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Home / Equine Research Bank / J Proteomics / Proteomic characterization and cross species comparison of m...
Journal of proteomics2013; 91; 13-22; doi: 10.1016/j.jprot.2013.05.029

Proteomic characterization and cross species comparison of mammalian seminal plasma.

Abstract: Seminal plasma contains a large protein component which has been implicated in the function, transit and survival of spermatozoa within the female reproductive tract. However, the identity of the majority of these proteins remains unknown and a direct comparison between the major domestic mammalian species has yet to be made. As such, the present study characterized and compared the seminal plasma proteomes of cattle, horse, sheep, pig, goat, camel and alpaca. GeLC-MS/MS and shotgun proteomic analysis by 2D-LC-MS/MS identified a total of 302 proteins in the seminal plasma of the chosen mammalian species. Nucleobindin 1 and RSVP14, a member of the BSP (binder of sperm protein) family, were identified in all species. Beta nerve growth factor (bNGF), previously identified as an ovulation inducing factor in alpacas and llamas, was identified in this study in alpaca and camel (induced ovulators), cattle, sheep and horse (spontaneous ovulators) seminal plasma. These findings indicate that while the mammalian species studied have common ancestry as ungulates, their seminal plasma is divergent in protein composition, which may explain variation in reproductive capacity and function. The identification of major specific proteins within seminal plasma facilitates future investigation of the role of each protein in mammalian reproduction. Conclusions: This proteomic study is the first study to compare the protein composition of seminal plasma from seven mammalian species including two camelid species. Beta nerve growth factor, previously described as the ovulation inducing factor in camelids is shown to be the major protein in alpaca and camel seminal plasma and also present in small amounts in bull, ram, and horse seminal plasma.
Copyright © 2013. Published by Elsevier B.V.
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Publication Date: 2013-06-06 PubMed ID: 23748023DOI: 10.1016/j.jprot.2013.05.029Google Scholar: Lookup
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  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the protein components of seminal plasma found in several mammalian species, with the aim of identifying these proteins and comparing them across species. The presence of specific proteins, like beta nerve growth factor (bNGF), was observed across different species and is being linked to their reproductive function.

Research Methodology

  • In order to identify and compare the proteins in the seminal plasma of various mammalian species, the researchers employed GeLC-MS/MS and shotgun proteomic analysis by 2D-LC-MS/MS.
  • Mammalian species involved in the research included cattle, horse, sheep, pig, goat, camel, and alpaca.
  • A total of 302 proteins were identified from the seminal plasma of these species.

Key Findings

  • Two proteins, Nucleobindin 1 and RSVP14 (a member of the Binder of Sperm Protein or BSP family), were found across all species.
  • Another protein, beta nerve growth factor (bNGF), was found in the seminal plasma of alpaca, camel (which are known as induced ovulators), and in spontaneous ovulators like cattle, sheep, and horse. bNGF was previously identified as an ovulation inducing factor in alpacas and llamas.
  • This discovery suggests that while all the researched mammalian species share common ancestry as ungulates, there is a divergence in the protein composition of their seminal plasma.

Implications

  • The differences in protein composition in the seminal plasma could potentially explain variations in reproductive capacity and function across different mammalian species.
  • Identifying major proteins in the seminal plasma could pave the way for future investigations into how each protein influences mammalian reproduction.
  • This proteomic research is the first study of its kind to compare the protein composition of seminal plasma across seven different mammalian species, including two camelid species.
  • The study reveals that beta nerve growth factor, which was previously described as the ovulation inducing factor in camelids, is a major protein in the seminal plasma of alpaca and camel, and also present in small quantity in bull, ram, and horse seminal plasma.

