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Home / Equine Research Bank / Am J Trop Med Hyg / Phylogenetic analysis of eastern equine encephalitis virus i...
The American journal of tropical medicine and hygiene2011; 84(5); 709-717; doi: 10.4269/ajtmh.2011.10-0267

Phylogenetic analysis of eastern equine encephalitis virus isolates from Florida.

Abstract: Florida has the highest degree of endemicity for eastern equine encephalitis virus (EEEV) of any state in the United States and is the only state with year-round transmission of EEEV. To further understand the viral population dynamics in Florida, the genome sequence of six EEEV isolates from central Florida were determined. These data were used to identify the most polymorphic regions of the EEEV genome from viruses isolated in Florida. The sequence of these polymorphic regions was then determined for 18 additional Florida isolates collected in four geographically distinct regions over a 20-year period. Phylogenetic analyses of these data suggested a rough temporal association of the Florida isolates, but no clustering by region or by source of the isolate. Some clustering of northeastern isolates with Florida isolates was seen, providing support for the hypothesis that Florida serves as a reservoir for the periodic introduction of EEEV into the northeastern United States.
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Publication Date: 2011-05-05 PubMed ID: 21540379PubMed Central: PMC3083737DOI: 10.4269/ajtmh.2011.10-0267Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper presents an analysis of the genetic variations in the Eastern equine encephalitis virus (EEEV), with a specific focus on the strains found in Florida. The researchers sequenced the genomes of various virus isolates, identifying the most variable genetic regions and conducting a phylogenetic analysis to study the evolution and distribution patterns of the virus.

Study Setup and Rationale

  • The researchers chose Florida due to its unique status as the most endemic state for EEEV in the United States with year-round transmission.
  • The primary goal of the research was to understand the viral population dynamics of EEEV in Florida.
  • To achieve this, the scientists examined six isolates of the EEEV virus from central Florida and sequenced their genomes.

Identification of Polymorphic Regions

  • By sequencing the genomes of the EEEV isolates, the researchers identified the most polymorphic regions of the EEEV genome, i.e., the areas exhibiting the most genetic variation amongst the viruses.
  • This analysis was further broadened by examining the sequence of these polymorphic regions in 18 additional Florida isolates. These isolates were collected from four different geographical regions over a span of 20 years.

Phylogenetic Analysis and Results

  • Phylogenetic analyses of these data revealed a rough temporal association among the Florida isolates.
  • Interestingly, they found no significant clustering by either region or source of the isolate. This implies that neither geography nor host species significantly influenced the genetic variations observed in the virus.
  • However, some level of clustering of northeastern isolates with Florida isolates was observed. This lends empirical support to the hypothesis that Florida serves as a reservoir for the periodic introduction of EEEV into the northeastern United States.

Impact of the Study

  • This research provides valuable insights into the evolution and genetic variations of the EEEV virus in North America.
  • By identifying Florida as a possible reservoir for the virus, the study could have significant implications for predicting and controlling future outbreaks.

Cite This Article

APA
White GS, Pickett BE, Lefkowitz EJ, Johnson AG, Ottendorfer C, Stark LM, Unnasch TR. (2011). Phylogenetic analysis of eastern equine encephalitis virus isolates from Florida. Am J Trop Med Hyg, 84(5), 709-717. https://doi.org/10.4269/ajtmh.2011.10-0267

Publication

The American journal of tropical medicine and hygiene
ISSN: 1476-1645
NlmUniqueID: 0370507
Country: United States
Language: English
Volume: 84
Issue: 5
Pages: 709-717

Researcher Affiliations

White, Gregory S
  • Global Health Infectious Disease Research Program, Department of Global Health, University of South Florida, 3720 Spectrum Blvd., Tampa, FL 33612, USA. gwhite@cvmvcd.org
Pickett, Brett E
    Lefkowitz, Elliot J
      Johnson, Amelia G
        Ottendorfer, Christy
          Stark, Lillian M
            Unnasch, Thomas R

              MeSH Terms

              • Animals
              • Base Sequence
              • DNA Primers
              • Encephalitis Virus, Eastern Equine / classification
              • Encephalitis Virus, Eastern Equine / genetics
              • Encephalitis Virus, Eastern Equine / isolation & purification
              • Florida
              • Genome, Viral
              • Mice
              • Phylogeny
              • Reverse Transcriptase Polymerase Chain Reaction

              Grant Funding

              • R01 AI049724 / NIAID NIH HHS
              • R01AI049724 / NIAID NIH HHS

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              Citations

              This article has been cited 7 times.
              1. Hughes HR, Velez JO, Davis EH, Laven J, Gould CV, Panella AJ, Lambert AJ, Staples JE, Brault AC. Fatal Human Infection with Evidence of Intrahost Variation of Eastern Equine Encephalitis Virus, Alabama, USA, 2019.. Emerg Infect Dis 2021 Jul;27(7):1886-1892.
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              2. Heberlein-Larson LA, Tan Y, Stark LM, Cannons AC, Shilts MH, Unnasch TR, Das SR. Complex Epidemiological Dynamics of Eastern Equine Encephalitis Virus in Florida.. Am J Trop Med Hyg 2019 May;100(5):1266-1274.
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