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International journal of molecular sciences2023; 24(18); 14169; doi: 10.3390/ijms241814169

Pro-Inflammatory Cytokine Priming and Purification Method Modulate the Impact of Exosomes Derived from Equine Bone Marrow Mesenchymal Stromal Cells on Equine Articular Chondrocytes.

Abstract: Osteoarthritis (OA) is a widespread osteoarticular pathology characterized by progressive hyaline cartilage degradation, exposing horses to impaired well-being, premature career termination, alongside substantial financial losses for horse owners. Among the new therapeutic strategies for OA, using mesenchymal stromal cell (MSC)-derived exosomes (MSC-exos) appears to be a promising option for conveying MSC therapeutic potential, yet avoiding the limitations inherent to cell therapy. Here, we first purified and characterized exosomes from MSCs by membrane affinity capture (MAC) and size-exclusion chromatography (SEC). We showed that intact MSC-exos are indeed internalized by equine articular chondrocytes (eACs), and then evaluated their functionality on cartilaginous organoids. Compared to SEC, mRNA and protein expression profiles revealed that MAC-exos induced a greater improvement of eAC-neosynthesized hyaline-like matrix by modulating collagen levels, increasing PCNA, and decreasing Htra1 synthesis. However, because the MAC elution buffer induced unexpected effects on eACs, an ultrafiltration step was included to the isolation protocol. Finally, exosomes from MSCs primed with equine pro-inflammatory cytokines (IL-1β, TNF-α, or IFN-γ) further improved the eAC hyaline-like phenotype, particularly IL-1β and TNF-α. Altogether, these findings indicate the importance of the exosome purification method and further demonstrate the potential of pro-inflammatory priming in the enhancement of the therapeutic value of MSC-exos for equine OA treatment.
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Publication Date: 2023-09-16 PubMed ID: 37762473PubMed Central: PMC10531906DOI: 10.3390/ijms241814169Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper discusses the use of exosomes derived from mesenchymal stromal cells (MSCs) in suiting up equine osteoarthritis (OA) treatment, detailing their purification, examining their functionality, effect on hyaline-like matrix synthesis, and the value of pro-inflammatory cytokine priming.

Research Methodology

  • Initially, the researchers focused on the purification and characterization of exosomes obtained from MSCs. The methods utilized for this process were membrane affinity capture (MAC) and size-exclusion chromatography (SEC).
  • Following this, they assessed that these exosomes were indeed being assimilated by equine articular chondrocytes (eACs), which are the cells potentially affected in OA.
  • The functionality these exosomes had on cartilaginous organoids was then inspected.

Findings and Interpretation

  • A comparison between the two purification methods showed that exosomes purified using MAC (MAC-exos) seemed to encourage a superior enhancement of the hyaline-like matrix (which is important to cartilage structure) that eACs newly synthesize. They achieved this by controlling collagen levels, boosting the PCNA (Proliferating Cell Nuclear Antigen), and decreasing the synthesis of Htra1, a protein known to degrade extracellular matrix.
  • Unexpected side effects were, however, noticed due to the MAC elution buffer which forced the inclusion of an ultrafiltration phase into the isolation protocol.
  • Further studies revealed that the use of exosomes from MSCs, which were primed with horse-derived pro-inflammatory cytokines (messengers such as IL-1β, TNF-α, IFN-γ that contribute to cell signaling), showed considerable improvement in the hyaline-like matrix synthesis by the eACs, thereby showing potential for increased effectiveness of the treatment for OA. In particular, IL-1β and TNF-α showed the most improvement.

Conclusion

  • The study provides novel findings regarding the purification method of exosomes from MSCs that enhances their therapeutic potential for treating equine OA, with implications for the role of pro-inflammatory priming.
  • The exploration of these conditions and variables seeks to optimize their therapeutic value, outlining a promising pathway for future treatments for OA in horses.

Cite This Article

APA
Jammes M, Cassé F, Velot E, Bianchi A, Audigié F, Contentin R, Galéra P. (2023). Pro-Inflammatory Cytokine Priming and Purification Method Modulate the Impact of Exosomes Derived from Equine Bone Marrow Mesenchymal Stromal Cells on Equine Articular Chondrocytes. Int J Mol Sci, 24(18), 14169. https://doi.org/10.3390/ijms241814169

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 24
Issue: 18
PII: 14169

Researcher Affiliations

Jammes, Manon
  • BIOTARGEN, UNICAEN, Normandie University, 14000 Caen, France.
Cassé, Frédéric
  • BIOTARGEN, UNICAEN, Normandie University, 14000 Caen, France.
Velot, Emilie
  • Molecular Engineering and Articular Physiopathology (IMoPA), French National Center for Scientific Research (CNRS), Université de Lorraine, 54000 Nancy, France.
Bianchi, Arnaud
  • Molecular Engineering and Articular Physiopathology (IMoPA), French National Center for Scientific Research (CNRS), Université de Lorraine, 54000 Nancy, France.
Audigié, Fabrice
  • Center of Imaging and Research in Locomotor Affections on Equines, Veterinary School of Alfort, 14430 Goustranville, France.
Contentin, Romain
  • BIOTARGEN, UNICAEN, Normandie University, 14000 Caen, France.
Galéra, Philippe
  • BIOTARGEN, UNICAEN, Normandie University, 14000 Caen, France.

MeSH Terms

  • Horses
  • Animals
  • Chondrocytes
  • Cytokines
  • Tumor Necrosis Factor-alpha
  • Bone Marrow
  • Exosomes
  • Osteoarthritis / therapy
  • Osteoarthritis / veterinary
  • Mesenchymal Stem Cells

Conflict of Interest Statement

The authors declare no conflict of interest.

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