Cite This Article

APA
Druart X, Rickard JP, Mactier S, Kohnke PL, Kershaw-Young CM, Bathgate R, Gibb Z, Crossett B, Tsikis G, Labas V, Harichaux G, Grupen CG, de Graaf SP. (2013). Proteomic characterization and cross species comparison of mammalian seminal plasma. J Proteomics, 91, 13-22. https://doi.org/10.1016/j.jprot.2013.05.029

Publication

Journal of proteomics
ISSN: 1876-7737
NlmUniqueID: 101475056
Country: Netherlands
Language: English
Volume: 91
Pages: 13-22
PII: S1874-3919(13)00305-9

Researcher Affiliations

Druart, X
  • INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France; CNRS, UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours, F-37000 Tours, France; IFCE, F-37380 Nouzilly, France. Electronic address: Xavier.Druart@tours.inra.fr.
Rickard, J P
    Mactier, S
      Kohnke, P L
        Kershaw-Young, C M
          Bathgate, R
            Gibb, Z
              Crossett, B
                Tsikis, G
                  Labas, V
                    Harichaux, G
                      Grupen, C G
                        de Graaf, S P

                          MeSH Terms

                          • Animals
                          • Calcium-Binding Proteins / metabolism
                          • Camelids, New World
                          • Camelus
                          • Cattle
                          • DNA-Binding Proteins / metabolism
                          • Gene Expression Regulation
                          • Glycoproteins / metabolism
                          • Goats
                          • Horses
                          • Male
                          • Nerve Growth Factor / metabolism
                          • Nerve Tissue Proteins / metabolism
                          • Nucleobindins
                          • Phylogeny
                          • Proteomics
                          • Semen / metabolism
                          • Seminal Plasma Proteins / metabolism
                          • Sheep
                          • Species Specificity
                          • Swine

