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Home / Equine Research Bank / Int J Mol Sci / Novel localization of peripherin 2, the photoreceptor-specif...
International journal of molecular sciences2015; 16(2); 2678-2692; doi: 10.3390/ijms16022678

Novel localization of peripherin 2, the photoreceptor-specific retinal degeneration slow protein, in retinal pigment epithelium.

Abstract: Retinal pigment epithelium (RPE) builds the outer blood-retinal barrier of the eye. Since one typical feature of the autoimmune disease, equine recurrent uveitis (ERU), is the breakdown of this barrier, we recently performed comparative analysis of healthy and uveitic RPE. We identified for the first time peripherin 2, which is responsible for visual perception and retina development, to be localized in RPE. The purpose of this study was therefore to validate our findings by characterizing the expression patterns of peripherin 2 in RPE and retina. We also investigated whether peripherin 2 expression changes in ERU and if it is expressed by the RPE itself. Via immunohistochemistry, significant downregulation of peripherin 2 in uveitic RPE compared to the control was detectable, but there was no difference in healthy and uveitic retina. A further interesting finding was the clear distinction between peripherin 2 and the phagocytosis marker, rhodopsin, in healthy RPE. In conclusion, changes in the expression pattern of peripherin 2 selectively affect RPE, but not retina, in ERU. Moreover, peripherin 2 is clearly detectable in healthy RPE due to both phagocytosis and the expression by the RPE cells themselves. Our novel findings are very promising for better understanding the molecular mechanisms taking place on RPE in uveitis.
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Publication Date: 2015-01-26 PubMed ID: 25629227PubMed Central: PMC4346858DOI: 10.3390/ijms16022678Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study identifies for the first time the presence of peripherin 2 protein, known for its role in visual perception and retina development, in retinal pigment epithelium (RPE). The study investigates the changes in peripherin 2 expression in conditions of autoimmune disease, namely equine recurrent uveitis (ERU), which is characterized by a breakdown of the RPE barrier.

Objective of Research

  • The main purpose of the research was to validate the previously identified presence of peripherin 2 in the retinal pigment epithelium (RPE), a layer that helps form the outer blood-retinal barrier in the eye. The study aims to characterize the expression patterns of this protein in both RPE and the retina.

Methodology Explained

  • The research utilized immunohistochemistry, a process that involves the use of antibodies to detect the presence and distribution of specific proteins in tissue samples. This technique enabled the researchers to observe the expression level of peripherin 2 in both healthy and uveitic (inflamed) RPE and retina.

Major Findings

  • The study found that in cases of equine recurrent uveitis (ERU), there was significant downregulation of peripherin 2 in the RPE as compared to the control. However, no difference was noted in the retina, healthy or affected by ERU.
  • Another noteworthy observation was the distinction between the localization of peripherin 2 and rhodopsin, a marker for phagocytosis, in the healthy RPE. This suggested that peripherin 2 in RPE is detectable due to both the process of phagocytosis and direct expression by the RPE cells.

Conclusions and Implications

  • The results of this study suggest that changes in the expression pattern of peripherin 2 selectively affects the RPE but not the retina in the case of ERU. Hence, the newly discovered presence of peripherin 2 in RPE may shed light on the molecular mechanisms taking place in uveitis conditions.
  • The findings can potentially unlock new avenues for better understanding the pathogenesis of retinal diseases and could be a step forward in developing novel therapeutic measures for uveitis.

Cite This Article

APA
Uhl PB, Amann B, Hauck SM, Deeg CA. (2015). Novel localization of peripherin 2, the photoreceptor-specific retinal degeneration slow protein, in retinal pigment epithelium. Int J Mol Sci, 16(2), 2678-2692. https://doi.org/10.3390/ijms16022678

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 16
Issue: 2
Pages: 2678-2692

Researcher Affiliations

Uhl, Patrizia B
  • Institute for Animal Physiology, Department of Veterinary Sciences, Ludwig-Maximilians-University, Veterinärstraße 13, D-80539 Munich, Germany. p.uhl@tiph.vetmed.uni-muenchen.de.
Amann, Barbara
  • Institute for Animal Physiology, Department of Veterinary Sciences, Ludwig-Maximilians-University, Veterinärstraße 13, D-80539 Munich, Germany. baerbl.amann@tiph.vetmed.uni-muenchen.de.
Hauck, Stefanie M
  • Research Unit for Protein Science, Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany. hauck@helmholtz-muenchen.de.
Deeg, Cornelia A
  • Institute for Animal Physiology, Department of Veterinary Sciences, Ludwig-Maximilians-University, Veterinärstraße 13, D-80539 Munich, Germany. deeg@tiph.vetmed.uni-muenchen.de.

MeSH Terms

  • Animals
  • Cells, Cultured
  • Down-Regulation
  • Horses
  • Humans
  • Immunohistochemistry
  • Peripherins / metabolism
  • Phagocytosis
  • Retinal Pigment Epithelium / cytology
  • Retinal Pigment Epithelium / metabolism
  • Rhodopsin / metabolism
  • Uveitis / metabolism
  • Uveitis / pathology
  • Uveitis / veterinary

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