Myosin isoforms and muscle fiber characteristics in equine gluteus medius muscle.
Abstract: To date, four different myosin heavy chain (MyHC) isoforms have been identified in adult skeletal muscle of a number of species: types I, IIa, IIx or IId, and IIb. The aim of this study was to investigate the distribution of various MyHC isoforms in the equine gluteus medius and gluteus profundus muscles in relation with several morphometric variables of muscle fibers. Methods: Samples from different depths of the gluteus medius muscle (2, 4, 6, and 8 cm) and gluteus profundus muscle of five sedentary horses were examined by MyHC gel electrophoresis, monoclonal antibodies staining against fast, slow and neonatal MyHC isoforms, myosin adenosine triphosphatase (m-ATPase) activity, nicotinamide adenine dinucleotide tetrazolium reductase, alpha-glycerophosphate dehydrogenase, and alpha-amylase-PAS. Data about relative frequencies, sizes, and capillaries of the various histochemical fiber types were collected by morphometry. Results: Three MyHC isoforms were present in the gluteus medius muscle. Two of them comigrated with type I and IIa MyHC isoforms of rat diaphragm (used as a control). The third isoform showed an electrophoretic mobility closer to type IIx than to the IIb MyHC isoform of rat diaphragm. Only two MyHC isoforms (type I and IIa) were detected in the gluteus profundus muscle. In both muscles, type I fibers (high m-ATPase activity at pH 4.5) only reacted with the anti slow-MyHC antibody and both type IIA and IIB fibers (low and moderate m-ATPase activity at pH 4.5, respectively) only reacted with the anti fast-MyHC antibody. No cross-reactivity of fibers positive for both antibodies was found except for the scarce type IIC fibers. Fiber types and capillaries were heterogeneously distributed across the gluteus medius muscle. The deeper regions of this muscle were found to contain a higher percentage of type I fibers, a large number of capillaries and a lower proportion of type IIB fibers compared to the superficial regions of the muscle. The gluteus profundus muscle had more abundant and larger type I fibers than the deepest sampling site of the gluteus medius muscle. Conclusions: These results show the existence of three different MyHC isoforms in the equine gluteus medius muscle and that fiber types and MyHC isoforms are heterogeneously distributed within this muscle. The distribution of slow-twitch and fast-twitch MyHCs among the fibers determined by immunohistochemistry was in agreement with histochemically identified type I and type II fibers, respectively.
Publication Date: 1996-04-01 PubMed ID: 8694280DOI: 10.1002/(SICI)1097-0185(199604)244:4<444::AID-AR3>3.0.CO;2-VGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article examines the distribution of various Myosin Heavy Chain (MyHC) isoforms (types of muscle proteins) in specific muscles of sedentary horses and related them to various characteristics of the muscle fibers.
Research Methodology
- The study involved the examination of samples from different depths of the gluteus medius and profundus muscles from five sedentary horses.
- Different assays were performed on the samples, including MyHC gel electrophoresis, staining using antibodies against various MyHC isoforms, and assessing the activity of myosin adenosine triphosphatase (m-ATPase), among others.
- Data about the frequency, size, and capillaries of the various muscle fiber types were gathered using morphometry.
Research Findings
- Three different MyHC isoforms were found in the gluteus medius muscle, with two of them matching those found in the rat diaphragm (used as a control). The third had a similarity to type IIx.
- Only two MyHC isoforms (type I and IIa) were detected in the gluteus profundus muscle.
- Both muscles had clear distinctions between the fibers with slow and fast MyHCs (type I and II, respectively). Type I fibers had high m-ATPase activity and only reacted with the slow-MyHC antibody, while the Type IIA and IIB fibers (with low/moderate m-ATPase activity) only reacted with the fast-MyHC antibody.
- The distribution of fiber types and capillaries varied across the gluteus medius muscle. The deeper regions contained more type I fibers and capillaries, and fewer type IIB fibers compared to the superficial regions.
- The gluteus profundus muscle contained more abundant and larger type I fibers than the deepest part of the gluteus medius muscle.
Conclusions
- The study concluded that the equine gluteus medius muscle contains three different MyHC isoforms and these, along with the fiber types, had a varied distribution within the muscle.
- The distribution of slow-twitch and fast-twitch MyHCs among the muscle fibers matched the identification of type I and II fibers through histochemistry, confirming the specificity of the antibodies used in the study.
Cite This Article
APA
Serrano AL, Petrie JL, Rivero JL, Hermanson JW.
(1996).
Myosin isoforms and muscle fiber characteristics in equine gluteus medius muscle.
Anat Rec, 244(4), 444-451.
https://doi.org/10.1002/(SICI)1097-0185(199604)244:4<444::AID-AR3>3.0.CO;2-V Publication
Researcher Affiliations
- Department of Comparative Anatomy and Pathological Anatomy, Faculty of Veterinary Sciences, University of Córdoba, Spain.
MeSH Terms
- Adenosine Triphosphatases / analysis
- Analysis of Variance
- Animals
- Buttocks
- Electrophoresis
- Female
- Histocytochemistry
- Horses / metabolism
- Immunohistochemistry
- Isomerism
- Male
- Muscle Fibers, Skeletal / cytology
- Muscle, Skeletal / blood supply
- Muscle, Skeletal / chemistry
- Muscle, Skeletal / cytology
- Myosin Heavy Chains / analysis
- NAD / analysis
Citations
This article has been cited 17 times.- Bal NC, Periasamy M. Uncoupling of sarcoendoplasmic reticulum calcium ATPase pump activity by sarcolipin as the basis for muscle non-shivering thermogenesis.. Philos Trans R Soc Lond B Biol Sci 2020 Mar 2;375(1793):20190135.
- Wagner AL, Urschel KL, Lefta M, Esser KA. Effect of gluteus medius muscle sample collection depth on postprandial mammalian target of rapamycin signaling in mature Thoroughbred mares.. Am J Vet Res 2013 Jun;74(6):910-7.
- Pandorf CE, Jiang W, Qin AX, Bodell PW, Baldwin KM, Haddad F. Regulation of an antisense RNA with the transition of neonatal to IIb myosin heavy chain during postnatal development and hypothyroidism in rat skeletal muscle.. Am J Physiol Regul Integr Comp Physiol 2012 Apr;302(7):R854-67.
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- Serrano AL, Rivero JL. Myosin heavy chain profile of equine gluteus medius muscle following prolonged draught-exercise training and detraining.. J Muscle Res Cell Motil 2000 Apr;21(3):235-45.
- Rivero JL, Serrano AL, Barrey E, Valette JP, Jouglin M. Analysis of myosin heavy chains at the protein level in horse skeletal muscle.. J Muscle Res Cell Motil 1999 Feb;20(2):211-21.
- Linnane L, Serrano AL, Rivero JL. Distribution of fast myosin heavy chain-based muscle fibres in the gluteus medius of untrained horses: mismatch between antigenic and ATPase determinants.. J Anat 1999 Apr;194 ( Pt 3)(Pt 3):363-72.
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