                          Citations

                          This article has been cited 39 times.
                          1. Ďuračka M, Benko F, Tvrdá E. Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability.. Int J Mol Sci 2023 Feb 8;24(4).
                            doi: 10.3390/ijms24043379pubmed: 36834790google scholar: lookup
                          2. Gouletsou PG, Tsangaris GT, Katsarou EI, Bourganou MV, Barbagianni MS, Venianaki AP, Bouroutzika E, Anagnostopoulos AK, Fthenakis GC, Katsafadou AI. Proteomics Evaluation of Semen of Clinically Healthy Beagle-Breed Dogs.. Vet Sci 2022 Dec 15;9(12).
                            doi: 10.3390/vetsci9120697pubmed: 36548858google scholar: lookup
                          3. Ferraguti G, Fanfarillo F, Tarani L, Blaconà G, Tarani F, Barbato C, Minni A, Ralli M, Francati S, Greco A, Petrella C, Fiore M. NGF and the Male Reproductive System: Potential Clinical Applications in Infertility.. Int J Mol Sci 2022 Oct 28;23(21).
                            doi: 10.3390/ijms232113127pubmed: 36361912google scholar: lookup
                          4. Senra RL, Ramírez-López CJ, Magalhães-Júnior MJ, Neves JGDS, Barros E, Waddington B, Guimarães SEF, Guimarães JD, Baracat-Pereira MC. Kallikrein proteoforms and reproductive parameters in stallion are conditioned by climate.. Sci Rep 2022 Nov 4;12(1):18690.
                            doi: 10.1038/s41598-022-21350-wpubmed: 36333376google scholar: lookup
                          5. Paiva L, Silva M, Carrasco R, Ratto MH. The ovulatory and luteotropic actions of the male-derived beta-nerve growth factor in South American camelids.. Anim Front 2022 Aug;12(4):87-94.
                            doi: 10.1093/af/vfac037pubmed: 35974784google scholar: lookup
                          6. Ohaneje UL, Osuagwuh UI, Alvarez-Rodríguez M, Yánez-Ortiz I, Tabarez A, Palomo MJ. The Re-Addition of Seminal Plasma after Thawing Does Not Improve Buck Sperm Quality Parameters.. Animals (Basel) 2021 Dec 3;11(12).
                            doi: 10.3390/ani11123452pubmed: 34944229google scholar: lookup
                          7. Mateo-Otero Y, Fernández-López P, Delgado-Bermúdez A, Nolis P, Roca J, Miró J, Barranco I, Yeste M. Metabolomic fingerprinting of pig seminal plasma identifies in vivo fertility biomarkers.. J Anim Sci Biotechnol 2021 Nov 12;12(1):113.
                            doi: 10.1186/s40104-021-00636-5pubmed: 34772452google scholar: lookup
                          8. Kaya A, Dogan S, Vargovic P, Kutchy NA, Ross P, Topper E, Oko R, van der Hoorn F, Sutovsky P, Memili E. Sperm proteins ODF2 and PAWP as markers of fertility in breeding bulls.. Cell Tissue Res 2022 Jan;387(1):159-171.
                            doi: 10.1007/s00441-021-03529-1pubmed: 34762184google scholar: lookup
                          9. Bottino JP, Pérez-Clariget R, Rodriguez MGK, Ratto M, Ungerfeld R. Multiple matings modify the estrous length, the moment of ovulation, and the estradiol and LH patterns in ewes.. Anim Reprod 2021;18(3):e20210045.
                            doi: 10.1590/1984-3143-AR2021-0045pubmed: 34691265google scholar: lookup
                          10. Padilla L, López-Arjona M, Martinez-Subiela S, Rodriguez-Martinez H, Roca J, Barranco I. Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows.. J Anim Sci Biotechnol 2021 Sep 13;12(1):101.
                            doi: 10.1186/s40104-021-00620-zpubmed: 34511116google scholar: lookup
                          11. Fuentes-Albero MC, González-Brusi L, Cots P, Luongo C, Abril-Sánchez S, Ros-Santaella JL, Pintus E, Ruiz-Díaz S, Barros-García C, Sánchez-Calabuig MJ, García-Párraga D, Avilés M, Izquierdo Rico MJ, García-Vázquez FA. Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus).. Front Cell Dev Biol 2021;9:673961.
                            doi: 10.3389/fcell.2021.673961pubmed: 34336830google scholar: lookup
                          12. Rola LD, Buzanskas ME, Melo LM, Chaves MS, Freitas VJF, Duarte JMB. Assisted Reproductive Technology in Neotropical Deer: A Model Approach to Preserving Genetic Diversity.. Animals (Basel) 2021 Jun 30;11(7).
                            doi: 10.3390/ani11071961pubmed: 34209061google scholar: lookup
                          13. Rodriguez-Martinez H, Martinez EA, Calvete JJ, Peña Vega FJ, Roca J. Seminal Plasma: Relevant for Fertility?. Int J Mol Sci 2021 Apr 22;22(9).
