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Home / Equine Research Bank / J Clin Microbiol / Nicoletella semolina gen. nov., sp. nov., a new member of Pa...
Journal of clinical microbiology2004; 42(12); 5542-5548; doi: 10.1128/JCM.42.12.5542-5548.2004

Nicoletella semolina gen. nov., sp. nov., a new member of Pasteurellaceae isolated from horses with airway disease.

Abstract: Gram-negative, nonmotile bacteria that are catalase, oxidase, and urease positive are regularly isolated from the airways of horses with clinical signs of respiratory disease. On the basis of the findings by a polyphasic approach, we propose that these strains be classified as Nicoletella semolina gen. nov, sp. nov., a new member of the family Pasteurellaceae. N. semolina reduces nitrate to nitrite but is otherwise biochemically inert; this includes the lack of an ability to ferment glucose and other sugars. Growth is fastidious, and the isolates have a distinctive colony morphology, with the colonies being dry and waxy and looking like a semolina particle that can be moved around on an agar plate without losing their shape. DNA-DNA hybridization data and multilocus phylogenetic analysis, including 16S rRNA gene (rDNA), rpoB, and infB sequencing, clearly placed N. semolina as a new genus in the family Pasteurellaceae. In all the phylogenetic trees constructed, N. semolina is on a distinct branch displaying approximately 5% 16S rDNA, approximately 16% rpoB, and approximately 20% infB sequence divergence from its nearest relative within the family Pasteurellaceae. High degrees of conservation of the 16S rDNA (99.8%), rpoB (99.6%), and infB (99.7%) sequences exist within the species, indicating that N. semolina isolates not only are phenotypically homogeneous but also are genetically homogeneous. The type strain of N. semolina is CCUG43639(T) (DSM16380(T)).
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Publication Date: 2004-12-08 PubMed ID: 15583279PubMed Central: PMC535277DOI: 10.1128/JCM.42.12.5542-5548.2004Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research presented proposes a new classification, Nicoletella semolina gen. nov, sp. nov., of bacteria commonly found in horses experiencing respiratory issues. Demonstrating unique growth and biochemical behaviors, as well as DNA-DNA hybridization data and multilocus phylogenetic analysis, have led to the inclusion of this organism under the Pasteurellaceae family.

Introduction

  • This study portrays a new genus and species of bacteria, classified as Nicoletella semolina gen. nov, sp. nov., that is frequently isolated from horses with respiratory diseases.
  • The bacteria are Gram-negative, nonmotile and exhibit positive reactions for catalase, oxidase and urease – enzymes that play crucial roles in metabolic processes.

Characteristics of Nicoletella semolina

  • Nicoletella semolina reduces nitrate into nitrites but showcases limited biochemical responsiveness, including its inability to ferment glucose and other sugars. This suggests a distinctive metabolic behavior influencing the pathogenicity and survival of the bacteria.
  • Nicoletella semolina exhibits a unique colony morphology. The bacterial colonies bear a dry, waxy appearance, resembling semolina particles. They can be moved on an agar plate while maintaining their form, indicating a robust physical structure.

Genetic Analysis

  • A comprehensive genetic assessment was carried out to better understand Nicoletella semolina’s classification within the Pasteurellaceae family. DNA from different colonies was hybridized and compared. Divergence rates between the Nicoletella semolina and its nearest relative within Pasteurellaceae were around 5% for 16S rDNA, 16% for rpoB, and 20% for infB.
  • Interestingly, high conservation rates were found among the 16S rDNA (99.8%), rpoB (99.6%), and infB (99.7%) sequences within the same species. This indicates that although Nicoletella semolina shows divergence from its nearest Pasteurellaceae relative, it has a high degree of genetic homogeneity within its species.

Conclusion

  • The unique characteristics, both phenotypically and genotypically, led the researchers to propose Nicoletella semolina as a new genus in the family Pasteurellaceae. This might bring new pathways for studying respiratory issues in horses and further understanding of this bacteria’s role in the pathology.
  • The type strain of Nicoletella semolina, acting as a reference point for future studies, is identified as CCUG43639(T) (DSM16380(T)).

Cite This Article

APA
Kuhnert P, Korczak B, Falsen E, Straub R, Hoops A, Boerlin P, Frey J, Mutters R. (2004). Nicoletella semolina gen. nov., sp. nov., a new member of Pasteurellaceae isolated from horses with airway disease. J Clin Microbiol, 42(12), 5542-5548. https://doi.org/10.1128/JCM.42.12.5542-5548.2004

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 42
Issue: 12
Pages: 5542-5548

Researcher Affiliations

Kuhnert, Peter
  • Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland. peter.kuhnert@vbi.unibe.ch
Korczak, Bozena
    Falsen, Enevold
      Straub, Reto
        Hoops, Anneliese
          Boerlin, Patrick
            Frey, Joachim
              Mutters, Reinier

                MeSH Terms

                • Animals
                • Bacterial Proteins / genetics
                • DNA, Ribosomal / analysis
                • Horse Diseases / microbiology
                • Horses
                • Lung Diseases / microbiology
                • Lung Diseases / veterinary
                • Molecular Sequence Data
                • Nucleic Acid Hybridization
                • Pasteurellaceae / classification
                • Pasteurellaceae / genetics
                • Pasteurellaceae / isolation & purification
                • Pasteurellaceae / metabolism
                • Pasteurellaceae Infections / microbiology
                • Pasteurellaceae Infections / veterinary
                • Phylogeny
                • RNA, Ribosomal, 16S / genetics
                • Sequence Analysis, DNA

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                Citations

                This article has been cited 8 times.
                1. Bond S, McMullen C, Timsit E, Léguillette R. Topography of the respiratory, oral, and guttural pouch bacterial and fungal microbiotas in horses.. J Vet Intern Med 2023 Jan;37(1):349-360.
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                3. Zhu Y, Chen S, Yi Z, Holyoak R, Wang T, Ding Z, Li J. Nasopharyngeal Microbiomes in Donkeys Shedding Streptococcus equi Subspecies equi in Comparison to Healthy Donkeys.. Front Vet Sci 2021;8:645627.
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