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Home / Equine Research Bank / J Equine Sci / Profiling of exercise-induced transcripts in the peripheral ...
Journal of equine science2016; 27(4); 157-164; doi: 10.1294/jes.27.157

Profiling of exercise-induced transcripts in the peripheral blood cells of Thoroughbred horses.

Abstract: Transcriptome analyses based on DNA microarray technology have been used to investigate gene expression profiles in horses. In this study, we aimed to identify exercise-induced changes in the expression profiles of genes in the peripheral blood of Thoroughbred horses using DNA microarray technology (15,429 genes on 43,603 probes). Blood samples from the jugular vein were collected from six horses before and 1 min, 4 hr, and 24 hr after all-out running on a treadmill. After the normalization of microarray data, a total of 26,830 probes were clustered into four groups and 11 subgroups showing similar expression changes based on k-mean clustering. The expression level of inflammation-related genes, including interleukin-1 receptor type II (IL-1R2), matrix metallopeptidase 8 (MMP8), protein S100-A8 (S100-A8), and serum amyloid A (SAA), increased at 4 hr after exercise, whereas that of c-Fos (FOS) increased at 1 min after exercise. These results indicated that the inflammatory response increased in the peripheral blood cells after exercise. Our study also revealed the presence of genes that may not be affected by all-out exercise. In conclusion, transcriptome analysis of peripheral blood cells could be used to monitor physiological changes induced by various external stress factors, including exercise, in Thoroughbred racehorses.
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Publication Date: 2016-12-15 PubMed ID: 27974875PubMed Central: PMC5155134DOI: 10.1294/jes.27.157Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines changes in the gene expression profiles in the peripheral blood of Thoroughbred horses after exercise, using DNA microarray technology. The findings highlight that exercise induces an inflammatory response in the horses’ blood cells.

Objective of the Research

  • The main aim of the research was to profile exercise-induced changes in the expression of genes in the peripheral blood of Thoroughbred horses. The researchers used DNA microarray technology to achieve this, exploring a total of 15,429 genes on 43,603 probes.

Methodology

  • The researchers collected blood samples from the jugular vein of six horses, taken before and at 1 minute, 4 hours, and 24 hours after all-out running on a treadmill.
  • After normalizing the microarray data collected, the researchers clustered 26,830 probes into four groups and eleven subgroups. This clustering was based on similar expression changes identified using k-mean clustering.

Key Findings

  • The study found that the expression level of certain inflammation-related genes, including Interleukin-1 Receptor Type II (IL-1R2), Matrix Metallopeptidase 8 (MMP8), Protein S100-A8 (S100-A8), and Serum Amyloid A (SAA), increased 4 hours post-exercise.
  • The gene c-Fos (FOS) showed an increased expression level just 1 minute after exercise.
  • The researchers noticed that these results indicate an increased inflammatory response in the peripheral blood cells post-exercise.
  • The study also identified genes that may remain unaffected by all-out exercise.

Conclusion

  • The authors concluded on the note that transcriptome analysis of peripheral blood cells can be effective in monitoring physiological changes induced by various external stress factors, specifically exercise, in Thoroughbred racehorses.

Cite This Article

APA
Tozaki T, Kikuchi M, Kakoi H, Hirota KI, Mukai K, Aida H, Nakamura S, Nagata SI. (2016). Profiling of exercise-induced transcripts in the peripheral blood cells of Thoroughbred horses. J Equine Sci, 27(4), 157-164. https://doi.org/10.1294/jes.27.157

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 27
Issue: 4
Pages: 157-164

Researcher Affiliations

Tozaki, Teruaki
  • Genetic Analysis Department, Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
Kikuchi, Mio
  • Genetic Analysis Department, Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
Kakoi, Hironaga
  • Genetic Analysis Department, Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
Hirota, Kei-Ichi
  • Genetic Analysis Department, Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
Mukai, Kazutaka
  • Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
Aida, Hiroko
  • Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
Nakamura, Seiji
  • DNA Chip Research Inc., Tokyo 105-0022, Japan.
Nagata, Shun-Ichi
  • Genetic Analysis Department, Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.

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Citations

This article has been cited 1 times.
  1. Minamijima Y, Niwa H, Uchida E, Yamamoto K. Comparison of the proteomes in sera between healthy Thoroughbreds and Thoroughbreds with respiratory disease associated with transport using mass spectrometry-based proteomics.. J Equine Sci 2021 Mar;32(1):11-15.
    doi: 10.1294/jes.32.11pubmed: 33776535google scholar: lookup
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