Proteomic alteration of equine monocyte-derived macrophages infected with equine infectious anemia virus.
Abstract: Similar to the well-studied viruses human immunodeficiency virus (HIV)-1 and simian immunodeficiency virus (SIV), equine infectious anemia virus (EIAV) is another member of the Lentivirus genus in the family Retroviridae. Previous studies revealed that interactions between EIAV and the host resulted in viral evolution in pathogenicity and immunogenicity, as well as adaptation to the host. Proteomic analysis has been performed to examine changes in protein expression and/or modification in host cells infected with viruses and has revealed useful information for virus-host interactions. In this study, altered protein expression in equine monocyte-derived macrophages (eMDMs, the principle target cell of EIAV in vivo) infected with the EIAV pathogenic strain EIAV(DLV34) (DLV34) was examined using 2D-LC-MS/MS coupled with the iTRAQ labeling technique. The expression levels of 210 cellular proteins were identified to be significantly upregulated or downregulated by infection with DLV34. Alterations in protein expression were confirmed by examining the mRNA levels of eight selected proteins using quantitative real-time reverse-transcription PCR, and by verifying the levels of ten selected proteins using parallel reaction monitoring (PRM). Further analysis of GO and Kyoto Encyclopedia of Genes and Genomes (KEGG)-Pathway enrichment demonstrated that these differentially expressed proteins are primarily related to the biological processes of oxidative phosphorylation, protein folding, RNA splicing, and ubiquitylation. Our results can facilitate a better understanding of the host response to EIAV infection and the cellular processes required for EIAV replication and pathogenesis.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication Date: 2015-03-21 PubMed ID: 25684102DOI: 10.1002/pmic.201400279Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research is about investigating how equine infectious anemia virus (EIAV), similar to HIV and SIV, affects the protein expression in equine monocyte-derived macrophages (eMDMs), the main target cells of EIAV in the body. Using multiple techniques, 210 proteins were identified to have altered expression levels, contributing to our understanding of EIAV’s impact and providing insight into virus-host interactions.
Methodology and Key Findings
- The researchers utilized a combination of biochemical techniques including 2D-LC-MS/MS (a type of mass spectrometry) and iTRAQ labeling (a chemical labeling method) to study the alterations in protein expression in eMDMs infected with EIAV.
- These methodologies identified 210 proteins whose expression levels were either upregulated (increased) or downregulated (decreased) due to EIAV infection.
- To validate these results, the researchers used quantitative real-time reverse-transcription PCR to analyze the mRNA levels of eight selected proteins. mRNA levels usually correlate with protein production, so this allowed them to confirm the altered protein expression.
- They also used parallel reaction monitoring (PRM), another form of mass spectrometry, to verify the levels of ten additional selected proteins.
Significance and Implications
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG)-Pathway enrichment analysis showed that the differentially expressed proteins are primarily involved in several biological processes. These include oxidative phosphorylation (an energy production process), protein folding (how a protein shape achieves its functional conformation), RNA splicing (processing of pre-mRNA), and ubiquitylation (a process regulating protein degradation).
- This information provides a greater understanding of the cellular mechanisms impacted by EIAV and the processes utilized by this virus for its replication and pathogenesis.
- By understanding these virus-host interactions, therapeutic targets can be identified for treating diseases caused by lentiviruses like EIAV. Furthermore, this research may also provide valuable insight for diseases caused by other similar viruses, such as HIV-1 and SIV.
Cite This Article
APA
Du C, Liu HF, Lin YZ, Wang XF, Ma J, Li YJ, Wang X, Zhou JH.
(2015).
Proteomic alteration of equine monocyte-derived macrophages infected with equine infectious anemia virus.
Proteomics, 15(11), 1843-1858.
https://doi.org/10.1002/pmic.201400279 Publication
Researcher Affiliations
- State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
- Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, P. R. China.
- State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
- State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
- State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
- State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
- Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, P. R. China.
- State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
- State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P. R. China.
- Hayao Pharmaceutical Group Biovaccine Co, Harbin, P. R. China.
MeSH Terms
- Amino Acid Sequence
- Animals
- Cells, Cultured
- Equine Infectious Anemia / metabolism
- Gene Ontology
- Horses
- Host-Pathogen Interactions
- Infectious Anemia Virus, Equine / pathogenicity
- Macrophages / metabolism
- Macrophages / virology
- Molecular Sequence Data
- Proteome / analysis
- Proteome / genetics
- Proteome / metabolism
- Reproducibility of Results
- Reverse Transcriptase Polymerase Chain Reaction / methods
- Tandem Mass Spectrometry
- Virus Replication
Citations
This article has been cited 10 times.- Zou D, Coudron TA, Wu H, Zhang L, Wang M, Xu W, Xu J, Song L, Xiao X. Differential Proteomics Analysis Unraveled Mechanisms of Arma chinensis Responding to Improved Artificial Diet. Insects 2022 Jul 2;13(7).
- Yi W, Chen C, Gan X. Polymyxin B(1) and E(2) From Paenibacillus polymyxa Y-1 for Controlling Rice Bacterial Disease. Front Cell Infect Microbiol 2022;12:866357.
- Du C, Duan Y, Wang XF, Lin Y, Na L, Wang X, Chen K, Wang X. Attenuation of Equine Lentivirus Alters Mitochondrial Protein Expression Profile from Inflammation to Apoptosis. J Virol 2019 Nov 1;93(21).
- Guo M, Zhang X, Li M, Li T, Duan X, Zhang D, Hu L, Huang R. Label-Free Proteomic Analysis of Molecular Effects of 2-Methoxy-1,4-naphthoquinone on Penicillium italicum. Int J Mol Sci 2019 Jul 14;20(14).
- Wei B, Yang Z, Cheng Y, Zhou J, Yang H, Zhang L, Yang X. Proteomic Analysis of the Hepatopancreas of Chinese Mitten Crabs (Eriocheir sinensis) Fed With a Linoleic Acid or α-Linolenic Acid Diet. Front Physiol 2018;9:1430.
- Peterson TA, MacLean AG. Current and Future Therapeutic Strategies for Lentiviral Eradication from Macrophage Reservoirs. J Neuroimmune Pharmacol 2019 Mar;14(1):68-93.
- Wei B, Yang Z, Cheng Y, Wang J, Zhou J. Effects of the complete replacement of fish oil with linseed oil on growth, fatty acid composition, and protein expression in the Chinese mitten crab (Eriocheir sinensis). Proteome Sci 2018;16:6.
- Wang Z, Shang P, Li Q, Wang L, Chamba Y, Zhang B, Zhang H, Wu C. iTRAQ-based proteomic analysis reveals key proteins affecting muscle growth and lipid deposition in pigs. Sci Rep 2017 Apr 24;7:46717.
- Du J, Xing S, Tian Z, Gao S, Xie J, Chang H, Liu G, Luo J, Yin H. Proteomic analysis of sheep primary testicular cells infected with bluetongue virus. Proteomics 2016 May;16(10):1499-514.
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