Radioimmunoassay of inhibin in various mammals.
Abstract: A sensitive radioimmunoassay (RIA) for the determination of inhibin in peripheral plasma and tissue homogenates of different species has been developed using antisera to partially purified bovine follicular fluid (bFF) inhibin and 125I-labelled bFF 32 kDa inhibin. Antisera were produced by immunization of rabbits with partially purified bFF inhibin prepared by immunoaffinity chromatography. Increasing doses of a high titre antiserum could neutralize the suppressing effect of bFF, porcine follicular fluid and rat ovarian homogenate on FSH secretion from rat anterior pituitary cells in culture. Sensitivity of the assay was 3.1 ng International Research Standard of porcine inhibin per tube. Parallel inhibition curves were obtained for inhibin preparations from female and male animals of ten species, i.e. cattle, goats, sheep, cats, dogs, monkeys, pigs, horses, rats and man. Inhibin subunits and related proteins cross-reacted minimally with the antiserum used in the study. Plasma concentrations of inhibin in adult male and female rats were measured by the RIA before and at various times after gonadectomy. Inhibin levels in peripheral plasma before gonadectomy were significantly higher in adult female than in adult male rats. Inhibin levels decreased abruptly after gonadectomy in both sexes and they correlated negatively with plasma concentrations of FSH. This inhibin RIA will facilitate studies of the physiology of inhibin in various species of animals.
Publication Date: 1989-09-01 PubMed ID: 2509617DOI: 10.1677/joe.0.1220697Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research presents a newly developed sensitive radioimmunoassay for the measurement of inhibin in the plasma and tissue homogenates across multiple species. The generated data show notable differences in inhibin concentrations between male and female rats, both pre and post-gonadectomy, thus suggesting potential for further studies on inhibin physiology.
Radioimmunoassay Development and Use
- The researchers developed a radioimmunoassay (RIA) for the purpose of quantifying inhibin in various mammals. Inhibin is a hormone that inhibits the production of follicle-stimulating hormone (FSH) in the pituitary gland.
- The RIA was created utilizing antisera formulated with partially purified bovine follicular fluid inhibin. This fluid was amassed through a technique called immunoaffinity chromatography, which helps separate various molecules via their reciprocal actions with an antibody. This fluid was then labelled with an iodine-125 radioisotope for detection in the RIA.
- The developed RIA displayed proven sensitivity, as shown by its capability to neutralize the suppressing effect of various follicular fluids and homogenates on FSH secretion from rat pituitary cells in culture.
Inhibin Levels in Various Mammals
- The RIA yielded parallel inhibition curves for inhibin preparations from both female and male animals across ten different species.
- Inhibin subunits and related proteins showed minimal cross-reactivity with the antiserum used in the study. This implies the specificity of the antiserum towards inhibin.
- The research documented different inhibin concentrations in adult male and female rats, both before and after gonadectomy. Female rats showed significantly higher inhibin levels before gonadectomy compared to the male rats.
- After gonadectomy, a surgical procedure of removing gonads, inhibin levels drastically dropped in both sexes. The levels of inhibin correlated negatively with the plasma concentrations of FSH, further demonstrating the inhibitory control of inhibin over FSH.
Importance of the Developed Radioimmunoassay
- This novel RIA provides an accurate, reliable method for measuring the concentrations of inhibin, which inhibits the production of FSH, thus giving more insight into reproductive processes.
- The development of this RIA lays the foundation for further research on inhibin’s physiology in a wide range of species.
Cite This Article
APA
Hamada T, Watanabe G, Kokuho T, Taya K, Sasamoto S, Hasegawa Y, Miyamoto K, Igarashi M.
(1989).
Radioimmunoassay of inhibin in various mammals.
J Endocrinol, 122(3), 697-704.
https://doi.org/10.1677/joe.0.1220697 Publication
Researcher Affiliations
- Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Japan.
MeSH Terms
- Animals
- Castration
- Cattle
- Cross Reactions
- Dose-Response Relationship, Immunologic
- Female
- Follicle Stimulating Hormone / blood
- Humans
- Inhibins / blood
- Luteinizing Hormone / blood
- Male
- Radioimmunoassay / methods
- Rats
Citations
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