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The open biochemistry journal2011; 5; 52-59; doi: 10.2174/1874091X01105010052

Production of free radicals and oxygen consumption by primary equine endothelial cells during anoxia-reoxygenation.

Abstract: The endothelium plays an active role in ischemia/reperfusion injuries. Herein, we report the effect of a single or successive cycles of anoxia/reoxygenation (A/R) on the mitochondrial respiratory function of equine endothelial cells (cultured from carotids) monitored by high resolution oxymetry, and on their production of reactive oxygen species (ROS). ROS were measured by electron paramagnetic resonance (ESR) using POBN and DMPO spin traps, and by gas chromatography (GC) of ethylene released by ROS-induced α-keto-γ-(methylthio)butyric acid (KMB) oxidation. The oxygen consumption significantly decreased with the number of A/R cycles, and POBN-ESR spectra were specific of adducts formed in the cells from superoxide anion. After a one-hour A/R cycle, high intensity DMPO-ESR spectra were observed and assigned to superoxide anion trapping; the GC results confirmed an important production of ROS compared to normoxic cells. These results show that A/R induces mitochondrial alterations in endothelial cells, and strongly stimulates their oxidative activity as demonstrated by ESR and GC methods.
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Publication Date: 2011-11-24 PubMed ID: 22207886PubMed Central: PMC3242399DOI: 10.2174/1874091X01105010052Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated the impact of low oxygen and reoxygenation conditions (anoxia/reoxygenation) on horse endothelial cells’ mitochondrial function and their production of harmful reactive oxygen species. The results show that these conditions caused mitochondrial damages in endothelial cells and drastically increased their oxidative activity.

Research Context

  • The endothelium, a layer of cells lining the interior of blood vessels, plays a crucial role in injuries caused by a cycle of tissue oxygen deprivation (ischemia) and restoration (reperfusion).
  • This research aimed to understand the effects of single and successive cycles of anoxia/reoxygenation (A/R) on equine endothelial cells, specifically how it alters their mitochondrial function and the production of reactive oxygen species (ROS).

Methods and Observations

  • The endothelial cells, cultured from horse carotids, underwent A/R cycles. Their mitochondrial function was monitored using high-resolution oxymetry, an instrument that measures oxygen concentration.
  • The generation of ROS in the cells was measured via electron paramagnetic resonance (ESR) and gas chromatography (GC).
  • During A/R cycles, the oxygen consumption of the cells fell significantly.
  • The ESR spectra were found to be unique for adducts (a molecule formed from the direct combination of two other molecules) resulting from a harmful form of oxygen called the superoxide anion.
  • After a one-hour A/R cycle, high intensity ESR spectra were seen which were attributed to the trapping of the superoxide anion. GC results corroborated a significant increase in ROS production as compared to cells kept under normal oxygenation (normoxic cells).

Conclusion

  • These findings demonstrated that A/R cycles induced harmful alterations in endothelial cells’ mitochondria, enhancing their oxidative activity significantly. The research methods of ESR and GC were crucial to these observations.

Cite This Article

APA
de Rebière de Pouyade G, Salciccia A, Ceusters J, Deby-Dupont G, Serteyn D, Mouithys-Mickalad A. (2011). Production of free radicals and oxygen consumption by primary equine endothelial cells during anoxia-reoxygenation. Open Biochem J, 5, 52-59. https://doi.org/10.2174/1874091X01105010052

Publication

The open biochemistry journal
ISSN: 1874-091X
NlmUniqueID: 101471222
Country: United Arab Emirates
Language: English
Volume: 5
Pages: 52-59

Researcher Affiliations

de Rebière de Pouyade, Geoffroy
  • Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Sart Tilman, 4000 Liège, Belgium.
Salciccia, Alexandra
    Ceusters, Justine
      Deby-Dupont, Ginette
        Serteyn, Didier
          Mouithys-Mickalad, Ange

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