Plasma biochemistry in the horse during 3-day event competition.

The research explores the alterations in blood biochemistry in Thoroughbred horses as a result of participation in a 3-day competitive event, and how these changes indicate the physical stressors like dehydration, reduced filtration rate in kidneys, increased glycogen break down, and increased fat metabolism, impacting the horses’ well-being.

Sample Collection and Measurement

• The researchers collected blood samples from 16 Thoroughbred horses before, during, and after the second day of a 3-day event. Through these samples, multiple plasma components were measured and analyzed. These included osmolality, sodium, potassium, chloride, urea, creatinine, glucose, bilirubin, iron, total protein, albumin, several enzymes, calcium, phosphate, uric acid, cholesterol, triglycerides, and non-esterified fatty acids.

Findings and Interpretation

• There were significant shifts noticed in all measured parameters from their pre-event values. The most prominent changes were observed post the cross-country phase of the event.
• The majority of the parameters displayed significant increases after physical exert, except for calcium and chloride, which decreased. This shows that the physical act of these competitive events has tangible impacts on the horses’ body chemistry.
• From these variations in biochemistry, the research team inferred a few physiological responses that were occurring as a result of the competitive exercise. These involved dehydration, reduction in glomerular filtration rate (the process by which the kidneys filter out waste and excess substances from the blood), heightened glycogenolysis (the breakdown of glycogen to glucose for energy), and increased fat metabolism.

Significance of the Study

• This study provides valuable information about the biochemical responses and physical stress that equine athletes endure during competitive events. Such findings can help in the development of better training and recovery strategies to ensure the health and well-being of these horses.