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Viruses2019; 11(10); 916; doi: 10.3390/v11100916

Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and Abortion.

Abstract: Equine herpesvirus 1 (EHV-1) is an Alphaherpesvirus infecting not only horses but also other equid and non-equid mammals. It can cause respiratory distress, stillbirth and neonatal death, abortion, and neurological disease. The different forms of disease induced by EHV-1 infection can have dramatic consequences on the equine industry, and thus the virus represents a great challenge for the equine and scientific community. This report describes the progress of a major EHV-1 outbreak that took place in Normandy in 2009, during which the three forms of disease were observed. A collection of EHV-1 strains isolated in France and Belgium from 2012 to 2018 were subsequently genetically analysed in order to characterise EHV-1 strain circulation. The open reading frame 30 (ORF30) non-neuropathogenic associated mutation A was the most represented among 148 samples analysed in this study. ORF30 was also sequenced for 14 strains and compared to previously published sequences. Finally, a more global phylogenetic approach was performed based on a recently described Multilocus Sequence Typing (MLST) method. French and Belgian strains were clustered with known strains isolated in United Kingdom and Ireland, with no correlation between the phylogeny and the time of collection or location. This new MLST approach could be a tool to help understand epidemics in stud farms.
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Publication Date: 2019-10-04 PubMed ID: 31590336PubMed Central: PMC6832873DOI: 10.3390/v11100916Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper investigates the molecular patterns of the Equine herpesvirus 1 (EHV-1) that circulated in France during and after an outbreak in 2009. It also explores how the virus might have evolved and migrated over time by genetically analysing samples taken from 2012 to 2018 in France and Belgium.

Context and Purpose

  • The research focuses on Equine herpesvirus 1 (EHV-1), a type of Alphaherpesvirus. This virus affects horses, other equid mammals, and non-equid animals leading to various diseases such as respiratory infection, abortion, stillbirth/neonatal death, and neurological disorders.
  • Different forms of the disease caused by EHV-1 infection can have significant ramifications for the equine industry. Therefore, there is a strong need for effective monitoring and treatment options to control its spread.
  • The specific interest in this study is to understand the progression and characteristics of EHV-1 during an outbreak in Normandy, France, in 2009, as well as its subsequent spread over a span of six years (2012 to 2018) in France and Belgium.

Procedures and Findings

  • The researchers analysed a collection of EHV-1 strains that were isolated from 2012 to 2018. Genetic analysis was conducted to characterise the circulation of these strains.
  • The emphasis was on the non-neuropathogenic associated mutation A in the open reading frame 30 (ORF30), the most represented mutation among the 148 samples analysed.
  • In addition to this, ORF30 of 14 strains was sequenced and compared with previously published sequences aiding the understanding of the genetic lineage and potential mutative behaviours of the virus.

Analysis Technique and Conclusions

  • A phylogenetic approach based on the Multilocus Sequence Typing (MLST) method was used for a more global approach. This method classifies different strains of the same species based on the sequences of several housekeeping genes.
  • The analysis showed that French and Belgian strains clustered with known strains isolated in the UK and Ireland. It was found that there was no correlation between the phylogeny and the time of collection or location.
  • The researchers concluded that this new MLST approach could be a useful tool to help understand epidemics in stud farms and prepare better for them.

Cite This Article

APA
Sutton G, Garvey M, Cullinane A, Jourdan M, Fortier C, Moreau P, Foursin M, Gryspeerdt A, Maisonnier V, Marcillaud-Pitel C, Legrand L, Paillot R, Pronost S. (2019). Molecular Surveillance of EHV-1 Strains Circulating in France during and after the Major 2009 Outbreak in Normandy Involving Respiratory Infection, Neurological Disorder, and Abortion. Viruses, 11(10), 916. https://doi.org/10.3390/v11100916

Publication

Viruses
ISSN: 1999-4915
NlmUniqueID: 101509722
Country: Switzerland
Language: English
Volume: 11
Issue: 10
PII: 916

Researcher Affiliations

Sutton, Gabrielle
  • LABÉO Frank Duncombe, 14280 Saint-Contest, France. gabrielle.sutton@laboratoire-labeo.fr.
  • NORMANDIE UNIV, UNICAEN, BIOTARGEN, 14000 Caen, France. gabrielle.sutton@laboratoire-labeo.fr.
Garvey, Marie
  • Irish Equine Centre, Johnstown, Naas, County Kildare, W91 RH93, Ireland. MGarvey@irishequinecentre.ie.
Cullinane, Ann
  • Irish Equine Centre, Johnstown, Naas, County Kildare, W91 RH93, Ireland. ACullinane@irishequinecentre.ie.
Jourdan, Marion
  • RESPE, 14280 Saint-Contest, France. technicien@respe.net.
Fortier, Christine
  • LABÉO Frank Duncombe, 14280 Saint-Contest, France. christine.fortier@laboratoire-labeo.fr.
  • NORMANDIE UNIV, UNICAEN, BIOTARGEN, 14000 Caen, France. christine.fortier@laboratoire-labeo.fr.
Moreau, Peggy
  • Clinique équine de la Boisrie, 61500, Chailloué, France. peg_moreau@yahoo.fr.
Foursin, Marc
  • Clinique équine de la Boisrie, 61500, Chailloué, France. clin.eq.laboisrie@laposte.net.
Gryspeerdt, Annick
  • Equi Focus Point Belgium, 8900 Ypres, Belgium. loic.legrand@laboratoire-labeo.fr.
Maisonnier, Virginie
  • RESPE, 14280 Saint-Contest, France. contact@respe.net.
Marcillaud-Pitel, Christel
  • RESPE, 14280 Saint-Contest, France. romain.paillot@laboratoire-labeo.fr.
Legrand, Loïc
  • LABÉO Frank Duncombe, 14280 Saint-Contest, France. stephane.pronost@laboratoire-labeo.fr.
  • NORMANDIE UNIV, UNICAEN, BIOTARGEN, 14000 Caen, France. stephane.pronost@laboratoire-labeo.fr.
Paillot, Romain
  • LABÉO Frank Duncombe, 14280 Saint-Contest, France. c.marcillaud-pitel@respe.net.
  • NORMANDIE UNIV, UNICAEN, BIOTARGEN, 14000 Caen, France. c.marcillaud-pitel@respe.net.
Pronost, Stéphane
  • LABÉO Frank Duncombe, 14280 Saint-Contest, France. annick.gryspeerdt@gmail.com.
  • NORMANDIE UNIV, UNICAEN, BIOTARGEN, 14000 Caen, France. annick.gryspeerdt@gmail.com.

MeSH Terms

  • Abortion, Veterinary / epidemiology
  • Abortion, Veterinary / virology
  • Animals
  • Belgium / epidemiology
  • DNA, Viral / genetics
  • Disease Outbreaks
  • Female
  • France / epidemiology
  • Herpesviridae Infections / epidemiology
  • Herpesvirus 1, Equid / classification
  • Herpesvirus 1, Equid / genetics
  • Herpesvirus 1, Equid / isolation & purification
  • Horse Diseases / epidemiology
  • Horse Diseases / virology
  • Horses
  • Male
  • Multilocus Sequence Typing
  • Nervous System Diseases / epidemiology
  • Open Reading Frames
  • Phylogeny
  • Respiratory Tract Infections / epidemiology
  • Respiratory Tract Infections / veterinary
  • United Kingdom

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 18 times.
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