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Home / Equine Research Bank / J Vet Sci / Parentage testing of Thoroughbred horse in Korea using micro...
Journal of veterinary science2006; 7(1); 63-67; doi: 10.4142/jvs.2006.7.1.63

Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing.

Abstract: The present study was to construct a parentage testing system for Thoroughbred (TB) horse. A total number of 1,285 TB horse samples including 962 foals for parentage testing, 9 sires and 314 dams for individual identification were genotyped. Genomic DNA was extracted from 5 hair roots and genotyped by using 14 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3 and VHL20). This method consisted of multiplexing PCR procedure and showed reasonable amplification of all PCR products. Genotypes were determined by genetic analyzer. The number of alleles per locus varied from 3 to 9 with a mean value of 6.36 in TB horse. The expected heterozygosity was ranged from 0.548 to 0.831 (mean 0.699), and the total exclusion probability of 14 microstellite loci was 0.9998. Of the 14 markers, ASB2, ASB17, ASB23, HMS7 and HTG10 loci have relatively high PIC value (> 0.7). Of the 962 foals, 960 foals were qualified by compatibility according to the Mendelism. These results suggest that the DNA typing method has high potential for parentage verification and individual identification of TB horses.
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Publication Date: 2006-01-26 PubMed ID: 16434852PubMed Central: PMC3242088DOI: 10.4142/jvs.2006.7.1.63Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study developed a method for determining the parentage of Thoroughbred horses in Korea using microsatellite DNA typing. Of the 962 foal samples tested, the new method was able to accurately identify the parent horses of 960 foals.

Methodology

  • The researchers collected hair root samples from 1,285 Thoroughbred horses, including 962 foals, and genotyped the DNA extracted from the samples.
  • They identified the horse’s genetic structure using 14 microsatellite markers. These are specific DNA sequences that have repeating sections of two to five base pairs. The number of repetitions determines the genetic marker’s length or size, which varies between individuals and can be used for identification and parentage testing.
  • The researchers set up a multiple PCR (Polymerase Chain Reaction) procedure, a method used to amplify segments of DNA. All PCR products showed reasonable amplification.

Findings

  • Genetic analyzer was used to determine the genotypes. It showed that the number of alleles (different forms of the same gene) per locus (a fixed location on a chromosome) ranged from 3 to 9 horses, with an average of 6.36.
  • They measured the expected heterozygosity (genetic diversity within a breed), which ranged from 0.548 to 0.831, with an average of 0.699. Higher levels of heterozygosity indicate more genetic diversity, suggesting a healthy population.
  • The total exclusion probability of the 14 microsatellite loci was 0.9998, almost close to one, implying that the system developed was highly accurate in determining parentage.
  • Of the 962 foals tested for parentage, 960 were qualified, meaning their genetic makeup was consistent with the purported parent horses.

Conclusion

  • The study demonstrated the use of microsatellite DNA typing in verifying parentage and individual identification of Thoroughbred horses. This method could enhance the accuracy of breeding records, which in turn might aid in preserving the breed’s genetic diversity and improving selection for better performing racehorses.

Cite This Article

APA
Lee SY, Cho GJ. (2006). Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing. J Vet Sci, 7(1), 63-67. https://doi.org/10.4142/jvs.2006.7.1.63

Publication

Journal of veterinary science
ISSN: 1229-845X
NlmUniqueID: 100964185
Country: Korea (South)
Language: English
Volume: 7
Issue: 1
Pages: 63-67

Researcher Affiliations

Lee, Sun-young
  • Laboratory of Equine Genetics, Korea Racing Association, Gwacheon 427-711, Korea.
Cho, Gil-jae

    MeSH Terms

    • Alleles
    • Animals
    • DNA / chemistry
    • DNA / genetics
    • DNA Fingerprinting / methods
    • DNA Fingerprinting / veterinary
    • Female
    • Genotype
    • Horses / genetics
    • Korea
    • Male
    • Microsatellite Repeats / genetics
    • Pedigree
    • Polymerase Chain Reaction / veterinary
    • Polymorphism, Genetic

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    Citations

    This article has been cited 7 times.
    1. An J, Tseveen K, Oyungerel B, Kong HS. Analysis of genetic diversity and structure of Mongolian horse using microsatellite markers. J Anim Sci Technol 2022 Nov;64(6):1226-1236.
      doi: 10.5187/jast.2022.e82pubmed: 36812018google scholar: lookup
    2. Park CS, Lee SY, Cho GJ. Evaluation of recent changes in genetic variability in Thoroughbred horses based on microsatellite markers parentage panel in Korea. Anim Biosci 2022 Apr;35(4):527-532.
      doi: 10.5713/ab.21.0272pubmed: 34530514google scholar: lookup
    3. Jaworska J, Ropka-Molik K, Wocławek-Potocka I, Siemieniuch M. Inter- and intrabreed diversity of the major histocompatibility complex (MHC) in primitive and draft horse breeds. PLoS One 2020;15(2):e0228658.
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    4. Kathiravan P, Kataria RS, Mishra BP. Power of exclusion of 19 microsatellite markers for parentage testing in river buffalo (Bubalus bubalis). Mol Biol Rep 2012 Aug;39(8):8217-23.
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    5. Machado FB, de Vasconcellos Machado L, Bydlowski CR, Bydlowski SP, Medina-Acosta E. Gametic phase disequilibrium between the syntenic multiallelic HTG4 and HMS3 markers widely used for parentage testing in Thoroughbred horses. Mol Biol Rep 2012 Feb;39(2):1447-52.
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    6. Stejskalova K, Vychodilova L, Janova E, Oppelt J, Horin P. Innate Immunity Toll-Like Triad TLR6-1-10 and Its Diversity in Distinct Horse Breeds. Vet Med Sci 2025 Mar;11(2):e70230.
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    7. Seo JH, Park KD, Lee HK, Kong HS. Genetic diversity of Halla horses using microsatellite markers. J Anim Sci Technol 2016;58:40.
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