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Equine veterinary journal2010; 43(3); 288-294; doi: 10.1111/j.2042-3306.2010.00215.x

Optimisation of bone marrow aspiration from the equine sternum for the safe recovery of mesenchymal stem cells.

Abstract: Mesenchymal stem cell (MSC) therapy for orthopaedic disease is being used with increasing frequency; there is a need to define a safe, reliable and effective technique for the recovery of MSCs from the sternum of the horse. Objective: To describe an optimised safe technique for obtaining bone marrow-derived MSCs from the sternum of the Thoroughbred horse. Methods: The anatomical relationship of the sternum with the heart and internal anatomy was demonstrated in cadavers. Sternal anatomy was evaluated ultrasonographically and after midline sectioning. Sternebrae were examined histologically after aspiration to determine the effect of needle insertion. The quality of the aspirate was evaluated as the number of colony-forming units from sequential and separately aspirated 5 ml aliquots and assessed for their multipotency using trilineage differentiation. Results: The optimal safe location for the needle was the 5th sternebra because it had a safe dorsoventral thickness and was cranial to the apex of the heart. This sternebra could be reliably identified ultrasonographically. Aspirates could also be obtained from the 4th and 6th sternebrae, although the former is between the front limbs and the latter closer to the heart. Minimal disruption of the internal bony architecture was seen after needle insertion through the thin outer cortex and the first 5 ml aliquot contained the greatest number of colony-forming units of mesenchymal stem cells with trilineage capabilities. Conclusions: Accurate placement of a Jamshidi needle into the medullary cavity of the 4th-6th individual sternebrae is facilitated by the use of ultrasonography and enables aspiration of bone marrow reliably with minimal damage to the sternum and risk to the horse. Conclusions: Sternal marrow aspiration as described is a safe and reliable technique to obtain MSCs for orthopaedic cell-based therapies.
© 2010 EVJ Ltd.
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Publication Date: 2010-09-23 PubMed ID: 21492205DOI: 10.1111/j.2042-3306.2010.00215.xGoogle Scholar: Lookup
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  • Journal Article

Summary

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The research article introduces a safe and effective technique for retrieving mesenchymal stem cells from a horse’s sternum for treatment of orthopedic diseases.

Objective and Methodology

  • The researchers aimed to outline a safe and optimized technique for retrieving mesenchymal stem cells (MSCs) from the sternum of a Thoroughbred horse. This research became necessary due to the increasing use of MSC therapy for orthopedic diseases in horses.
  • The team investigated the anatomical relationship of the horse’s sternum (breastbone) with its heart and interior anatomy using cadavers. The anatomy of the sternum was further evaluated through ultrasound and midline sectioning.
  • The sternebrae (bone segments of the sternum) were examined histologically after aspiration, a procedure to penetrate the sternebrae to obtain bone cells.
  • The quality of the aspirate (retrieved cells) was measured by the number of colony-forming units from sequential and separately aspirated 5 ml aliquots. These units were also assessed for their trilineage differentiation ability, a key characteristic of MSCs.

Results

  • The optimal safe position for the needle, identified as the 5th sternebra, was determined because of its suitable dorsoventral thickness and its location above the horse’s heart apex. This sternebra could be precisely identified through ultrasonography.
  • Aspirates were also obtainable from the 4th and 6th sternebrae, although the former is between the front limbs and the latter is closer to the heart. Needle insertion through the thin outer cortex resulted in minimal disruption of the internal bony remains.
  • It was discovered that the first 5 ml aliquot had the most substantial number of colony-forming units of MSCs with trilineage capabilities.

Conclusions

  • The research concluded that the correct placement of a Jamshidi needle (used for bone marrow biopsy) into the bone marrow cavity of the 4th-6th individual sternebrae, facilitated by ultrasonography, enables the safe and reliable aspiration of bone marrow.
  • Minimal damage to the horse’s sternum and risk to the horse were also ensured by the optimized technique, thus making sternum marrow aspiration a safe and reliable method to obtain MSCs for orthopedic cell-based therapy applications.

Cite This Article

APA
Kasashima Y, Ueno T, Tomita A, Goodship AE, Smith RK. (2010). Optimisation of bone marrow aspiration from the equine sternum for the safe recovery of mesenchymal stem cells. Equine Vet J, 43(3), 288-294. https://doi.org/10.1111/j.2042-3306.2010.00215.x

Publication

Equine veterinary journal
ISSN: 0425-1644
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 43
Issue: 3
Pages: 288-294

Researcher Affiliations

Kasashima, Y
  • Clinical Science and Pathobiology Division, Equine Research Institute, Japan Racing Association, Tokami-cho, Japan. kasashima@center.equinst.go.jp
Ueno, T
    Tomita, A
      Goodship, A E
        Smith, R K W

          MeSH Terms

          • Animals
          • Biopsy, Needle / veterinary
          • Bone Marrow Cells / physiology
          • Female
          • Horses / physiology
          • Male
          • Mesenchymal Stem Cells / physiology
          • Sternum / physiology

          Citations

          This article has been cited 25 times.
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