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Home / Equine Research Bank / Am J Vet Res / Quantitation of hyaluronic acid in equine synovia.
American journal of veterinary research1982; 43(6); 1096-1099;

Quantitation of hyaluronic acid in equine synovia.

Abstract: An assay for the determination of hyaluronic acid concentration in equine synovia (synovial fluid) was investigated. The method involved the degradation of hyaluronic acid, using a highly specific microbial enzyme. The unsaturated tetra- and hexasaccharide products were oxidized with periodate and caused to react with thiobarbituric acid for form a chromophore which absorbed in the visible range of the spectrum. The method was precise within acceptable limits for synovia and compared favorably with the widely used carbazole method of Bitter and Muir (Anal Biochem 4:330-334, 1962). The between-assay coefficient of variation was 1.7% and the method was found to be linear to 100 microgram of hyaluronic acid. The mean value for hyaluronic acid concentration in synovia of health horses was 127 mg/100 ml.
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Publication Date: 1982-06-01 PubMed ID: 7103180
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores a method for measuring the concentration of hyaluronic acid in the synovial fluid (the fluid in joints) of horses. The researched assay appears consistent with accuracy and precision.

Methodology

  • The research investigates an assay for measuring hyaluronic acid in equine synovial fluid (synovia).
  • The methodology involves the breakdown of hyaluronic acid utilizing a highly specific microbial enzyme.
  • The disintegrated hyaluronic acid results in unsaturated tetra- and hexasaccharide products.
  • These saccharide products then oxidize with a substance called periodate.
  • Post oxidation, these products react with a compound called thiobarbituric acid.
  • This reaction results in a chromophore, a compound that absorbs light within the visible spectrum.

Comparison and Precision

  • This method is tested against the widely used carbazole method by Bitter and Muir (1962) and proven to be precise within acceptable boundaries for synovial fluid.
  • There is very little variation in the assay, as indicated by a between-assay coefficient of variation of only 1.7%.
  • The test method can be linearly adapted to measure up to 100 micrograms of hyaluronic acid.

Application

  • The average concentration of hyaluronic acid in healthy horses’ synovia (100 ml) is found to be 127 mg.

Overall, this researched assay to quantify hyaluronic acid concentration in equine synovia demonstrated precise, reliable results, and compared favorably to pre-existing methods.

Cite This Article

APA
Rowley G, Antonas KN, Hilbert BJ. (1982). Quantitation of hyaluronic acid in equine synovia. Am J Vet Res, 43(6), 1096-1099.

Publication

American journal of veterinary research
ISSN: 0002-9645
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 43
Issue: 6
Pages: 1096-1099

Researcher Affiliations

Rowley, G
    Antonas, K N
      Hilbert, B J

        MeSH Terms

        • Animals
        • Arthritis / metabolism
        • Arthritis / veterinary
        • Biological Assay / methods
        • Horse Diseases / metabolism
        • Horses / metabolism
        • Hyaluronic Acid / analysis
        • Hyaluronic Acid / metabolism
        • Polysaccharide-Lyases / metabolism
        • Synovial Fluid / analysis
        • Synovial Fluid / metabolism

        Citations

        This article has been cited 5 times.
        1. Grissom MJ, Temple-Wong MM, Adams MS, Tom M, Schumacher BL, McIlwraith CW, Goodrich LR, Chu CR, Sah RL. Synovial Fluid Lubricant Properties are Transiently Deficient after Arthroscopic Articular Cartilage Defect Repair with Platelet-Enriched Fibrin Alone and with Mesenchymal Stem Cells. Orthop J Sports Med 2014 Jul;2(7).
          doi: 10.1177/2325967114542580pubmed: 25530978google scholar: lookup
        2. Antonacci JM, Schmidt TA, Serventi LA, Cai MZ, Shu YL, Schumacher BL, McIlwraith CW, Sah RL. Effects of equine joint injury on boundary lubrication of articular cartilage by synovial fluid: role of hyaluronan. Arthritis Rheum 2012 Sep;64(9):2917-26.
          doi: 10.1002/art.34520pubmed: 22605527google scholar: lookup
        3. Wong BL, Kim SH, Antonacci JM, McIlwraith CW, Sah RL. Cartilage shear dynamics during tibio-femoral articulation: effect of acute joint injury and tribosupplementation on synovial fluid lubrication. Osteoarthritis Cartilage 2010 Mar;18(3):464-71.
          doi: 10.1016/j.joca.2009.11.008pubmed: 20004636google scholar: lookup
        4. Palmer JL, Bertone AL, McClain H. Assessment of glycosaminoglycan concentration in equine synovial fluid as a marker of joint disease. Can J Vet Res 1995 Jul;59(3):205-12.
          pubmed: 8521354
        5. Hutadilok N, Ghosh P, Brooks PM. Binding of haptoglobin, inter-alpha-trypsin inhibitor, and alpha 1 proteinase inhibitor to synovial fluid hyaluronate and the influence of these proteins on its degradation by oxygen derived free radicals. Ann Rheum Dis 1988 May;47(5):377-85.
          doi: 10.1136/ard.47.5.377pubmed: 2455485google scholar: lookup
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