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Platelet function, size and yield in whole blood and in platelet-rich plasma prepared using differing centrifugation force and time in domestic and food-producing animals.
Abstract: The effects of centrifugation force and time upon platelets function, mean platelet volume and platelet yield were compared with whole blood platelet counts and size in citrated blood samples from the bovine, canine, caprine, equine, feline, ovine and porcine species. The results were similar, for a given species, irregardless of sample volume. Bovine, caprine, feline and ovine platelet yields and mean platelet volumes were maximal when platelet-rich plasma was prepared using longer centrifugation times and lower gravitational forces. Canine, equine and porcine platelet yields and mean platelet volumes were maximal when platelet-rich plasma was prepared using shorter centrifugation times and higher gravitational forces. Platelet aggregation to adenosine diphosphate or arachidonic acid was not effected by the method of platelet-rich plasma preparation in bovine, caprine, feline, ovine or porcine platelets. Equine platelet aggregation was maximal when platelet-rich plasma was prepared using longer centrifugation times and lower gravitational forces. Canine platelet aggregation, particularly arachidonic acid-induced aggregation, was maximal when platelet-rich plasma was prepared using short centrifugation times and higher gravitational forces. It appeared that the effects of centrifugation parameters upon platelet yield depended upon the relative difference between platelet and red blood cell volumes.
Publication Date: 1983-12-30 PubMed ID: 6665764 The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
- Research Support
- U.S. Gov't
- P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article explores how different centrifugation forces and times affect platelet function, size, and volume in the blood of various animals, such as bovines, canines, equines, felines, caprines, ovines, and porcines. The study found that platelet yield and sizes vary according to these centrifugation conditions and the animal species.
Methodology and Results
- The study used citrated blood samples from bovine, canine, caprine, equine, feline, ovine and porcine species to compare the effects of differing centrifugation force and time on platelet function, size and volume.
- Among bovine, caprine, feline and ovine species, centrifugation involving longer times and lower gravitational forces led to maximal platelet yields and larger platelet volumes.
- Conversely, in canine, equine and porcine species, shorter centrifugation times and higher gravitational forces resulted in maximal platelet yields and larger platelet volumes.
- Interestingly, platelet aggregation — the clumping together of platelets — to adenosine diphosphate or arachidonic acid remained unaffected by the method of plasma preparation in bovine, caprine, feline, ovine, or porcine platelets.
- However, equine platelet aggregation was highest when plasma was prepared using longer centrifugation times and lower gravitational forces.
- For canine platelets, especially those induced by arachidonic acid, aggregation was maximal when plasma was prepared using short centrifugation times and higher gravitational forces.
Analysis and Conclusion
- The research found that the effects of centrifugation parameters greatly depend on the balance between platelet and red blood cell volumes.
- The researchers concluded that for achieving the maximal platelet yield and volume, the parameters of centrifugation – including time and gravitational force – need to be adjusted according to the specific animal species.
- This detailed understanding of centrifugation parameters could dramatically aid in the preparation of platelet-rich plasma, which is a common component in many medical and veterinary clinical procedures.
Cite This Article
APA
Clemmons RM, Bliss EL, Dorsey-Lee MR, Seachord CL, Meyers KM.
(1983).
Platelet function, size and yield in whole blood and in platelet-rich plasma prepared using differing centrifugation force and time in domestic and food-producing animals.
Thromb Haemost, 50(4), 838-843.
Publication
Researcher Affiliations
MeSH Terms
- Animals
- Animals, Domestic / blood
- Blood Platelets
- Cats
- Cattle
- Centrifugation
- Dogs
- Goats
- Horses
- Platelet Aggregation
- Platelet Count
- Platelet Function Tests
- Sheep
- Swine
Grant Funding
- HL-24277-02 / NHLBI NIH HHS
Citations
This article has been cited 9 times.- Valle GD, Alterisio MC, Guccione J, Caterino C, Aragosa F, Ferrara G, De Biase D, Ciaramella P, Fatone G. New frontiers in regenerative medicine: Protocol standardization and morphological assessment of leukocyte-platelet rich fibrin in cows, with a longitudinal study on growth factor release dynamics. Vet Anim Sci 2026 Mar;31:100564.
- Wang ZQ, Lai QW, Gao X, Wu QY, Dong TT, Tsim KW. Platelet-Rich Plasma Extract Derived from Animals Shows Potential in Promoting Wound Healing and Suppressing Inflammatory Response in Skin Cells. Cells 2025 Apr 1;14(7).
- Miguel-Pastor L, Satué K, Chicharro D, Torres-Torrillas M, Del Romero A, Peláez P, Carrillo JM, Cuervo B, Sopena JJ, Cerón JJ, Rubio M. Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study. Front Vet Sci 2022;9:866547.
- Vikartovska Z, Kuricova M, Farbakova J, Liptak T, Mudronova D, Humenik F, Madari A, Maloveska M, Sykova E, Cizkova D. Stem Cell Conditioned Medium Treatment for Canine Spinal Cord Injury: Pilot Feasibility Study. Int J Mol Sci 2020 Jul 20;21(14).
- Pandey S, Hickey DU, Drum M, Millis DL, Cekanova M. Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro. BMC Vet Res 2019 Jul 30;15(1):269.
- Kiapour AM, Shalvoy MR, Murray MM, Fleming BC. Validation of porcine knee as a sex-specific model to study human anterior cruciate ligament disorders. Clin Orthop Relat Res 2015 Feb;473(2):639-50.
- Perazzi A, Busetto R, Martinello T, Drigo M, Pasotto D, Cian F, Patruno M, Iacopetti I. Description of a double centrifugation tube method for concentrating canine platelets. BMC Vet Res 2013 Jul 22;9:146.
- Choi W, Karim ZA, Whiteheart SW. Protein expression in platelets from six species that differ in their open canalicular system. Platelets 2010;21(3):167-75.
- Spindler KP, Murray MM, Carey JL, Zurakowski D, Fleming BC. The use of platelets to affect functional healing of an anterior cruciate ligament (ACL) autograft in a caprine ACL reconstruction model. J Orthop Res 2009 May;27(5):631-8.
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