes City, Argentina to identify the different genotypes of the parasite Theileria that may be present. They found that there were two dominant genotypes.

Background of the Study

  • Theileria is a genus of parasites that causes disease in livestock like cows and horses.
  • The main focus of the researchers was Theileria equi, a species of Theileria, which was previously known and characterized in Argentina.
  • Notably, Theileria equi has multiple genotypes, or variations within the species.
  • Prior to this research, only Genotype A (T. equi sensu stricto) had been identified in Argentina, and this study aimed to investigate the possible existence of other genotypes.

Research Method

  • The researchers collected blood samples from 18 horses, resident in two different neighborhoods in Corrientes City.
  • These samples were analyzed to detect the presence of Theileria DNA.
  • From the samples testing positive for Theileria, they extracted nearly complete 18 S rDNA sequences.
  • Phylogenetic analysis, an assessment method to determine the evolutionary relationships between organisms, was conducted on these sequences.

Findings

  • Theileria DNA was detected in five of the 18 samples, implying a 27.8% prevalence rate in the studied horse population.
  • The researchers discovered the presence of not one but two genotypes, namely Genotype A (T. equi sensu stricto) and Genotype C (T. equi sensu lato/Theileria haneyi), within the population studied.
  • This marks two key firsts: the first evidence of T. equi genotype heterogeneity in Argentina and the first-ever detection of a member of the T. haneyi/Genotype C group.

Conclusion

  • The finding that Genotypes A and C are the predominant genotypes in the sampled region in Argentina supports previous assumptions channeled from reports in Brazil.
  • This research reveals a broader range of Theileria genotypes impacting horses in South America than was previously understood.

Cite This Article

APA
Benitez-Ibalo AP, Debárbora VN, Mangold AJ, Nava S, Sebastian PS. (2024). Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina. Vet Res Commun, 49(1), 54. https://doi.org/10.1007/s11259-024-10618-3

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 49
Issue: 1
Pages: 54

Researcher Affiliations

Benitez-Ibalo, Alicia P
  • Laboratorio de Biología de los Parásitos, Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Corrientes, Argentina.
Debárbora, Valeria N
  • Laboratorio de Biología de los Parásitos, Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Corrientes, Argentina.
Mangold, Atilio J
  • Instituto de Investigación de la Cadena Láctea (IDICAL, INTA-CONICET), Estación Experimental Agropecuaria Rafaela, RN N° 34, KM 227, Rafaela, CP 2300, Santa Fe, Argentina.
Nava, Santiago
  • Instituto de Investigación de la Cadena Láctea (IDICAL, INTA-CONICET), Estación Experimental Agropecuaria Rafaela, RN N° 34, KM 227, Rafaela, CP 2300, Santa Fe, Argentina.
Sebastian, Patrick S
  • Instituto de Investigación de la Cadena Láctea (IDICAL, INTA-CONICET), Estación Experimental Agropecuaria Rafaela, RN N° 34, KM 227, Rafaela, CP 2300, Santa Fe, Argentina. sebastian.patrick@inta.gob.ar.

MeSH Terms

  • Animals
  • Theileria / genetics
  • Theileria / classification
  • Theileria / isolation & purification
  • Horses
  • Argentina / epidemiology
  • Horse Diseases / parasitology
  • Horse Diseases / epidemiology
  • Theileriasis / parasitology
  • Theileriasis / epidemiology
  • Genotype
  • Phylogeny
  • RNA, Ribosomal, 18S / genetics
  • DNA, Protozoan / genetics

Grant Funding

  • PI 21F018 / Secretaru00eda General de Ciencia y Tu00e9cnica, Universidad Nacional del Nordeste
  • PI 21F018 / Secretaru00eda General de Ciencia y Tu00e9cnica, Universidad Nacional del Nordeste
  • PICT 2019-970 / Agencia Nacional de Promociu00f3n Cientu00edfica y Tecnolu00f3gica
  • PICT 2019-970 / Agencia Nacional de Promociu00f3n Cientu00edfica y Tecnolu00f3gica
  • PICT 2019-970 / Agencia Nacional de Promociu00f3n Cientu00edfica y Tecnolu00f3gica

Conflict of Interest Statement

Declarations. Ethical approval: The study protocol was approved by the Animal Ethical Commission of the CICUAE-CERSAN of Instituto Nacional de Tecnología Agropecuaria (protocol no. P19-0010). Competing interests: The authors declare no competing interests.

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