Oligonucleotide probes for DNA fingerprinting in horses.
Abstract: 10 different oligonucleotide probes were evaluated for DNA fingerprinting in horses. Five probes were able to detect polymorphic bands. The probes (GT)(8) , (GTG)(5) and (GGAT)(4) are most informative for individual identification and were used to analyze a population of Hannoveranian horses. The probability that two individuals have the same DNA fingerprint pattern is 1.2 × 10(-8) , 5.2 × 10(-10) and 1.5 × 10(-7) respectively. Using a combination of the three probes, paternity tests were performed with exclusion probabilities between 0.08% and 4%. ZUSAMMENFASSUNG: Oligonukleotide-Sonden für DNS-Fingerprints von Pferden Zur Darstellung von DNA-Fingerprints beim Pferd wurden zehn verschiedene Oligonukleotid-Sonden verglichen. Mit fünf Sonden konnten polymorphe Banden nachgewiesen werden. Die Sonden (GT)(8) , (GTG)(5) und (GGAT)(4) besaßen die größte Informativität für den Identitätsnachweis und wurden für die Analyse einer Population von Hannoverschen Pferden benutzt. Die Wahrscheinlichkeit, daß zwei Individuen dieselben Fingerprint-Muster aufweisen, liegt bei 1,2 × 10(-8) , 5,2 × 10(-10) bzw. 1,5 × 10(-7) . Bei Verwendung einer Kombination der drei Sonden wurden Vaterschaftskontrollen mit Ausschlußwahrscheinlichkeiten zwischen 0,08% und 4% erreicht.
1993 Blackwell Verlag GmbH.
Publication Date: 1993-01-12 PubMed ID: 21395728DOI: 10.1111/j.1439-0388.1993.tb00741.xGoogle Scholar: Lookup
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Summary
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This research focuses on the application of oligonucleotide probes for DNA fingerprinting in horses, evaluating ten different probes and confirming the effectiveness of three probes, namely (GT)(8), (GTG)(5) and (GGAT)(4) in identifying individual horses with a very low probability of duplication.
Probing DNA Fingerprinting in Horses
- The study began by assessing ten different oligonucleotide probes. These probes are short sequences of DNA used to detect the presence of a complementary DNA sequence in a sample. Here, they were used for the purpose of DNA fingerprinting, a method used to identify individuals based on their unique DNA profiles.
- Out of the ten probes, five were effective at detecting polymorphic bands. A polymorphic band is a term used to describe a DNA fragment that varies in size and appears at a specific location. This variability can be used to distinguish between different individuals.
Identifying the Most Informative Probes
- The probes (GT)(8), (GTG)(5) and (GGAT)(4) were found to be most informative for the identification of individuals. This means that they produced the widest range of variations, which makes them valuable for individual identification.
- These three probes were then used to analyze a population of Hannoveranian horses. The Hannoveranian horse is a warmblood breed originating from Germany, known for their strength, agility, and adaptability.
Probing Results and Paternity Tests
- The research found that the probability of two horses having the same DNA fingerprint pattern using probes (GT)(8), (GTG)(5) and (GGAT)(4) was respectively 1.2 × 10(-8), 5.2 × 10(-10) and 1.5 × 10(-7). This very low probability confirms the high specificity of these probes, meaning they can effectively discriminate between individual horses.
- In addition to individual identification, the combination of these three probes was used to perform paternity tests. The results showed exclusion probabilities varying between 0.08% and 4%. This means that when these probes were used, the error margin in determining the correct father varied between 0.08% and 4%, signifying a high degree of accuracy.
Cite This Article
APA
Wilke K, Weimann M, Jung M, Geldermann H.
(1993).
Oligonucleotide probes for DNA fingerprinting in horses.
J Anim Breed Genet, 110(1-6), 301-304.
https://doi.org/10.1111/j.1439-0388.1993.tb00741.x Publication
Researcher Affiliations
- Institut für Tierhaltung und Tierzüchtung, Fachgebiet Tierzüchtung, Universität Hohenheim, Stuttgart, Germany.
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