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Home / Equine Research Bank / Can J Vet Res / Prostaglandin E2 and reactive oxygen metabolite damage in th...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire2002; 66(1); 50-54;

Prostaglandin E2 and reactive oxygen metabolite damage in the cecum in a pony model of acute colitis.

Abstract: The objective of this project was to determine early tissue biochemical events associated with increased colonic secretion during the acute stage of castor-oil-induced colitis by measuring cecal mucosal and submucosal malondialdehyde (MDA) and prostaglandin E2 (PGE2), levels in ponies. Intestinal tissue (inflamed or healthy) samples were obtained from 4 age- and sex-matched Shetland ponies. Biochemical methods were used to determine MDA and PGE2 levels in intestinal tissue samples from inflamed and healthy equine intestine. Inflamed tissue MDA and PGE2 levels increased with time after castor oil challenge and correlated with granulocyte infiltration, as determined by myeloperoxidase levels in a companion study. Elevated intestinal tissue MDA levels suggest that lipid peroxidation could be attributed to reactive oxygen metabolites (ROM) released from stimulated, recruited, and resident granulocytes. Tissue levels of MDA and PGE2 suggest a role for granulocyte-derived mediators of intestinal inflammation in the massive secretory response in cases of acute equine colitis. Tissue MDA and PGE2 levels may be useful laboratory tools to quantify and characterize intestinal secretory inflammatory responses in acute inflammatory conditions in the equine colon.
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Publication Date: 2002-02-23 PubMed ID: 11858649PubMed Central: PMC226982
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aims to understand the associations between increased colon secretion during acute colitis in ponies by observing the levels of cecal mucosal and submucosal malondialdehyde (MDA) and prostaglandin E2 (PGE2). A discovery of lipid peroxidation, attributed to reactive oxygen metabolites (ROM), and evidence of granulocyte-driven inflammation are indicated.

Objective and Methodology

  • The study aimed to explore early tissue chemical reactions associated with increased colonic secretion during the acute stage of colitis induced by castor oil.
  • The research targeted these biochemical events by measuring levels of cecal mucosal and submucosal malondialdehyde (MDA) and prostaglandin E2 (PGE2).
  • These measurements were made using intestinal tissue samples, both inflamed and healthy, from four Shetland ponies matched for age and sex.
  • Biochemical methods were used to determine MDA and PGE2 levels in these tissue samples.

Results and Findings

  • Levels of MDA and PGE2 in inflamed tissue samples were found to rise over time following a castor oil challenge.
  • These increases were correlated with granulocyte infiltration – as determined through measuring myeloperoxidase levels in a parallel study.
  • Elevated levels of MDA in intestinal tissues suggest that lipid peroxidation might be due to ROM (reactive oxygen metabolites) released from both resident and recruited granulocytes that have been stimulated.
  • The findings provide evidence for a role for granulocyte-derived mediators in the intestinal inflammation seen in acute equine colitis.

Implications of the Study

  • Levels of tissue MDA and PGE2 could offer a means to quantify and characterize intestinal secretory inflammatory responses in acute inflammatory conditions of the equine colon.
  • This study provides valuable insights in understanding the biochemical reactions that are associated with the inflammation in cases of equine colitis and could guide in the formulation of more effective treatments for this condition.

Cite This Article

APA
McConnico RS, Argenzio RA, Roberts MC. (2002). Prostaglandin E2 and reactive oxygen metabolite damage in the cecum in a pony model of acute colitis. Can J Vet Res, 66(1), 50-54.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 0830-9000
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 66
Issue: 1
Pages: 50-54

Researcher Affiliations

McConnico, Rebecca S
  • Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA. mcconnico@vetmed.lsu.edu
Argenzio, Robert A
    Roberts, Malcolm C

      MeSH Terms

      • Acute Disease
      • Animals
      • Castor Oil / adverse effects
      • Cecum / metabolism
      • Cecum / pathology
      • Colitis / chemically induced
      • Colitis / metabolism
      • Colitis / pathology
      • Colitis / veterinary
      • Dinoprostone / analysis
      • Dinoprostone / metabolism
      • Disease Models, Animal
      • Female
      • Granulocytes / immunology
      • Horse Diseases / chemically induced
      • Horse Diseases / metabolism
      • Horse Diseases / pathology
      • Horses
      • Inflammation / veterinary
      • Intestinal Mucosa / chemistry
      • Intestinal Mucosa / immunology
      • Intestinal Mucosa / pathology
      • Lipid Peroxidation
      • Male
      • Malondialdehyde / analysis
      • Malondialdehyde / metabolism
      • Peroxidase / metabolism
      • Reactive Oxygen Species

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