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Home / Equine Research Bank / PLoS One / The effects of a synthetic epidermis spray on secondary inte...
PloS one2024; 19(3); e0299990; doi: 10.1371/journal.pone.0299990

The effects of a synthetic epidermis spray on secondary intention wound healing in adult horses.

Abstract: To evaluate secondary intention wound healing in the horse's limbs when treated with the synthetic epidermis spray (Novacika®, Cohesive S.A.S, France) or with a standard bandaging technique. Methods: Six Standardbred mares were included in the study. Four 2.5 x 2.5 cm full-thickness skin wounds were created on each thoracic limb. Two wounds were located on the dorsoproximal aspect of the cannon bone and the other two at the dorsoproximal aspect of the fetlock. Six hours after creation, wounds were randomly treated with synthetic epidermis spray or standard bandaging. The wounds were assessed every 4 days by gross visual assessment and using a 3D imaging camera. Analysis was performed with a 3D imaging application. Results: Out of 46 wounds, 22 showed exuberant granulation tissue and were part of the standard bandaging group. Whether the wounds were treated with synthetic epidermis spray or standard bandaging, the time for healing was the same. Conclusions: The synthetic epidermis spray studied in this model has allowed healing without the production of exuberant granulation tissue but did not reduce the median wound healing time compared to a standard bandaging technique. The synthetic epidermis spray is potentially an interesting alternative for the management of secondary intention wound healing of superficial and non-infected distal limb wounds in adult horses on economical and practical aspects. However, all statistical inference (p-values especially) must be interpreted with caution, given the size of the sample.
Copyright: © 2024 Charlotte C. et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Publication Date: 2024-03-07 PubMed ID: 38451976PubMed Central: PMC10919598DOI: 10.1371/journal.pone.0299990Google Scholar: Lookup
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  • Journal Article
  • Randomized Controlled Trial
  • Veterinary

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article focuses on comparing the effectiveness of three diagnostic techniques – the Mini-FLOTAC, Cornell-Wisconsin, and Proudman and Edwards – in identifying and quantifying A. perfoliata, a common tapeworm species in horses. The findings suggest that the Proudman and Edwards technique was the most effective, detecting higher numbers of tapeworm eggs compared to the other two techniques.

Study Design

  • The research was conducted on eighteen adult female horses from the University of Kentucky’s historic parasitology herd.
  • The study had five collection time points, spaced two weeks apart, where fecal samples were taken from all horses and analyzed using the three diagnostic techniques.
  • The study’s design resulted in 90 samples being taken and a total of 270 counts determined, which allowed the researchers to compare the performance of the three methods.

Findings

  • The three diagnostic techniques produced significantly different results in terms of positive sample proportions. The Mini-FLOTAC technique produced a 16% positive rate, the Cornell-Wisconsin had a 47% positive rate, and the Proudman and Edwards method had a 70% positive rate.
  • Moreover, the Proudman and Edwards technique consistently counted higher numbers of tapeworm eggs compared to the other two techniques throughout the study. The total raw counts of tapeworm eggs across the study for each technique were 16 for the Mini-FLOTAC, 88 for the Cornell-Wisconsin, and 410 for the Proudman and Edwards.
  • The study concluded that the Proudman and Edwards technique was superior in diagnosing A. perfoliata infection.

Implications

  • The results of this study had significant implications for the diagnosis and treatment of tapeworm infections in horses. Given the rising concerns over anthelmintic resistance in A. perfoliata, having effective diagnostic techniques is critical.
  • However, the researchers also highlighted the need for future work to assess the potential of the Proudman and Edwards technique for Fecal Egg Count Reduction Testing (FECRT). FECRT can be valuable in detecting anthelmintic resistance, hence playing a crucial role in managing tapeworm infections in horses.

Cite This Article

APA
Charlotte C P, Benoit B, Olivier M L. (2024). The effects of a synthetic epidermis spray on secondary intention wound healing in adult horses. PLoS One, 19(3), e0299990. https://doi.org/10.1371/journal.pone.0299990

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 19
Issue: 3
Pages: e0299990

Researcher Affiliations

Charlotte C, Paindaveine
  • Unité ICE-Groupe de Recherche en Médecine et Rééducation des Equidés de Sport (GREMERES), Centre for Equine Health, Ecole Nationale Vétérinaire de Lyon, VetAgro Sup, Université de Lyon, Lyon, France.
Benoit, Bihin
  • Namur Research Institute for Life Sciences (NARILIS), University of Namur, Namur, Belgium.
Olivier M, Lepage
  • Unité ICE-Groupe de Recherche en Médecine et Rééducation des Equidés de Sport (GREMERES), Centre for Equine Health, Ecole Nationale Vétérinaire de Lyon, VetAgro Sup, Université de Lyon, Lyon, France.

MeSH Terms

  • Animals
  • Female
  • Epidermis
  • Forelimb
  • Horses
  • Intention
  • Skin Diseases
  • Wound Healing

Conflict of Interest Statement

The authors have declared that no competing interests exist.

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