                            doi: 10.3390/ijms22094368pubmed: 33922047google scholar: lookup
                          14. Crean AJ, Immler S. Evolutionary consequences of environmental effects on gamete performance.. Philos Trans R Soc Lond B Biol Sci 2021 Jun 7;376(1826):20200122.
                            doi: 10.1098/rstb.2020.0122pubmed: 33866815google scholar: lookup
                          15. Aisen EG, Huanca López W, Pérez Durand MG, Torres Mamani E, Villanueva Mori JC, Ousset MJ, Medina VH, Pérez Guerra UH, Huanca Mamani T. Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing.. Front Vet Sci 2021;8:611301.
                            doi: 10.3389/fvets.2021.611301pubmed: 33644145google scholar: lookup
                          16. Sari LM, Zampini R, Gonzalez Del Pino F, Argañaraz ME, Ratto MH, Apichela SA. Effects of NGF Addition on Llama (Lama glama) Sperm Traits After Cooling.. Front Vet Sci 2020;7:610597.
                            doi: 10.3389/fvets.2020.610597pubmed: 33479599google scholar: lookup
                          17. Tumova L, Zigo M, Sutovsky P, Sedmikova M, Postlerova P. Ligands and Receptors Involved in the Sperm-Zona Pellucida Interactions in Mammals.. Cells 2021 Jan 12;10(1).
                            doi: 10.3390/cells10010133pubmed: 33445482google scholar: lookup
                          18. El Kadili S, Kirschvink N, Raes M, Bister JL, Archa B, Douaik A, Chentouf M. Influence of Season and Liquid Storage at 16 °C on Beni Arouss Bucks' Semen Quality.. Animals (Basel) 2020 Oct 29;10(11).
                            doi: 10.3390/ani10111986pubmed: 33137921google scholar: lookup
                          19. Mahiddine FY, Kim JW, Qamar AY, Ra JC, Kim SH, Jung EJ, Kim MJ. Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters.. Animals (Basel) 2020 Oct 16;10(10).
                            doi: 10.3390/ani10101899pubmed: 33081332google scholar: lookup
                          20. Parrilla I, Martinez EA, Gil MA, Cuello C, Roca J, Rodriguez-Martinez H, Martinez CA. Boar seminal plasma: current insights on its potential role for assisted reproductive technologies in swine.. Anim Reprod 2020 Jul 21;17(3):e20200022.
                            doi: 10.1590/1984-3143-AR2020-0022pubmed: 33029213google scholar: lookup
                          21. Bubenickova F, Postlerova P, Simonik O, Sirohi J, Sichtar J. Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation.. Int J Mol Sci 2020 Sep 3;21(17).
                            doi: 10.3390/ijms21176415pubmed: 32899253google scholar: lookup
                          22. Pinet-Charvet C, Fleurot R, Derouin-Tochon F, de Graaf S, Druart X, Tsikis G, Taragnat C, Teixeira-Gomes AP, Labas V, Moreau T, Cayla X, Duittoz AH. Beta-nerve growth factor stimulates spontaneous electrical activity of in vitro embryonic mouse GnRH neurons through a P75 mediated-mechanism.. Sci Rep 2020 Jun 30;10(1):10654.
                            doi: 10.1038/s41598-020-67665-4pubmed: 32606357google scholar: lookup
                          23. Mateo-Otero Y, Fernández-López P, Gil-Caballero S, Fernandez-Fuertes B, Bonet S, Barranco I, Yeste M. (1)H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites.. Biomolecules 2020 Jun 15;10(6).
                            doi: 10.3390/biom10060906pubmed: 32549232google scholar: lookup
                          24. Mateo-Otero Y, Sánchez JM, Recuero S, Bagés-Arnal S, McDonald M, Kenny DA, Yeste M, Lonergan P, Fernandez-Fuertes B. Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression.. Front Cell Dev Biol 2020;8:341.
                            doi: 10.3389/fcell.2020.00341pubmed: 32478076google scholar: lookup
                          25. Dhar P, Samarasinghe RM, Shigdar S. Antibodies, Nanobodies, or Aptamers-Which Is Best for Deciphering the Proteomes of Non-Model Species?. Int J Mol Sci 2020 Apr 3;21(7).
                            doi: 10.3390/ijms21072485pubmed: 32260091google scholar: lookup
                          26. Green C, Rickard JP, de Graaf SP, Crean AJ. From One Ejaculate to Another: Transference of Sperm Traits via Seminal Plasma Supplementation in the Ram.. Biology (Basel) 2020 Feb 18;9(2).
                            doi: 10.3390/biology9020033pubmed: 32085377google scholar: lookup
