Rapid analysis of fatty acid-binding proteins with immunosensors and immunotests for early monitoring of tissue injury.
Abstract: Fatty acid-binding protein (FABP) holds promise for early detection of tissue injury. This small protein (15kD) appears earlier in the blood than large proteins after cell damage. Combined its characteristics of high concentration tissue contents and low normal plasma values provide the possibility of a rapid rise above the respective reference values, and thus an early indication of the appearance of tissue injury. A general review was presented on the current status of different types of FABP for the detection of tissue injury in patients with myocardial injury, brain injury and also in athletes or horses with skeletal muscle injury. To take full advantage of the characteristics of the early marker FABP, rapid analysis is a crucial parameter. In this review, an overview of the development of immunoassay for the quantification of FABP in buffer, plasma or whole blood was outlined. The characteristics of different FABP immunosensors and immunotests were described. The feasibility of these immunoassays to be used in routine clinical practice and in emergency case was also discussed. Nowadays, the improved automated immunoassays (e.g. a microparticle-enhanced turbidimetric immunoassay), less time-consuming bedside immunosensors and immunotests (e.g. a one-step FABP lateral flow immunotest), are the main advance technology in point-of-care testing. With these point-of-care tests, the application of FABP as an early tissue injury marker has a great potential for many clinical purposes.
Publication Date: 2004-11-18 PubMed ID: 15854826DOI: 10.1016/j.bios.2004.09.028Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Review
Summary
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This research review focuses on the role and detection methods of fatty acid-binding proteins (FABPs) in indicating early tissue injury. The promising advances in immunosensors and immunotests, including less time-consuming and automated methods, are highlighted as key techniques in rapid and efficient detection of FABP levels for clinical use.
Significance of Fatty Acid-Binding Protein (FABP) in Detecting Tissue Injury
- The small size of FABP allows it to appear in the blood quicker than larger proteins following tissue injury, making it a potential early indicator of cell damage.
- Under normal conditions, FABP has low plasma values, but high tissue concentration. Therefore, a significant increase in plasma FABP indicates tissue damage.
- The review covers the use of various types of FABP detection in patients with myocardial (heart) injury, brain injury, and in athletes or horses with skeletal muscle injury.
Advances in FABP Detection Methods
- Rapid analysis of FABP is crucial to take full advantage of its use as an early marker of tissue injury.
- Several methods for the quantification of FABP in buffer, plasma or whole blood have been developed over the years.
- These methods have evolved to include immunosensors and immunotests that are specifically designed to detect FABP.
- The attributes of various FABP immunosensors and immunotests have been assessed for their feasibility in routine clinical practice and emergency cases.
The Future of FABP in Point-of-Care Testing
- Automated and less time-consuming immunoassays, such as a microparticle-enhanced turbidimetric immunoassay, present a substantial advancement in point-of-care testing.
- One-step FABP lateral flow immunotests are identified as effective bedside immunosensors that can increase efficiency in the clinical setting.
- With these advancements in point-of-care tests, the use of FABP as an early tissue injury marker has significant potential for various clinical applications.
Cite This Article
APA
Chan CP, Wan TS, Watkins KL, Pelsers MM, Van der Voort D, Tang FP, Lam KH, Mill J, Yuan Y, Lehmann M, Hempel A, Sanderson JE, Glatz JF, Renneberg R.
(2004).
Rapid analysis of fatty acid-binding proteins with immunosensors and immunotests for early monitoring of tissue injury.
Biosens Bioelectron, 20(12), 2566-2580.
https://doi.org/10.1016/j.bios.2004.09.028 Publication
Researcher Affiliations
- Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, PR China.
MeSH Terms
- Animals
- Biomarkers / blood
- Biosensing Techniques / instrumentation
- Biosensing Techniques / methods
- Blood Chemical Analysis / instrumentation
- Blood Chemical Analysis / methods
- Carrier Proteins / blood
- Connective Tissue Diseases / blood
- Connective Tissue Diseases / diagnosis
- Equipment Design
- Fatty Acid-Binding Proteins
- Humans
- Immunoassay / instrumentation
- Immunoassay / methods
- Monitoring, Physiologic / instrumentation
- Monitoring, Physiologic / methods
- Myocardial Infarction / blood
- Myocardial Infarction / diagnosis
Citations
This article has been cited 8 times.- Hendriks S, Stokmans SC, Plas M, Buurman WA, Spoorenberg SLW, Wynia K, Heineman E, van Leeuwen BL, de Haan JJ. Compromised intestinal integrity in older adults during daily activities: a pilot study. BMC Geriatr 2021 Nov 4;21(1):628.
- Voth M, Lustenberger T, Relja B, Marzi I. Is I-FABP not only a marker for the detection abdominal injury but also of hemorrhagic shock in severely injured trauma patients?. World J Emerg Surg 2019;14:49.
- Singh A, Pramanik A, Acharya P, Makharia GK. Non-Invasive Biomarkers for Celiac Disease. J Clin Med 2019 Jun 21;8(6).
- Voth M, Duchene M, Auner B, Lustenberger T, Relja B, Marzi I. I-FABP is a Novel Marker for the Detection of Intestinal Injury in Severely Injured Trauma Patients. World J Surg 2017 Dec;41(12):3120-3127.
- Gerede DM, Güleç S, Kiliçkap M, Kaya CT, Vurgun VK, Özcan ÖU, Göksülük H, Erol Ç. Comparison of a qualitative measurement of heart-type fatty acid-binding protein with other cardiac markers as an early diagnostic marker in the diagnosis of non-ST-segment elevation myocardial infarction. Cardiovasc J Afr 2015 Nov-Dec;26(6):204-9.
- Conroy DJ, Millner PA, Stewart DI, Pollmann K. Biosensing for the environment and defence: aqueous uranyl detection using bacterial surface layer proteins. Sensors (Basel) 2010;10(5):4739-55.
- Makhsous M, Lin F, Pandya A, Pandya MS, Chadwick CC. Elevation in the serum and urine concentration of injury-related molecules after the formation of deep tissue injury in a rat spinal cord injury pressure ulcer model. PM R 2010 Nov;2(11):1063-5.
- Liyan C, Jie Z, Xiaozhou H. Prognostic value of combination of heart-type fatty acid-binding protein and ischemia-modified albumin in patients with acute coronary syndromes and normal troponin T values. J Clin Lab Anal 2009;23(1):14-8.
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