PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum.
Abstract: A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those measured by a time-resolved fluoro-immunoassay (FIA). To monitor physiological changes, serum estradiol concentrations in mares were measured using PATHFAST throughout the gestation period. The serum estradiol concentrations correlated highly with those in whole blood samples. The serum concentrations of estradiol measured by PATHFAST also correlated well with FIA. Circulating estradiol increased during mid-gestation and high levels of serum estradiol were maintained in late gestation, followed by an abrupt decline to term. These results demonstrate the utility of PATHFAST in equine clinics as an accurate diagnostic tool for the rapid assay of estradiol within 26 min using unextracted whole blood.
Publication Date: 2016-08-20 PubMed ID: 27545960PubMed Central: PMC5177982DOI: 10.1262/jrd.2016-038Google Scholar: Lookup
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- Journal Article
Summary
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The study evaluates the effectiveness of PATHFAST, a new chemiluminescent enzyme immunoassay system used to measure the level of estradiol in horses’ blood and serum, comparing it with existing methods.
The PATHFAST System
- The PATHFAST system is a novel chemiluminescent enzyme immunoassay. This type of test is often used in medical and research labs to measure the concentration of compounds in biological fluids.
- In this study, PATHFAST was utilised to measure the concentration of estradiol in horse blood and serum. Estradiol is a form of estrogen, which is a critical hormone in horse reproduction.
- One of PATHFAST’s major advantages is that it can examine unextracted whole blood, which greatly speeds up the diagnostic process.
Comparison with Time-Resolved Fluoro-Immunoassay
- The researchers compared the estradiol concentrations measured by PATHFAST to those measured by a time-resolved fluoro-immunoassay (FIA), a previously established method of hormone assay.
- The results showed that the serum concentrations of estradiol measured by PATHFAST corresponded well with those produced by FIA, suggesting that PATHFAST is an equally accurate measurement tool.
Monitoring Estradiol levels throughout Gestation
- Using PATHFAST, the researchers also tracked changes in serum estradiol concentrations in mares during their gestation period. They observed that estradiol levels rose during mid-gestation and remained high until late gestation, after which they rapidly dropped close to term.
- This pattern of hormonal change can be crucial in monitoring a mare’s pregnancy progression and determining her health status.
Conclusion
- The study demonstrated the effectiveness of using PATHFAST as an accurate diagnostic tool for estradiol assessment, and its potential application in equine clinics.
- This system offers quick results, reportedly within 26 minutes, and can use unextracted whole blood, which reduces the complexity and duration of the sample preparation process.
Cite This Article
APA
Toishi Y, Tsunoda N, Kume K, Nagaoka K, Watanabe G, Taya K.
(2016).
PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum.
J Reprod Dev, 62(6), 631-634.
https://doi.org/10.1262/jrd.2016-038 Publication
Researcher Affiliations
- Shadai Corporation, Hokkaido 059-1432, Japan.
MeSH Terms
- Animals
- Estradiol / blood
- Horses / blood
- Immunoenzyme Techniques / methods
- Immunoenzyme Techniques / veterinary
- Luminescent Measurements / methods
- Luminescent Measurements / veterinary
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Citations
This article has been cited 1 times.- Satoh M, Higuchi T, Inoue S, Gotoh T, Murase H, Nambo Y. Factors affecting the prognosis for uterine torsion: the effect of treatment based on measurements of serum progesterone and estradiol concentrations after surgery. J Equine Sci 2017;28(4):163-167.
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