                          27. Bergfelt DR, Blum JL, Ratner JR, Ratto MH, O'Brien JK, Robeck TR. PRELIMINARY EVALUATION OF SEMINAL PLASMA PROTEINS AND IMMUNOREACTIVITY OF NERVE GROWTH FACTOR AS INDICATIVE OF AN OVULATION INDUCING FACTOR IN ODONTOCETES.. J Zoo Biol 2018;2(1):21-29.
                            pubmed: 31799514
                          28. Fernandez-Fuertes B, Sánchez JM, Bagés-Arnal S, McDonald M, Yeste M, Lonergan P. Species-specific and collection method-dependent differences in endometrial susceptibility to seminal plasma-induced RNA degradation.. Sci Rep 2019 Oct 21;9(1):15072.
                            doi: 10.1038/s41598-019-51413-4pubmed: 31636362google scholar: lookup
                          29. Thélie A, Rehault-Godbert S, Poirier JC, Govoroun M, Fouchécourt S, Blesbois E. The seminal acrosin-inhibitor ClTI1/SPINK2 is a fertility-associated marker in the chicken.. Mol Reprod Dev 2019 Jul;86(7):762-775.
                            doi: 10.1002/mrd.23153pubmed: 31033055google scholar: lookup
                          30. Sepil I, Hopkins BR, Dean R, Thézénas ML, Charles PD, Konietzny R, Fischer R, Kessler BM, Wigby S. Quantitative Proteomics Identification of Seminal Fluid Proteins in Male Drosophila melanogaster.. Mol Cell Proteomics 2019 Mar 15;18(Suppl 1):S46-S58.
                            doi: 10.1074/mcp.RA118.000831pubmed: 30287546google scholar: lookup
                          31. Feugang JM, Liao SF, Willard ST, Ryan PL. In-depth proteomic analysis of boar spermatozoa through shotgun and gel-based methods.. BMC Genomics 2018 Jan 18;19(1):62.
                            doi: 10.1186/s12864-018-4442-2pubmed: 29347914google scholar: lookup
                          32. Wang X, Zhao X, Huang D, Pan X, Qi Y, Yang Y, Zhao H, Cheng G. Proteomic analysis and cross species comparison of casein fractions from the milk of dairy animals.. Sci Rep 2017 Feb 27;7:43020.
                            doi: 10.1038/srep43020pubmed: 28240229google scholar: lookup
                          33. Atikuzzaman M, Alvarez-Rodriguez M, Vicente-Carrillo A, Johnsson M, Wright D, Rodriguez-Martinez H. Conserved gene expression in sperm reservoirs between birds and mammals in response to mating.. BMC Genomics 2017 Jan 18;18(1):98.
                            doi: 10.1186/s12864-017-3488-xpubmed: 28100167google scholar: lookup
                          34. Defaus S, Avilés M, Andreu D, Gutiérrez-Gallego R. Identification of Bovine Sperm Surface Proteins Involved in Carbohydrate-mediated Fertilization Interactions.. Mol Cell Proteomics 2016 Jul;15(7):2236-51.
                            doi: 10.1074/mcp.M115.057703pubmed: 27094474google scholar: lookup
                          35. da Silva BF, Meng C, Helm D, Pachl F, Schiller J, Ibrahim E, Lynne CM, Brackett NL, Bertolla RP, Kuster B. Towards Understanding Male Infertility After Spinal Cord Injury Using Quantitative Proteomics.. Mol Cell Proteomics 2016 Apr;15(4):1424-34.
                            doi: 10.1074/mcp.M115.052175pubmed: 26814186google scholar: lookup
                          36. Plante G, Lusignan MF, Lafleur M, Manjunath P. Interaction of milk proteins and Binder of Sperm (BSP) proteins from boar, stallion and ram semen.. Reprod Biol Endocrinol 2015 Aug 15;13:92.
                            doi: 10.1186/s12958-015-0093-1pubmed: 26272219google scholar: lookup
                          37. Olejnik B, Jarząb A, Kratz EM, Zimmer M, Gamian A, Ferens-Sieczkowska M. Terminal Mannose Residues in Seminal Plasma Glycoproteins of Infertile Men Compared to Fertile Donors.. Int J Mol Sci 2015 Jul 2;16(7):14933-50.
                            doi: 10.3390/ijms160714933pubmed: 26147424google scholar: lookup
                          38. Mattei AL, Riccio ML, Avila FW, Wolfner MF. Integrated 3D view of postmating responses by the Drosophila melanogaster female reproductive tract, obtained by micro-computed tomography scanning.. Proc Natl Acad Sci U S A 2015 Jul 7;112(27):8475-80.
                            doi: 10.1073/pnas.1505797112pubmed: 26041806google scholar: lookup
                          39. McGraw LA, Suarez SS, Wolfner MF. On a matter of seminal importance.. Bioessays 2015 Feb;37(2):142-7.
                            doi: 10.1002/bies.201400117pubmed: 25379987google scholar: lookup
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