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The Journal of veterinary medical science2002; 63(11); 1191-1197; doi: 10.1292/jvms.63.1191

Population study and validation of paternity testing for Thoroughbred horses by 15 microsatellite loci.

Abstract: Microsatellite 15 TKY System was characterized for parentage verification of horse registry. The Microsatellite 15 TKY System was constructed by using 15 microsatellites, TKY279, TKY287, TKY294, TKY297, TKY301, TKY312, TKY321, TKY325, TKY333, TKY337, TKY341, TKY343, TKY344, TKY374, and TKY394, to provide stringent PCR-based microsatellite typing specifically optimized for multicolor fluorescence detection. The Microsatellite 15 TKY System showed good resolutions for 250 unrelated Thoroughbred horses, and the probability of exclusion (PE) at each microsatellite ranged from 0.437 to 0.621, resulting in a total PE value of 99.998% for Thoroughbred horses. These results indicated that the Microsatellite 15 TKY System is useful for paternity testing of Thoroughbred horses. A paternity testing case for a Thoroughbred horse family, in which candidate sires had close relations, was analyzed using the Microsatellite 15 TKY System. In this case, the Microsatellite 15 TKY System excluded paternity of a false sire. We concluded that the Microsatellite 15 TKY System can give sufficient and reliable information for paternity testing.
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Publication Date: 2002-01-05 PubMed ID: 11767052DOI: 10.1292/jvms.63.1191Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Validation Study

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study developed and validated a technique for confirming the paternity of Thoroughbred horses using 15 specific microsatellite markers. The method was shown to be both reliable and effective.

Research Methodology

  • The researchers developed a method for paternity verification in horse registry using a Microsatellite 15 TKY System, created using 15 specific microsatellites. These microsatellites are short, repetitive DNA sequences that are distributed throughout the genome. They’re highly variable between individuals, making them helpful in genetic identification and paternity testing.
  • The technique developed used a PCR-based microsatellite typing. PCR, or Polymerase Chain Reaction, is a technique used in molecular biology to amplify a single or few copies of a piece of DNA. Using this mechanism, the researchers could make millions to billions of copies of a particular DNA sequence.
  • The Microsatellite 15 TKY System was specifically optimized for multicolor fluorescence detection, a method of observing different color fluorescence to visualize and quantify various biological processes.

Testing and Results

  • The researchers tested the method’s efficiency on 250 unrelated Thoroughbred horses. The system showed good resolutions, meaning it effectively differentiated between horses.
  • The system provided a probability of exclusion (PE) value for each microsatellite, ranging from 0.437 to 0.621. Probability of Exclusion is a measure to determine the statistical confidence to exclude a falsely accused genetic parent.
  • The combined PE value for all 15 microsatellites was 99.998%, indicating an extremely high level of reliability for paternity testing of Thoroughbred horses.

Validation and Conclusion

  • The researchers validated the system by using it for a paternity testing case for a Thoroughbred horse family where the candidate sires had close relations. The Microsatellite 15 TKY System successfully excluded the paternity of the false sire.
  • The study concluded that the Microsatellite 15 TKY System can provide sufficient and reliable information for paternity testing. Hence, it is a useful tool for parentage verification in horse registries.

Cite This Article

APA
Tozaki T, Kakoi H, Mashima S, Hirota K, Hasegawa T, Ishida N, Miura N, Choi-Miura NH, Tomita M. (2002). Population study and validation of paternity testing for Thoroughbred horses by 15 microsatellite loci. J Vet Med Sci, 63(11), 1191-1197. https://doi.org/10.1292/jvms.63.1191

Publication

The Journal of veterinary medical science
ISSN: 0916-7250
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 63
Issue: 11
Pages: 1191-1197

Researcher Affiliations

Tozaki, T
  • Department of Molecular Genetics, Laboratory of Racing Chemistry, Utsunomiya, Tochigi, Japan.
Kakoi, H
    Mashima, S
      Hirota, K
        Hasegawa, T
          Ishida, N
            Miura, N
              Choi-Miura, N H
                Tomita, M

                  MeSH Terms

                  • Animals
                  • DNA / chemistry
                  • DNA / genetics
                  • DNA / isolation & purification
                  • Female
                  • Horses / genetics
                  • Male
                  • Microsatellite Repeats / genetics
                  • Paternity
                  • Pedigree
                  • Polymerase Chain Reaction / methods
                  • Polymerase Chain Reaction / veterinary
                  • Polymorphism, Genetic / genetics

                  Citations

                  This article has been cited 25 times.
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