Platelet function, size and yield in whole blood and in platelet-rich plasma prepared using differing centrifugation force and time in domestic and food-producing animals.

This research article explores how different centrifugation forces and times affect platelet function, size, and volume in the blood of various animals, such as bovines, canines, equines, felines, caprines, ovines, and porcines. The study found that platelet yield and sizes vary according to these centrifugation conditions and the animal species.

Methodology and Results

• The study used citrated blood samples from bovine, canine, caprine, equine, feline, ovine and porcine species to compare the effects of differing centrifugation force and time on platelet function, size and volume.
• Among bovine, caprine, feline and ovine species, centrifugation involving longer times and lower gravitational forces led to maximal platelet yields and larger platelet volumes.
• Conversely, in canine, equine and porcine species, shorter centrifugation times and higher gravitational forces resulted in maximal platelet yields and larger platelet volumes.
• Interestingly, platelet aggregation — the clumping together of platelets — to adenosine diphosphate or arachidonic acid remained unaffected by the method of plasma preparation in bovine, caprine, feline, ovine, or porcine platelets.
• However, equine platelet aggregation was highest when plasma was prepared using longer centrifugation times and lower gravitational forces.
• For canine platelets, especially those induced by arachidonic acid, aggregation was maximal when plasma was prepared using short centrifugation times and higher gravitational forces.

Analysis and Conclusion

• The research found that the effects of centrifugation parameters greatly depend on the balance between platelet and red blood cell volumes.
• The researchers concluded that for achieving the maximal platelet yield and volume, the parameters of centrifugation – including time and gravitational force – need to be adjusted according to the specific animal species.
• This detailed understanding of centrifugation parameters could dramatically aid in the preparation of platelet-rich plasma, which is a common component in many medical and veterinary clinical procedures.